3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
mycotoxins, moreover, isolation of same metabolite of parcial
toxins is possible depending on cross reactivity of particular
antibodies. Thanks to removing most of interferences
with ionization process (resulting in signal suppression) and
on this account, a lower detection limits can be obtained in
LC-MS/MS. In addition, the improvement of method performance
characteristics, the usage of standards in net solvent is
possible for calibration. On the other hand, the cost of analysis
is fairly increased by using immunoaffinity columns. Therefore,
for analysis of sample containing relatively high levels
of mycotoxins such as maize used in this study, the direct
analysis of crude extract is better option. Moreover, more
accurate quantification of D3G is obtained in this extract. As
far as, usage of IACs is prefered to obtained high sensitivity
s702
of analysis then DZT Multi MyCo IACs are recommended
for reliable pre-concentration of trichothecenes and ZOn.
This study was carried out with support from
MYKOTOXINY project (2B08049) – National program of
research II.
REFEREnCES
1. Berthiller F., Dall’Asta Ch., Schuhmacher R., Lemmens
M., Adam G., Krska R.: J. Agric. Food Chem. 53, 3421
(2005).
2. Lattanzio V. M. T., Solfrizzo M., Powers S., Visconti A.:
Rapid Commun. Mass Spectrom. 21, 3253 (2007).