magic phage

magic phage
• Aiming at facilitating the progress of next-generation antibody therapies, Creative
Biolabs has integrated multiple state-of-the-art technologies and resources
during the past decades. We are proud to introduce our featured Magic Human
Antibody Discovery Platform to our worldwide customers. In particular, we
combine the genetically “humanized” mice with our strong expertise in phage
display immune library technology, offering a powerful approach for the
discovery of high specific, high-affinity antibody candidates.
• Phage display library construction and library screening has long been one of the
most widely used methods for antibody generation towards a variety of targets.
Conventional hybridoma technology may lose many potential binders during cell
fusion and screening process. In contrast, by creating a huge pool representing
the entire antibody repertoire, phage display technology allows the collection of
the whole set of antibodies in immunized animals, and subsequent isolation of
the best binders from the collection. Moreover, enriching steps and counter
selections can be easily incorporated in panning steps. Nowadays, it is
contributing to various biomedical research and industrial applications.

• Despite these appealing strengths of phage display technique, it is largely
restricted by immunization host - obviously one cannot immunize a human
for antibody development. In this regard, transgenic mice can offer a
promising solution. Transgenic mice, or “humanized” mice, have emerged
as one powerful toolbox for not only antibody development but also
pharmacokinetic/immunogenicity studies, and so on. They are constructed
through introducing human Ig transloci into mouse germline and silencing
endogenous mouse Ig gene at the same time. This way, these engineered
mice can then produce fully human antibodies. Creative Biolabs has
integrated several first-class transgenic mouse strains with diverse genetic
background, as well as different capacities to generate both normal (heavy
and light chain) antibodies and human sdAbs (heavy chain only). The
resulting antibody candidates can provide following advantages compared
to alternative methods:

• More robust discovery
• Optimal affinity, specificity, developability, and other properties (in
vivo selection)
• Fully human nature
• More potent functionalities
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In combination, phage display immune library technique can make the best
of the humanized mice for the discovery of ideal antibody binders against a
given target. We can obtain the entire antibody repertoire as a
comprehensive library, and perform high-throughput biopanning to get
high-affinity binders.

• As alternatives, Creative Biolabs also offers transgenic mouse/rat
hybridoma and Native plasma cell sorting strategies which can also
incorporate with transgenic mice for antibody development. Our
experienced scientists are always willing to discuss with every
customer on a case-to-case basis. Please feel free to inquiry us for
more details.
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