phage display protocols

phage display protocols
• Creative Biolabs develops and commercializes a full range of
innovative integrated services that are based on phage display
technology. We have extensive experience in library construction and
screening, antibody humanization, antibody affinity
maturation, production of specific monoclonal antibodies and much
more services using phage display techniques. We also provide
qualified antibody and peptide phage display libraries in-house to
assist our customers’ research and project development.

• Phage display is a kind of long-lasting laboratory platform for the
large-scale study of molecules interaction, such as protein-protein,
protein-peptide and protein-DNA interactions, and select molecules
with the highest affinity for specific targets. The technology was firstly
described by Smith (1985), and then be further expanded by such
groups of pioneers, e.g. Greg Winter and John McCafferty (Laboratory
of Molecular Biology), Richard Lerner and Carlos Barbas (Scripps
Research Institute), and Frank Breitling and Stefan Dübel (German
Cancer Research Center).

• The phage display technique relies on engineering bacteriophages to
display interested molecules (e.g. proteins and peptides) on their
surface. To achieve this, the gene encoding the interested molecule is
inserted into the gene of phage coat protein, which resulting in the
expression of interested molecule fused with the coated protein of
bacteriophage particles and a linkage between genotype and
phenotype. The phage display library is then built up by assembling
millions of genetically modified phages, which can be used for further
screening and selection. This platform can contribute to mimic
natural molecules within the cellular process and detect their
interaction with other molecules.

• With years of research and development, Creative Biolabs has
extensive experience in phage display platform by employing various
types of phage in various project objectives. In general, filamentous
bacteriophages (e.g. M13) are the most popular vehicle choice for
phage display and extensively used in many types of research.
Meanwhile, Creative Biolabs is also an expert to provide phage
display related services via either T4 or T7 phage systems, which can
provide different promising alternatives by their special merits.

• Phage display has been well-recognized as a pivotal tool in immunological and
cellular biological research due to its remarkable contribution to the
development of new drugs and vaccines. This technology has been widely used as
phage display library to select or isolate proteins, scaffolds, peptides or
antibodies with high affinity and specificity for interested targets.
• As one of the most successful applications, phage display technology has proved
its safety and efficiency in the research and development of specific antibodies.
Notably, take advantage of phage display platform based on human antibody
sequences makes it available to produce fully human antibodies without the
ethical and moral issues. In addition, this platform is also a wide choice for in
vitro protein evolution; a typical example is the well-known antibody affinity
maturation. Combine with mutagenesis and phage display technologies, phage
display antibody library with the size of over 10 8 (10 16 has ever been achieved
by Creative Biolabs) can be generated to screen the specific antibodies with high
affinity for the development of high efficacy with low side effects antibody drugs.