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3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

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Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology<br />

The influence of extraction temperature on CIE L * , a * , b *<br />

colour characteristics or on pH values was only negligible.<br />

S t a t i s t i c a l E v a l u a t i o n<br />

All the above mentioned characteristics were used for<br />

the classification and mutual recognition of both wine grape<br />

varieties using the principal component analysis (Fig. <strong>3.</strong>) and<br />

canonical discrimination analysis 22 .<br />

Both statistical approaches provided practically 100%<br />

correct classification of both varieties. As also follows from<br />

discriminant analysis, all the monitored characteristics are<br />

dependent on the mass of grape skins used for respective extract<br />

preparation.<br />

Additional experiments are in progress to evaluate the<br />

influence of solvent on the above-mentioned characteristics<br />

as well as to analyze the profile of polyphenols in both grape<br />

skin varieties.<br />

Conclusions<br />

Multi-experimental analysis performed with methanolic<br />

extracts prepared from grape skins of two varieties (St. Laurent<br />

and Alibernet) by PFE, revealed their significant antioxidant<br />

and radical-scavenging abilities, in significantly positive<br />

correlation with the TPC content. The influence of extraction<br />

temperature on radical – scavenging abilities, tristimulus values<br />

or TPC content is non-significant.<br />

Czech Science Foundation (Project No. GA203/08/1536),<br />

Academy of Sciences of the Czech Republic (Institutional<br />

Research Plan No. AV0Z40310501) and Science and Research<br />

Assistant Agency (Project APVV SK – CZ 0072 – 07) are<br />

gratefully acknowledged for the financial support. Authors<br />

would like to express their thanks to Dr. Milan Suhaj (Food<br />

Research Institute, Bratislava) for statistical evaluation of<br />

experimental data and visualisations.<br />

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