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Biologische Systeme und Medizin Poster: Mi., 14:00–16:30 M-P208
Digital in-line holography with synchrotron radiation
Axel Rosenhahn 1 , Ruth Barth 1 , Florian Staier 1 , Xinyu Cao 1 , Martina
Schürmann 1 , Stefan Eisebitt 2 , Michael Grunze 1
1 Angewandte Physikalische Chemie, Universität Heidelberg, INF 253, 69120 Heidelberg
– 2 BESSY m.b.H., Albert-Einstein-Str. 15, 12489 Berlin
Digital in-line holography has been proven to be a highly useful microscopy technique
that works without lenses and at the same time provides intrinsic three dimensional
information [1-3]. The principle of such a holographic microscope goes back to the initial
idea of D. Gabor of a ” new microscopic principle“ [4]. In this concept, coherent
radiation is scattered by an object and its interference with the unscattered wavefront
creates a diffraction pattern. As the unscattered background wave acts as reference,
each of these holograms intrinsically contains three dimensional information about the
investigated object. Both optically and nowadays digitally the scattering pattern can
be reconstructed and information about the investigated object retrieved in real space
[1]. In order to enhance the resolution of digital in-line holography it is straightforward
to consider a decrease of the wavelength. Therefore, we use photons in the XUV
region from 90 eV to about 350 eV, which includes the water window [5,6]. The feasibility
to use this wavelength range for coherent scattering experiments has previously
been proven [7,8,9]. We present results on XUV digital in-line holography using the
classical Gabor geometry with a highly divergent beam. As the production of highly
divergent XUV beams is a major challenge, possibilities and new point source designs
are shown that help to successfully suppress direct beam transmission in order to avoid
a beam block. To characterize the imaging properties, test samples with known properties
as polymer beads and lithographic structures were used to analyze resolution and
obtainable contrast. Additionally we show images of fibroblast cells to discuss future
biological perspectives of the technique. In order to make this new imaging technique
available to other users, the design of an experimental endstation exclusively dedicated
to holographic imaging will be described [10].
[1] W. Xu et al., Proc. Natl. Acad. Sci. USA 98 (2001) 11301
[2] J. Garcia-Sucerquia et al., Appl. Optics 45 (2006) 836
[3] J. Garcia-Sucerquia et al., Opt. Lett. 31 (2006) 1211
[4] D. Gabor, Nature 161 (1948) 777
[5] S. Vogt et al., J. Struct. Biology 132(2) (2000) 123
[6] W. Meyer-Ilse et al., J. Microscopy 201(3) (2001) 395
[7] J.E. Trebes et al., Science 238 (1987) 517
[8] R.A. Bartels et al., Science 297 (2002) 376
[9] S. Lindaas et al., J. Opt. Soc. Am. A 13(9) (1996) 1788
[10] BMBF Förderkennzeichen 05KS4VH1/5