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Production Practices and Quality Assessment of Food Crops. Vol. 1

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178 Chris A. Shisanya<br />

equation 3). The Rhizobia capable <strong>of</strong> nodulating TB in the soils <strong>of</strong> SE-Kenya was<br />

1.0 × 10 2 cells per gram <strong>of</strong> soil.<br />

2.9.2. Nodulation status <strong>and</strong> dry weight <strong>of</strong> TB in greenhouse experiment<br />

There were significant differences (P ≤ 0.05) in nodule number <strong>and</strong> hence nodule<br />

dry weight between the treatments (Table 4). Treatment R3254 had the highest<br />

number <strong>of</strong> nodules <strong>and</strong> hence nodule dry weight. At harvest, no significant differences<br />

in root <strong>and</strong> shoot dry weights between N <strong>and</strong> R3254 treatments were observed.<br />

However, these treatments differed significantly in the two parameters from the other<br />

treatments (Table 4).<br />

Table 4. Effectiveness <strong>of</strong> rhizobia strains in nitrogen fixation in tepary bean during greenhouse<br />

experiment.<br />

Treatment Nodule number Nodule dry Root dry Shoot dry<br />

(plant –1 ) weight weight weight<br />

(mg plant –1 ) (g plant –1 ) (g plant –1 )<br />

Nitrogen (N*) 03 c 02.8 d 0.7 a 5.6 a<br />

Control 00 d 00.0 e 0.2 c 1.5 c<br />

TB + R3254 40 a 17.8 a 0.8 a 5.8 a<br />

TB + R446 10 b 07.1 b 0.5 b 4.2 b<br />

TB + RTB 14 b 10.5 b 0.6 b 4.3 b<br />

TB + R578 04 c 03.0 d 0.5 b 3.8 b<br />

TB + R579 07 c 06.1 c 0.6 b 4.0 b<br />

Means (n = 7) followed by the same letter down the column are not significantly different (P ≤ 0.05)<br />

by Duncan’s multiple range test.<br />

* Nitrogen medium incorporating 5 mM Ca(NO 3) 2 4H 2O in the solution.<br />

(Source: after Shisanya, 2002)<br />

2.9.3. The LR season field results<br />

The total amount <strong>of</strong> rainfall received during the growing season was 223 mm.<br />

There were significant differences (P ≤ 0.05) in nodule number <strong>and</strong> the corresponding<br />

nodule dry weight between treatment R3254 <strong>and</strong> the rest <strong>of</strong> the other<br />

treatments (Table 5). Treatment R3254 had the highest number <strong>of</strong> nodules at this<br />

stage <strong>of</strong> growth. The rest <strong>of</strong> the treatments had the same number <strong>of</strong> nodules.<br />

However, no significant differences in total plant dry weight between the different<br />

treatments, 21 DAE, were observed (Table 5).<br />

The pattern observed 21 DAE was similar to that at 42 DAE, except for the<br />

pod dry weight <strong>and</strong> plant dry weight (Table 6). Treatment R3254 had significantly<br />

(P ≤ 0.05) higher pod numbers per plant <strong>and</strong> hence pods dry weight. There was<br />

no significant difference (P ≤ 0.05) in plant dry weight between the N <strong>and</strong> R3254<br />

treatments. The other treatments had similar plant dry weights (Table 6). At final<br />

harvest, the R3254 treatment had significantly (P ≤ 0.05) higher pod dry weight,<br />

plant dry weight, 100 seed weight, seed weight per plot <strong>and</strong> grain yield (Table 7).

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