Hilic Columns for the Analysis of Steviol Glycosides - eustas en
Hilic Columns for the Analysis of Steviol Glycosides - eustas en Hilic Columns for the Analysis of Steviol Glycosides - eustas en
Hilic a (relatively) new stationary phase for HPLC Hydrophilic Interaction Liquid Chromatography Selectivity: orthogonal to RP (more or less) Gradient: 93 % acetonitrile ➔ 80 % acetonitrile Eluents: water, acetonitrile, additives: formic acid, ammonium formate … 4
alculate Standard mixture the percentage of total steviol glycosides (sum the nine rcentages). Column: Capcell pak C18 MG II 250 x 4.6 mm, 5 µm 8.0e-2 6.0e-2 4.0e-2 2.0e-2 2.35 3.42 Rebaudioside D 7.60 Rebaudioside A 8.08 Stevioside Rebaudioside F Rebaudioside C 9.72 Dulcoside A 10.72 11.73 Isocratic: 32:68 Acetonitrile:Water, pH 2.6 Flow: 1.0 mL/min JECFA, 2010 Rubusoside 15.62 23.23 21.57 0.0 Tim 0.00 5.00 10.00 15.00 20.00 25.00 30.00 Rebaudioside B Steviolbioside 5
- Page 1 and 2: Hilic Columns for the Analysis of S
- Page 3: Hilic Hydrophilic Interaction Liqui
- Page 7 and 8: 400 proved Standard method mixture
- Page 9 and 10: RP by Geuns Grace Alltima C18 150 m
- Page 11 and 12: RP by Geuns Elution order Reb A Ste
- Page 13 and 14: Hilic ➔ solves Reb A / Stevioside
- Page 15 and 16: Hilic Columns Kinetex (Phenomenex)
- Page 17 and 18: Hilic Columns Kinetex (Phenomenex)
- Page 19 and 20: � � � � ���� Hilic
- Page 21 and 22: Stevia Leaf Extract dried and groun
- Page 23 and 24: Stevia Leave Stevioside Reb A UV MS
- Page 25 and 26: Hilic ➔ solves Reb A / Stevioside
- Page 27 and 28: ACN / Water 50 / 50 Water Hilic Sol
- Page 29 and 30: Conclusion ➔ Hilic columns are su
- Page 31 and 32: Minor Steviol Glycosides Ohta et al
- Page 33 and 34: Ohta et al., 2010 new 33
- Page 35: Conclusion ➔ Hilic columns are su
<strong>Hilic</strong> a (relatively) new stationary phase <strong>for</strong> HPLC<br />
Hydrophilic<br />
Interaction<br />
Liquid<br />
Chromatography<br />
Selectivity: orthogonal to RP (more or less)<br />
Gradi<strong>en</strong>t: 93 % acetonitrile ➔ 80 % acetonitrile<br />
Elu<strong>en</strong>ts: water, acetonitrile, additives: <strong>for</strong>mic acid, ammonium <strong>for</strong>mate …<br />
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