phage library screening

phage library screening
• As a well-recognized expert at phage display technology, Creative
Biolabs offers our worldwide clients the best phage display library
screening services through our excellent biopanning strategies and stateof-the-art
equipment. Our scientists are pleased to tailor the most
appropriate strategies to screen the interested libraries of our customers.
• Acceptable Library Types
• Libraries from our customized library construction services
• In-house premade antibody libraries, peptide libraries, scaffold libraries,
etc.
• Libraries provided by our clients
• Other commercialized libraries

• According to different demands, a series of targets can be qualified
screened by our service. We accept targets provided by clients, but
also provide related services to synthesize and express the interested
targets.

• Acceptable Target Types
• Purified targets
• Unpurified targets
• Intact cells, tissues or any other in vivo targets
•
Phage display technology is one of the major approaches employed in various protein interaction studies,
especially in the discovery of specific antibodies, scaffolds or ligands. While binders with the desired
specificity generally exist at low frequencies in the constructed libraries. In this way, phage display library
screening has become an effective technique for the enrichment and identification of binders with high
affinity and specificity from interested libraries.
• At Creative Biolabs, the proprietary protocol and tailored biopanning strategy allow the increase of
candidates with higher affinity and desired specificity (e.g. binders recognize conformational epitope rather
than linear epitope). In general, by conducting four rounds library screening, our scientists can select
scFv/Fab antibodies with the affinity of 10 -7 . Through constructing serial sub-libraries of the isolated
scFv/Fab antibodies, we can increase the affinity from 10 -8 to 10 -9 . Moreover, Creative Biolabs has
successfully obtained a scFv antibody with an extremely high affinity of 10 -12 .
• Based on our standard biopanning protocol, Creative Biolabs can utilize different strategies for library
screening to meet our clients’ project goals.