3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
Finally appropriated method for releasing the bacterial
cells from disc had to be worked out. The ultrasonic bath was
used and the working time was varied up to 8 minutes. The
amount of released bacteria and effect of ultrasonic waves
on the tested material was evaluated see Fig. 4. The best results
were obtained for the working time of 6 minutes. Longer
working time causes irreversible material damage while
shorter treatment time did not released all bacteria.
Fig. 4. Ceramic materials: A = without s. mutans, B = with
s. mutans, C = after ultrasonig bath for 6 minutes
The main goal of this work was to find out appropriate
method for the studying of the microbial adhesion to new
dental materials. The spectrofotometric techniques based on
Biuret reagent were employed. The method was optimised in
order to fulfill requirements for further material testing.
According to our measurement the BHIB medium with
1 wt% of glucose gives the best growing results. Samples
placed in BHIB-glucose medium was further inoculated
with 0,2 ml of S. mutans suspension. This volume fulfil
both requirement – low volume and good growing results.
Further the appropriate incubation time was investigated. The
highest absorbance was obtained for the samples incubated
for 18 hours.
s770
Finally time neccessary for releasing bacteria from the
disc samples in ultrasonic bath was evaluated. The best results
were obtained after 6 minutes. The highest amount of bactery
was released while the disc still remained undamaged.
This optimised method will be employed for testing new
dental materials. Our further studies will be focused on testing
various antimicrobial additives that will improve microbial
resistance of dental restorative materials.
This work has been supported by Ministry of Education,
Youth and Sports under project MSM 021630501.
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