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3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

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Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology<br />

Results<br />

Fig. 2. shows biomass and pullulan production in presence<br />

of modified PUR foams. It seems that the highest pullulan<br />

production was observed in control medium (without<br />

PUR), so, PUR presence exhibited negative effect on pullan<br />

production.<br />

g dm -3<br />

14<br />

12<br />

10<br />

8<br />

6<br />

4<br />

2<br />

0<br />

control REF PUR 10% ACS 10% HEC 10% AC 10% CMC<br />

biomass (g dm-3)<br />

pullulan (g dm-3)<br />

Fig. 2. biomass and pullulan production by A. pullulans grown<br />

in presence of modified PuR foams<br />

Conclusions<br />

Yeast strain Aureobasidium pullulans can grow in presence<br />

of modified polyurethane foams. Degradation degree<br />

of BIO-PUR corresponded with growth of yeast culture. The<br />

highest degree of degradation was found in PUR modified by<br />

s748<br />

40 % carboxymethyl cellulose; degree of degradation decreased<br />

with CMC concentration.<br />

According to our results of A. pullulans grown in PUR<br />

presence it seems that most of modified polyurethane foams<br />

exhibited slight negative effect on pullullan (4.3 g dm –3 control,<br />

4.9 g dm –3 1% ACS-PUR, <strong>3.</strong>2 g dm –3 REF-PUR) as well<br />

as biomass production (11.5 g dm –3 control, 7.8 g dm –3 1%<br />

ACS-PUR, 8.9 g dm –3 REF-PUR). Acetyl cellulose was the<br />

only modification agent, which was able to enhance pullulan<br />

(6.5 g dm –3 ) as well as biomass (12.5 g dm –3 ) production.<br />

This work was supported by project MSM 0021630501<br />

of the Czech Ministry of Education, Youth and Sports.<br />

REFEREnCES<br />

1. Leathers T. D.: Appl Microbiol Biotechnol. 62, 468<br />

(2003).<br />

2. Lee J. H., Kim J. H., Zhu I. H., Zhan X. B., Lee J. W.,<br />

Shin D. H. et al.: Biotechnol Lett. 23, 817 (2001).<br />

<strong>3.</strong> Shingel K. I.: Carbohydrate Res. 339, 447 (2004).<br />

4. Audet J., Lounes M., Thibault J.: Bioprocess. Eng. 15,<br />

209 (1996).<br />

5. Káš J., Kodíček M., Valentová O.: Laboratorni techniky<br />

biochemie. Praha 2005.

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