Transcriptional regulation of meiosis in budding yeast

RIM15 A serine/threonine kinase whose activity is inhibited by PKA phosphorylation. Positive regulator
for the transcription of IME1 and for the interaction between Ime1 and Ume6. Phosphorylates
Ume6.
RME1 A Zn finger DNA-binding protein that represses the transcription of IME1 through the UCS4
element in IME1 promoter. The transcription of RME1 is negatively regulated by the Mata1/Matα2
complex.
ROX3 A component of the RNA polymerase SRB mediator complex. Required for repressing the
transcription of mid-late meiosis-specific genes.
RPD3 Histone deacetylase. Required for repression of EMG in media supporting vegetative growth.
Positive regulator of MMG.
SIN3 Negative regulator that is required for repression of EMG in media supporting vegetative growth.
Recruits Rpd3 to EMG due to its association with both Ume6 and Rpd3. Positive regulator of
MMG.
SIN4 A component of the RNA polymerase SRB mediator complex. Required for the repression activity
of Rme1, and for repressing the transcription of mid-late meiosis-specific genes.
SNF1 Serine/threonine protein kinase whose activity is inhibited by high levels of glucose. Required for
the transcription of both IME1 and IME2, and for spore formation.
SNF2 Component of the Swi/Snf transcriptional activation complex. Required for high-level expression
of IME1.
SOK2 Transcriptional repressor that binds SCB like sequences. Positive regulator in the mitotic cell cycle
Negative regulator of pseudohyphal growth, meiosis and IME1 transcription. Sok2 function as a
repressor depends on phosphorylation by PKA.
SSN6 A general transcriptional repressor that regulates the transcription of IME1. Forms a repression
complex with Tup1.
SUM1 A transcriptional repressor of MMG that binds the MSE* element. Recruits histone deacetylase
activity by association with Hst1.
SWI1 Component of the Swi/Snf transcriptional activation complex. Required for high-level expression
of IME1.
TPK1,2,3 Three genes encoding the catalytic subunit of PKA.
TUP1 A general transcriptional repressor that regulates the transcription of IME1. Forms a repression
complex with Ssn6.
UME2 A component of the SRB subcomplex of RNA polymerase II holoenzyme. Negative regulator of
EMG transcription.
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