antibody library service
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<strong>antibody</strong> <strong>library</strong> <strong>service</strong><br />
• Creative Biolabs is one of the well-recognized experts who is<br />
professional in applying advanced phage display technologies for a<br />
broad range of project objectives. With years of experience, our<br />
scientists can offer high-quality phage display <strong>library</strong> construction and<br />
custom phage display <strong>library</strong> screening <strong>service</strong>s to meet our clients’<br />
demands precisely. Particularly, our <strong>service</strong>s also involve specific<br />
<strong>antibody</strong> discovery (e.g. PTM-specific <strong>antibody</strong>, anti-idiotype <strong>antibody</strong>,<br />
and agonistic <strong>antibody</strong>) and peptidome discovery.
• Phage display is one of the most powerful and widely used laboratory<br />
technique for the study of protein-protein, protein-peptide and<br />
protein-DNA interactions. This technology is mainly based on<br />
displaying the interest protein (peptides, antibodies, scaffolds or<br />
others) on the surface of employing phage and then be used to<br />
interrogate the constructed libraries containing millions or even<br />
billions of displayed phages. Theoretically, phage display is an<br />
exogenous gene expression method which the gene encoding the<br />
interest protein is inserted into bacteriophage coat protein gene then<br />
displaying the interest protein on the phage surfaces, resulting in a<br />
connection between genotype and phenotype.
• Due to the different properties and advantages, Creative Biolabs is<br />
pleased to tailor the most appropriate phage display system (M13, T4<br />
or T7) to meet our customers’ demands. Through DNA manipulation,<br />
numerous gene variants can be created and constructed as phage<br />
display <strong>library</strong>. Our scientists are confident in generating high-quality<br />
libraries with the diversity of 10 8 . Furthermore, to detect the<br />
interaction between displayed protein and those other molecules or<br />
isolate specific target binders, Creative Biolabs also utilizes the novel<br />
biopanning strategies to select high-affinity binders or ligands which<br />
are able to recognize the naïve targets.
• Antibody libraries are constructed by the genomic information coding<br />
for <strong>antibody</strong> variable domains, which can be derived from B cells of<br />
immune or naïve donors. Antibodies are the first proteins which were<br />
successfully displayed on the surface of phage by fusing the coding<br />
sequence of scFv or Fab to the coat protein. Due to the smaller size,<br />
scFv libraries are genetically more stable than Fab libraries, while Fab<br />
libraries lack the tendency to form higher molecular weight species,<br />
such as dimers and trimers, which can simplify the selection and<br />
characterization. Through binding the specific target, the interest<br />
<strong>antibody</strong> can then be eluted and retrieved.