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9<br />

Positive Effects of <strong>Wood</strong>-Inhabiting<br />

Microorganisms<br />

Particularly after the OPEC oil embargo of the 1970s, research turned towards<br />

the utilization of renewable resources like wood, yearly plants, and lignocellulosic<br />

waste from forestry and agriculture instead of oil as raw material for<br />

chemical and biological processes (“biotechnology of lignocelluloses”) (Eriksson<br />

et al. 1990; Dart and Betts 1991).<br />

Among the substantial causes that make the biological conversion of lignocelluloses<br />

difficult (Table 4.2), the most serious obstacle is the incrustation<br />

of the degradable carbohydrates cellulose and hemicelluloses by the lignin<br />

barrier, which is not surmountable by most microorganisms. Table 9.1 groups<br />

some bioconversions that have been done in the past or are recently investigated<br />

or already performed into those microbial processes, which go well<br />

directly with lignocelluloses, and into those, which need a pretreatment of the<br />

substrate. Only the wood-degrading white, brown, and soft-rot fungi, and the<br />

wood-degrading bacteria can degrade the native woody cell wall without any<br />

pretreatment of the substrate. Whereas brown and soft-rot fungi and assum-<br />

Table 9.1. Biotechnological procedures with lignocelluloses without and after substrate<br />

pretreatment<br />

conversion without substrate pretreatment<br />

– “myco-wood”<br />

– production of edible mushrooms<br />

– biological pulping<br />

pretreatment of the substrate and subsequent microbial conversion<br />

biological pretreatment<br />

– “palo podrido” and “myco-fodder”<br />

chemical pretreatment<br />

– hydrolysis of wood with acids and use of glucose for yeast production, ethanol<br />

fermentation and microbial transformations to amino acids, antibiotics, enzymes,<br />

vitamins<br />

– sulphite pulping process and use of hardwood pentoses in the spent liquor for yeast<br />

production and of softwood hexoses for ethanol fermentation<br />

– pulping and subsequent use of enzymes for deinking of waste paper<br />

physical pretreatment<br />

– grinding of lignocelluloses to improve accessibility to enzymes<br />

– steam explosion methods to open the wood structure for bioconversions<br />

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