3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
was extracted with hexane: ethyl acetate (9 : 1). The organic
layer was washed with 5% sodium chloride solution and then
evaporated to dry and dissolved in methanol. HPLC system
contained LC20 AT Shimadzu pumps and a Waters 990
PDA detector. A Supelco Discovery C18 (25 cm × 4.6 mm,
5 μm particle diameters) HPLC column was used. The
mobile phase consisted in methanol: water (98 : 2) and the
chromatogram was monitored at 292 nm. Tocopherol identification
was based on the retention time of α-tocopherol
standard (Sigma) and UV-VIS spectra. Quantitative analysis
was done using a calibration curve with α-tocopherol
(0.01–0.2 mg ml –1 ), r = 0.989.
Results
The results obtained for the sea buckthorn oil by GC-MS
are presented in Table I. The results obtained for sea buckthorn
vitamin E at different doses are shown in Table II.
Table II
Vitamin E determination in sea buckthorn oil by HPLC at
different doses [kGy]
Dose [kGy] α-tocopherol [mg 100 ml oil –1 ]
0 57.75
0.03 50.52
0.07 29.1
0.38 26.8
0.72 25.9
1.05 25.8
Fig. 2. The ratio between unsaturated and saturated fatty acids
evolution with doses [kGy] in sea buckthorn oil
Vitamin E decreased two times at 1.05 kGy treatment
(Table II). In sea buckthorn oil, at the highest dose applied,
the ratio between unsaturated and saturated fatty acids has
decreased at half value of control (Fig. 2.). Also the sterols
and vitamin E values have decreased with irradiation more
than two times, as presented in Fig. 4. Similar results have
been obtained in juice of the fruits. In sea buckthorn leaves
the changes are not significant (Fig. 3. and Fig. 5.).
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Fig. 3. The ratio between unsaturated and saturated fatty acids
evolution with doses [kGy] in sea buckthorn leaves
Fig. 4. Vitamin E and sitosterol [% weight] versus applied
doses (GC-MS determination)
Fig. 5. E vitamin and sterols [area %] with doses [kGy] in sea
buckthorn leaves
Conclusions
The GC-MS and HPLC methods developed showed significant
changes of antioxidants as vitamins E, F and sterols
with doses after the treatment of sea buckthorn oil, juice but
not in leaves at different kGy doses with accelerated electrons.
The treatment for decontamination needs to be optimized to
avoid loses of its important nutrients.
This work has been supported by the Romanian Research
Foundation (CEEX, project number 761/2006).
REFEREnCES
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3. Eccleston C., Yang B., Tahvonen R., Kalli, H., Rimbach
G. H., Minihan A. M.: J. nutr. Biochem. 13, 346 (2002).
4. Yang B., Kallio H.: Trends Food Sci. Technol. 13, 160
(2002).