3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
Table I
Growth rate of A. pullulans on different cultivations media
– glucose medium (GM), glucose medium with wheat straw
(GSM) and basal medium with wheat straw (BSM)
Incubation
time
[days]
Type of
inoculation
Solid state fermentation
GM GSM BSM
26/32 ˚C 26/32 ˚C 26/32 ˚C
Loop 0/+ 0/0 0/+
3 Fungal spores +/+ +/+ 0/+
Mycelial discs 0/0 0/0 0/0
Loop 0/+ 0/0 0/+
5 Fungal spores +/+ +/+ +/+
Mycelial discs +/0 +/0 +/0
Loop 0/+ ++/0 ++/+
10 Fungal spores ++/+ ++/0 ++/+
Mycelial discs +/+ +/0 ++/+
Loop 0/+ ++/+ +/+
14 Fungal spores ++/+ ++/+ ++/+
Mycelial discs +/+ +/+ ++/++
0 = no growth, + = growth, ++ = extremelly growth
fermentation, A. pullulans was studied in various solid and
liguid media. no laccase activity could be detected in any
solid media. In contrast to solid-state fermentation, this strain
proceeded the maximum activity in liquid GM, but low level
of laccase was detected in BSM. Laccase production reached
its maxima on 8 and 15 day for GM and 7 day for GSM and
BSM. (Fig. 1.).
Fig. 1. Comparison of the production of laccase by A. pullulans
grown on three different culture media during submerged
cultivation
s746
Conclusions
Our results indicated, that the conditions optimal for
A. pullulans growth were determined for glucose medium
with wheat straw, temperature 26 °C, inoculation by fungal
spores and solid state fermentation. The results reported here
also showed that optimal growth of A. pullulans and production
of laccase were not be detected for the same cultivation
conditions.
During the optimalization of the culture medium hydroxylase
activity reached higher level in the presence of yeast
Aureobasidium pulllulans in the glucose medium without
wheat straw during submerged cultivation. Relatively higher
laccase production on GM showed twin peaks during their
incubation.
Reapperance of enzyme activity during later stage of
wheat straw degradation might be attributed to fungal autolysis
resulting in the release of cell membrane bound on intracellular
enzymes in the medium 10 .
This work has been supported by Ministry of Education,
Youth and Sports under project MSM 021630501.
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