METRA® CICP EIA Kit– Quick Guide C-terminal ... - Tecomedical

METRA ® CICP EIA Kit – Quick Guide
C-terminal Propeptide of Collagen Type I, also called PICP
Catalog No. 8003 (Quidel)
CEmark/FDA registered
The Metra CICP EIA kit provides a quantitative measure of the C-terminal
propeptide of type I collagen in serum. Collagen is synthesized as
procollagen. C- and N-terminal peptides are cleaved from the collagen
molecule prior to incorporation into the bone matrix and are released
into the circulation.
Elevated levels have been observed in diseases which are associated
with high bone turnover.
SAMPLE PREPARATION
Sample type: Serum, cell culture medium
Avoid haemolysis of specimens. Keep samples refrigerated at 2–8° C for
storage of less than 5 days. For longer storage freeze specimens at
-20° C or below.
NORMAL VALUES
Women 69-147 ng/mL
Men 76-163 ng/mL
Detailed normal ranges for children are available.
TECHNICAL SERVICE
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Switzerland +41 (0) 61 985 8100
France +33 (0) 143 671 276
Austria +43 (0) 180 101 2999
Benelux +31 (0) 33 495 1473
www.osteomedical.com
January 2006

METRA ® CICP EIA Kit – Quick Guide
ASSAY PROCEDURE
Dilute samples 1:12 with Assay Buffer Solution
Label frame with the required number of Assay Strips
Pipette 100 µl Standards, Controls, and diluted samples
Incubate 120 ± 5 minutes at 18 – 25° C.
Dissolve Enzyme Conjugate in
Wash Buffer, use within 2 h
Wash 3 times with 300 µl Wash Buffer
Add 50 µl Stop Solution.
Dilute Wash Buffer Concentrate
1:10 with dist. water
Add 100 µl Anti-CICP to each well
Incubate 45 - 50 minutes at 18 – 25°C
Add 100 µl Enzyme Conjugate to each well.
Incubate 45 - 50 minutes at 18 – 25°C
Prepare Substrate Solution 30-
60 minutes prior to use. Allow
tablets to dissolve completely.
Wash 3 times with 300 µl Wash Buffer
Wash 3 times with 300 µl Wash Buffer
Add 100 µl Substrate Solution to each well
Incubate 30 - 35 minutes at 18 – 25°C
Read Optical Density at 405 nm
(Reference measurement should be performed at 595 – 650 nm.)
Analyze the assay results
(Quantification software, 4-parameter fit: y = (A-D)/(1+(x/C)^B)+D )
January 2006