21.12.2012 Views

Production Practices and Quality Assessment of Food Crops. Vol. 1

Production Practices and Quality Assessment of Food Crops. Vol. 1

Production Practices and Quality Assessment of Food Crops. Vol. 1

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

8 R. M. Madakadze <strong>and</strong> J. Kwaramba<br />

3.1.1. Causes <strong>of</strong> chilling injury<br />

The primary cause <strong>of</strong> chilling injury is thought to be damage to plant cell membranes.<br />

Changes in molecular species composition <strong>of</strong> phospholipids during cold<br />

storage <strong>and</strong> after rewarming were determined in mature green tomatoes (L’-Heureux<br />

et al., 1993) the minor molecular species shifted significantly towards unsaturation<br />

during cold storage. The presence <strong>of</strong> these polyunsaturated species <strong>and</strong> their<br />

breakdown products may induce membrane damage <strong>and</strong> dysfunction. The membrane<br />

damage sets <strong>of</strong>f a cascade <strong>of</strong> secondary reactions, which may include ethylene<br />

production, increased respiration, reduced photosynthesis, interference with energy<br />

production, accumulation <strong>of</strong> toxic compounds such as ethanol <strong>and</strong> acetaldehyde,<br />

electrolyte leakage <strong>and</strong> altered cellular structure (Skog, 1998).<br />

Some physiological parameters <strong>of</strong> chilling injury include electrolyte leakage,<br />

carbon dioxide <strong>and</strong> ethylene production in squash <strong>and</strong> cucumber (McCollum <strong>and</strong><br />

McDonald, 1993). These parameters increase with increasing severity <strong>of</strong> chilling<br />

injury (Lee <strong>and</strong> Yang, 1999). Electrolyte leakage was shown to increase during<br />

cold storage (1 °C for 2 days) <strong>of</strong> mature green tomato fruits (L’-Heureux et al., 1993).<br />

In bell peppers chilling injury was also accompanied by high internal carbon dioxide<br />

<strong>and</strong> ethylene production (Lin et al., 1993). Ethylene – forming enzyme activity<br />

can also be used as an indice for thermotolerance <strong>of</strong> crops (McCollum <strong>and</strong><br />

McDonald, 1993).<br />

Chilling injury can be measured non-destructively in fruits using pulse amplitude<br />

modulated fluorometry before tissue damage is visible (Lurie et al., 1994).<br />

Three photosynthetic characteristics could be measured by this method; quantum<br />

yield (F m/F o), photochemical quenching (Q p) <strong>and</strong> non-photochemical quenching (Q np).<br />

F m/F o decreased by 90% during the first week <strong>of</strong> storage at 2 °C <strong>and</strong> remained<br />

low thereafter, while Q np decreased after 2 weeks at 2 °C just before the fruits<br />

began to develop chilling injury. Q p was similar at both chilling <strong>and</strong> non-chilling<br />

storage temperature. Whether this method can be used in a field situation is not<br />

known.<br />

3.1.2. Symptoms <strong>of</strong> chilling injury<br />

Symptoms <strong>of</strong> chilling injury as summarized by Morris (1982) <strong>and</strong> Skog (1998)<br />

include the following:<br />

01. Surface lesions – pitting, large sunken areas, <strong>and</strong> discolouration. These<br />

symptoms occur most frequently in products with firm thick peel such as citrus<br />

or cucumber.<br />

02. Water soaking <strong>of</strong> tissues – this disruption <strong>of</strong> cell structure <strong>and</strong> accompanying<br />

release <strong>of</strong> substrates favours the growth <strong>of</strong> microorganism. Water soaking<br />

commonly occurs in vegetables with thin or s<strong>of</strong>t peels such as peppers <strong>and</strong><br />

asparagus.<br />

03. Water loss/desiccation/shrivelling<br />

04. Internal discolouration (browning) <strong>of</strong> pulp, vascular str<strong>and</strong>s, <strong>and</strong> seeds.<br />

05. Breakdown <strong>of</strong> tissues.

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!