Research Report Abstracts - Gesundheit

eS898
was maintained above 50% in females and 60% of men during
the study period. These values were significantly above
the baseline levels.
Conclusions: The strategic use of message banners effectively
encouraged physical activity among office worker over
an extended time period. Therefore, this intervention method
was found to successfully induce office workers to participate
in meaningful physical activity.
Implications: Message banners should be strategically
placed on walls in the workplace to promote a worker’s physical
activity. A message banner is an effective educational tool
for promoting occupational health.
Keywords: Occupational health; Physical activity; Daily
exercise
Funding acknowledgements: The authors thank the members
of the biochemistry laboratory of Kochi Women’s
University, Naomi Sano and Tadakatsu Inaoka for assisting
with this study, and the management of the site for their
assistance in conducting this survey.
Ethics approval: This study was reviewed and approved
by the ethics committee of the Kochi Women’s University,
Japan.
Research Report Poster Display
Number: RR-PO-201-9-Thu Thursday 23 June 12:00
RAI: Exhibit Halls 2&3
CARBON DIOXIDE-RICH WATER BATHING
INCREASES MYOGENIN PROTEIN EXPRESSION
IN RAT INJURED SKELETAL MUSCLE
Nonaka K. 1 , Mori J. 2 , Ito A. 2 , Tatsuta N. 2 , Akiyama J. 2 ,
Yonetsu R. 1 , Iwata A. 1
1Osaka Prefecture University, Faculty of Comprehensive
Rehabilitation, Osaka, Japan, 2Kibi International University,
Okayama, Japan
Purpose: Skeletal muscle exhibits a remarkable capacity for
regeneration after various injuries. However, muscle regeneration
has sometimes been found to be very slow. Because
fast repair of the injured muscle is the obvious target in sport
medicine, it is meaningful to find clinically feasible treatment
modalities that enhance the cell proliferation phase. Satellite
cells are responsible for the regeneration of adult skeletal
muscle tissues. Histopathological analysis of injured muscle
has shown increased numbers of satellite cells, and these
cells differentiate into myotubes and myofibers. Moreover,
Myogenic transcriptional factors, such as MyoD and Myogenin,
are key roles to regulate these processes and increases
in these protein levels are considered to promote the regeneration
of the injured skeletal muscle. Carbon dioxide-rich
water bathing has a long history and is known to increase
blood flow. Recently, carbon dioxide-rich water bathing was
reported to promote wound healing. We hypothesized that
carbon dioxide-rich water bathing would also promote the
regeneration of the injured skeletal muscle. The purpose of
this study was to investigate the effect of carbon dioxide-rich
water bathing on MyoD and Myogenin protein expression in
rat injured skeletal muscle.
Relevance: The findings could suggest the possibility to promote
the regeneration of the injured skeletal muscle.
Participants: Sixteen female Wistar rats, weighting
244–275 g, were used in this study.
Methods: The rats were assigned to a sedentary control
(Cont), bupivacaine-injected (BPVC), BPVC plus freshwater
bathing (BPVC + FW) and BPVC plus CO2-rich water
bathing (BPVC + CO2) (4 in each group). Left tibial anterior
(TA) muscle of rats in bupivacaine-injected groups were
injured by injection of 0.5% bupivacaine hydrochloride. Rats
in BPVC + FW and BPVC + CO2 were immersed into CO2rich
water (CO2 concentration, 1000 ppm) and freshwater,
respectively, for 30 minutes at 37 ◦ C once a day. After 4 days
of bupivacain hydrochloride treatment, Rats were killed and
left TA muscles were removed. MyoD and Myogenin protein
levels in removed TA muscles were measured by Western blot
analysis.
Analysis: Statistically analyses were performed by one way
ANOVA. When differences were observed, a host hoc Scheffe
test was performed. The level of statistical significance was
set at P < 0.05.
Results: MyoD was significant increased in BPVC,
BPVC + FW and BPVC + CO2 compared with Cont
(P < 0.01), but there was no significant difference between
BPVC, BPVC + FW and BPVC + CO2. Myogenin was significant
increased in BPVC, BPVC + FW and BPVC + CO2
compared with Cont (P < 0.01), and there was significant
increase in BPVC + CO2 compared with BPVC and
BPVC + FW (P < 0.05).
Conclusions: Myogenin is among myogenic transcriptional
factors that regulate myoblast proliferation or muscle growth.
Therefore, the increase in myogenin protein could induce to
promote the muscle regeneration. The results indicated that
carbon dioxide-rich bathing could promote the regeneration
of the injured skeletal muscle.
Implications: Carbon dioxide-rich water bathing could have
application as a physical therapy agency for treatment of the
injured skeletal muscle.
Keywords: Carbon dioxide-rich water bathing; Skeletal
muscle regeneration; Myogenic transcription factors
Funding acknowledgements: None.
Ethics approval: This study was approved the Animal Use
Committee at Kibi International University.