3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
(on an average 4 %) were colonized as long as the rates are higher
than 8 ml min –1 (probably the influence of shear stress).
8–16 % area was colonized using the flow rates between 6
and 8 ml min –1 . The increase of shear stress had not significant
influence on morphological parameters of objects
– width and elongation with exception of length and area of
objects.
Conclusions
The capacity of Trichosporon cutaneum cells to colonize
cellophane (used as a carrier and the source of carbon)
was proved (Fig. 4.). FC 81 Flow Cell appears to be suitable
apparatus for microscopy study of biofilm formation. Image
analysis is favourable tool for monitoring and evaluation of
biofilm formation.
The flow rate 10 ml min –1 was appropriate for study of
biofilm formation. However, for the purpose of cellophane
degradation more colonized areas are required (6 ml min –1 ).
s656
The effect of shear stress is one of required information
needed for optimalization of these processes. nutrient starvation,
cell suspension density, medium composition etc. are
factors that could probably affect biofilm formation.
This work has been supported by project Eureka E !
3654 Biopols.
REFEREnCES
1. Lynd R. L., Weimer J. P., Zyl W. H., Pretorius I. S.:
Microbiol. Mol. Biol. Rev. 66, 505 (2002).
2. Šulák M., Šmogrovičová D.: Chem. listy 102, 108
(2008).
3. Rosenberg M. D., Gutnick E.: Fems Microbiol. Lett. 9,
29 (1980).
4. Yang X., Beienal H.,: J Microbiol Methods 39, 109
(2000).