scfv antibody library screening

scfv antibody library screening
• As a well-recognized expert at phage display technology, Creative Biolabs offers
our worldwide clients the best phage display library screening services through
our excellent biopanning strategies and state-of-the-art equipment. Our scientists
are pleased to tailor the most appropriate strategies to screen the interested
libraries of our customers.
• Acceptable Library Types
• Libraries from our customized library construction services
• In-house premade antibody libraries, peptide libraries, scaffold libraries, etc.
• Libraries provided by our clients
• Other commercialized libraries
•

• Acceptable Target Types
• Purified targets
• Unpurified targets
• Intact cells, tissues or any other in vivo targets
•
Phage display technology is one of the major approaches employed in various protein interaction studies,
especially in the discovery of specific antibodies, scaffolds or ligands. While binders with the desired
specificity generally exist at low frequencies in the constructed libraries. In this way, phage display library
screening has become an effective technique for the enrichment and identification of binders with high
affinity and specificity from interested libraries.
• At Creative Biolabs, the proprietary protocol and tailored biopanning strategy allow the increase of
candidates with higher affinity and desired specificity (e.g. binders recognize conformational epitope rather
than linear epitope). In general, by conducting four rounds library screening, our scientists can select
scFv/Fab antibodies with the affinity of 10 -7 . Through constructing serial sub-libraries of the isolated
scFv/Fab antibodies, we can increase the affinity from 10 -8 to 10 -9 . Moreover, Creative Biolabs has
successfully obtained a scFv antibody with an extremely high affinity of 10 -12 .
• Based on our standard biopanning protocol, Creative Biolabs can utilize different strategies for library
screening to meet our clients’ project goals.

Solid-Phase Screening : the most straightforward and widely used way to select binders against interested target adsorbed onto a solid surface. Typically, the targets can be coated on the well of i
Solution-Sorting Screening: an ideal approach to isolate binders recognize naïve targets. The target-binder interaction is carried out in solution with subsequent capture by the appropriate method
Cell-Based Screening: a method in particular for cell surface antigen selection. It is a reliable approach to screen cell surface antigens with native conformation, especially when the antigen is hard
In vivo Screening: a high throughput method for efficiently isolating tissue-specific binders. The selection is based on binding to target in vivo after the injection of interested phage libraries into who
Ex vivo Screening: a novel technique to employ primary cell suspensions of organs and tissues as targets. All cell types can be exposed for selection to identify the most specific binders.