Production Practices and Quality Assessment of Food Crops. Vol. 1
Production Practices and Quality Assessment of Food Crops. Vol. 1
Production Practices and Quality Assessment of Food Crops. Vol. 1
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8 R. M. Madakadze <strong>and</strong> J. Kwaramba<br />
3.1.1. Causes <strong>of</strong> chilling injury<br />
The primary cause <strong>of</strong> chilling injury is thought to be damage to plant cell membranes.<br />
Changes in molecular species composition <strong>of</strong> phospholipids during cold<br />
storage <strong>and</strong> after rewarming were determined in mature green tomatoes (L’-Heureux<br />
et al., 1993) the minor molecular species shifted significantly towards unsaturation<br />
during cold storage. The presence <strong>of</strong> these polyunsaturated species <strong>and</strong> their<br />
breakdown products may induce membrane damage <strong>and</strong> dysfunction. The membrane<br />
damage sets <strong>of</strong>f a cascade <strong>of</strong> secondary reactions, which may include ethylene<br />
production, increased respiration, reduced photosynthesis, interference with energy<br />
production, accumulation <strong>of</strong> toxic compounds such as ethanol <strong>and</strong> acetaldehyde,<br />
electrolyte leakage <strong>and</strong> altered cellular structure (Skog, 1998).<br />
Some physiological parameters <strong>of</strong> chilling injury include electrolyte leakage,<br />
carbon dioxide <strong>and</strong> ethylene production in squash <strong>and</strong> cucumber (McCollum <strong>and</strong><br />
McDonald, 1993). These parameters increase with increasing severity <strong>of</strong> chilling<br />
injury (Lee <strong>and</strong> Yang, 1999). Electrolyte leakage was shown to increase during<br />
cold storage (1 °C for 2 days) <strong>of</strong> mature green tomato fruits (L’-Heureux et al., 1993).<br />
In bell peppers chilling injury was also accompanied by high internal carbon dioxide<br />
<strong>and</strong> ethylene production (Lin et al., 1993). Ethylene – forming enzyme activity<br />
can also be used as an indice for thermotolerance <strong>of</strong> crops (McCollum <strong>and</strong><br />
McDonald, 1993).<br />
Chilling injury can be measured non-destructively in fruits using pulse amplitude<br />
modulated fluorometry before tissue damage is visible (Lurie et al., 1994).<br />
Three photosynthetic characteristics could be measured by this method; quantum<br />
yield (F m/F o), photochemical quenching (Q p) <strong>and</strong> non-photochemical quenching (Q np).<br />
F m/F o decreased by 90% during the first week <strong>of</strong> storage at 2 °C <strong>and</strong> remained<br />
low thereafter, while Q np decreased after 2 weeks at 2 °C just before the fruits<br />
began to develop chilling injury. Q p was similar at both chilling <strong>and</strong> non-chilling<br />
storage temperature. Whether this method can be used in a field situation is not<br />
known.<br />
3.1.2. Symptoms <strong>of</strong> chilling injury<br />
Symptoms <strong>of</strong> chilling injury as summarized by Morris (1982) <strong>and</strong> Skog (1998)<br />
include the following:<br />
01. Surface lesions – pitting, large sunken areas, <strong>and</strong> discolouration. These<br />
symptoms occur most frequently in products with firm thick peel such as citrus<br />
or cucumber.<br />
02. Water soaking <strong>of</strong> tissues – this disruption <strong>of</strong> cell structure <strong>and</strong> accompanying<br />
release <strong>of</strong> substrates favours the growth <strong>of</strong> microorganism. Water soaking<br />
commonly occurs in vegetables with thin or s<strong>of</strong>t peels such as peppers <strong>and</strong><br />
asparagus.<br />
03. Water loss/desiccation/shrivelling<br />
04. Internal discolouration (browning) <strong>of</strong> pulp, vascular str<strong>and</strong>s, <strong>and</strong> seeds.<br />
05. Breakdown <strong>of</strong> tissues.