Hilic Columns for the Analysis of Steviol Glycosides - eustas en
Hilic Columns for the Analysis of Steviol Glycosides - eustas en Hilic Columns for the Analysis of Steviol Glycosides - eustas en
What’s Next? ACN Usage = 3.5 mL/run Synergi Hydro-RP HST 100 x 2.00 mm, 2.5 µm particle size mAU 80 70 60 50 40 30 20 10 0 mAU 80 70 60 50 40 30 20 10 0 mAU 70 60 50 40 30 20 10 0 DAD1 D, Sig=210,8 Ref =600,100 (X:\XAVIER\DATA\2009\H0109\H0109_23.D) 1 2 3 4 5 6 7 8 Figure 8 Mill-Q water UV, 210 nm 8 9 10 11 12 Phenomenex C18(2)-HST 100 x 2.00 mm, 2.5 µm particle size DAD1 D, Sig=210,8 Ref =600,100 (X:\XAVIER\DATA\2009\H0109\H0109_13.D) 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 7 8 9 10 11 DAD1 D, Sig=210,8 Ref =600,100 (X:\XAVIER\DATA\2009\H0109\H0109_27.D) 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 10 10 10 10 10 11 12 Figure 9 Milli-Q water UV, 210 nm Phenomenex Fusion-HST 100 x 2.00 mm, 2.5 µm particle size 8 9 10 11 12 11 11 12 12 Figure 10 Milli-Q water UV, 210 nm 11 11 12 12 min min min Hoekstra et al., 2009 (Chromadex) 8
RP by Geuns Grace Alltima C18 150 mm x 4.6 mm, 5 µm two columns in series Reb A Stevioside 9
- Page 1 and 2: Hilic Columns for the Analysis of S
- Page 3 and 4: Hilic Hydrophilic Interaction Liqui
- Page 5 and 6: alculate Standard mixture the perce
- Page 7: 400 proved Standard method mixture
- Page 11 and 12: RP by Geuns Elution order Reb A Ste
- Page 13 and 14: Hilic ➔ solves Reb A / Stevioside
- Page 15 and 16: Hilic Columns Kinetex (Phenomenex)
- Page 17 and 18: Hilic Columns Kinetex (Phenomenex)
- Page 19 and 20: � � � � ���� Hilic
- Page 21 and 22: Stevia Leaf Extract dried and groun
- Page 23 and 24: Stevia Leave Stevioside Reb A UV MS
- Page 25 and 26: Hilic ➔ solves Reb A / Stevioside
- Page 27 and 28: ACN / Water 50 / 50 Water Hilic Sol
- Page 29 and 30: Conclusion ➔ Hilic columns are su
- Page 31 and 32: Minor Steviol Glycosides Ohta et al
- Page 33 and 34: Ohta et al., 2010 new 33
- Page 35: Conclusion ➔ Hilic columns are su
RP by Geuns<br />
Grace Alltima C18<br />
150 mm x 4.6 mm, 5 µm<br />
two columns in series<br />
Reb A<br />
Stevioside<br />
9
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