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3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

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Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology<br />

P12 ThE EFFECT OF FERMENTATION<br />

CONDITIONS ON ThE yEAST AROMA<br />

PROFILE<br />

JAROSLAVA KAňUCHOVá PáTKOVá a , EMíLIA<br />

BREIEROVá b , KORnéLIA nEMCOVá a and InGRID<br />

VAJCZIKOVá c<br />

a Institute for viticulture and enology of SARC<br />

Matuškova 25, 831 01 Bratislava, Slovak republic,<br />

b Institute of Chemistry SAS, Dubravska cesta 9, 845 38 Bratislava,<br />

Slovak republic,<br />

chememi@savba.sk<br />

Introduction<br />

Several factors influence the aroma profile during the<br />

alcoholic fermentation of the grape must. One of the widely<br />

discussed topics is the using of apiculate microflora and its<br />

s599<br />

contribution to the aroma of wine. Several authors have studied<br />

1–3 the behaviour of various yeast genera – Kloeckera,<br />

Pichia, Bretanomyces and their contribution to the wine<br />

aroma, considering the apiculate microflora as spoilage. Our<br />

previous results 4 confirmed the role of apiculate microflora<br />

in the wine aroma production under semiaerobic conditions<br />

and support of the original character of wine by apiculate<br />

microflora.<br />

The aim of this work was to determine the effect of<br />

changes in external conditions – oxygen, temperature or substrate<br />

composition on the sensorial expression of genera Rhodotorula,<br />

Sporobolomyces, Pichia, Hansenula Issat-chenkia,<br />

and Torulospora under the winemaking process. All above<br />

mentioned factors influence positively or negatively the final<br />

wine product. Multivariate analysis of variance was used to<br />

determine the overall weight of each one factor.<br />

Table I<br />

Sensorial evaluation of fermented grape must by various yeast strains under various fermentation conditions<br />

Anaerobic conditions Anaerobic conditions<br />

grape must synthetic medium<br />

Yeast strain<br />

10°C<br />

aroma percept aroma<br />

25°C<br />

percept<br />

10°C<br />

aroma percept<br />

25°C<br />

aroma percept<br />

C. intermediata yeast + sulphuric – grape + acetic –<br />

R.mucilaginosa socks smelly – yeast + yeast + socks smelly –<br />

T.delbruecki yeast + acetic – sweety + bread –<br />

S. cerevisiae acidic – fruity + sulphuric – sulphuric –<br />

I. orientalis autolyses – acethone – yeast – not recognised –<br />

Pichia anomala acethone – acidic – fruity + acethone –<br />

S.cerevisiae yeast – sulphuric – fruity + acethone –<br />

P. membranofaciens autolyses – sulphuric – socks smelly – not recognised –<br />

R.mucilaginosa autolyses – acidic – corny + sweet –<br />

P. anomala acethone – fruity– apple + candy floss + not recognised –<br />

Sp.pararoseus not recognised + fruity + socks smelly – acethone –<br />

S.cerevisiae acidic – sulphuric – rubber smell – autolyses –<br />

S.cerevisiae fruity + sulphuric – acidic + autolyses –<br />

Semiaerobic conditions Semiaerobic conditions<br />

grape must synthetic medium<br />

Yeast strain<br />

10°C<br />

aroma percept aroma<br />

25°C<br />

percept<br />

10°C<br />

aroma percept<br />

25°C<br />

aroma percept<br />

C. intermediata acidic – fruity + floral + fruity +<br />

R. mucilaginosa acethone – fruity + frowstilly – vanilla +<br />

T.delbruecki vanilla + vanilla + honey + fruity +<br />

S. cerevisiae autolyses – grape + sulphuric – acetic –<br />

I. orientalis acethone – fruity + autolyses – autolyses –<br />

P. anomala honey + fruity + fruity + fruity +<br />

S. cerevisiae honey + acetic – yeast + floral +<br />

P. membranofaciens yeast + acetic – medical – yeast +<br />

R.. mucilaginosa honey + fruity + sulphuric – not recognise –<br />

P. anomala honey + fruity – sweety + yeast +<br />

S. pararoseus yeast + fruity + sweety + not recognise +<br />

S. cerevisiae yeast + yeast + sweety + not recognised +<br />

S. cerevisiae yeast + fruity – not recognised + not recognised +<br />

S. cerevisiae yeast + fruity – autolyses – not recognised +

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