Transcriptional regulation of meiosis in budding yeast

More documents

Recommendations

Info

Table 1. List of positive and negative regulators of transcription of meiosis-specific genes. Name Brief description of function ABF1 An essential DNA-binding protein required for DNA replication and the transcription of several meiosis-specific genes carrying its binding site (UASH). AMA1 A meiosis-specific subunit of APC/C that is required for degradation of CLB1 in the meiotic cycle. Required for the transcription of LMG. BCY1 The regulatory subunit of PKA. Positive regulator for IME1 transcription and sporulation. CDC25 Ras GDP/GTP exchange factor. Positive activator of PKA, negative regulator of IME1 transcription. Transmits the nitrogen signal to UCS1 and the glucose signal to IREu elements in IME1 promoter. CDC28 Cyclin dependent kinase that regulates initiation and progression in the mitotic cell cycle. In a complex with Cln phosphorylates Ime1 and sequesters it out of the nuclei. Required to activate Ime2 at the time cells enter the meiotic division. CDC35 Adenylate cyclase. Positive activator of PKA, negative regulator of IME1 transcription and sporulation GCN5 Histone acetylase required for the transcription of EMG. GPA2 A Gα protein that associates, when GTP bound, with Ime2. An inhibitor of Ime2 kinase activity. HST1 Histone deacetylase. Represses the transcription of MMG following recruitment by Sum1. IDS2 A positive regulator of Ime2 activity. IME1 The master regulator gene of meiosis. A transcriptional activator; recruited by Ume6 to the URS1 element in EMG, and is required for their transcription. The transcription of IME1 depends on the presence of Mata1 and Matα2 as well as on glucose and nitrogen depletion. Translation and activity of Ime1 are regulated by nutrients. IME2 An early meiosis-specific gene encoding serine/threonine kinase, homologous to the human CDK2. Required for timely and high level transcription of EMG, and for the transcription of MMG and LMG. Negative regulator of Ime1 stability. IME4 A positive transcriptional regulator of IME1 whose transcription depends on the presence of Mata1 and Matα2 and nitrogen depletion. ISW2 In a complex with Itc1 functions as an ATP dependent chromatin-remodeling factor. Required for the repression of EMG under vegetative growth conditions. Physically associate with Ume6 that recruits it to URS1 elements. ITC1 In a complex with Isw2 functions as an ATP dependent chromatin-remodeling factor. Required for the repression of EMG under vegetative growth conditions. 65
MCK1 Protein kinase required for efficient transcription of IME1, expression of EMG, MMG and spore maturation. Homologous to mammalian glycogen synthase kinase 3 and S. cerevisiae, RIM11, YOL128c, and MRK1. Required for phosphorylation of Ume6 and sporulation in cells deleted for RIM11 and MRK1. MDS3 Negative regulator of IME1 transcription. Redundant function with its homolog, PMD1 MSN2/4 Zinc finger transcriptional activators, which share redundant function and bind STRE elements. Negative regulators of mitosis. Positive regulators of meiosis and IME1 transcription through the IREu element in IME1 promoter. Targets of the cAMP/PKA pathway. MRK1 Protein kinase homologous to mammalian glycogen synthase kinase 3 and S. cerevisiae, RIM11, YOL128c, and MCK1.Required for phosphorylation of Ume6 and sporulation in cells deleted for RIM11 and MCK1. NDT80 A meiosis-specific transcriptional activator that binds the MSE element in MMG. An early MMG. PMD1 Negative regulator of IME1 transcription. Redundant function with its homolog, MDS3 PTP2,3 Tyrosine phosphatases, required for Mck1 dephosphorylation as well as for high-level transcription of IME1 and IME2, and for expression of MMG and LMG. RAS2 Small G protein that activates the cAMP/PKA and the MAPK signal pathways. Negative regulator of IME1 transcription. RES1 A dominant mutation in this gene bypasses MAT regulation for the transcription of IME1. RGR1 A component of the RNA polymerase SRB mediator complex. Required for the repression activity of Rme1. RIM1 A C2H2 zinc finger protein required for high-level expression of IME1, and efficient sporulation. Required for mitochondria DNA replication and maintenance. Activity is regulated by cleavage that depends on Rim8, Rim9, Rim13 and Rim20. RIM4 An early meiosis-specific gene required for high-level expression of EMG. Sequence identifies two RNA recognition motifs that are essential for function. RIM8 Positive regulator of IME1 transcription. Required for activating Rim1. RIM9 Positive regulator of IME1 transcription. Required for activating Rim1. RIM11 Tyrosine/serine/threonine kinase that is homologous to mammalian glycogen synthase kinase 3 and S. cerevisiae, MCK1, YOL128c, and MRK1.Required for the interaction between Ime1 and Ume6, and consequently for the transcription of EMG. Associates and phosphorylates both Ime1 and Ume6. RIM13 Positive regulator of IME1 transcription. Required for activating Rim1. RIM20 Positive regulator of IME1 transcription. Required for activating Rim1. 66
Page 1 and 2:
Transcriptional regulation of meios
Page 3 and 4:
3. Proteins required for the associ
Page 5 and 6:
I. Introduction The budding yeast S
Page 7 and 8:
et al., 2000). On the other hand, i
Page 9 and 10:
the UCS4 element upstream of the CY
Page 11 and 12:
egulator of Cyr1, CDC25 encodes the
Page 13 and 14:
1998). Furthermore, unlike IREu, IR
Page 15 and 16: In the absence of glucose Sok2 does
Page 17 and 18: autophosphorylation, including on a
Page 19 and 20: hours in SPM, followed by a decline
Page 21 and 22: 4. The SIN3, UME6, and RPD3 genes.
Page 23 and 24: 1993). Nevertheless, when Ime1 is t
Page 25 and 26: However, direct demonstration of ph
Page 27 and 28: 3. Proteins required for the associ
Page 29 and 30: heterologous promoter does not supp
Page 31 and 32: expressed only in the absence of gl
Page 33 and 34: structure of hCDK2 reveals that cyc
Page 35 and 36: terminal non-essential domain (Komi
Page 37 and 38: is dependent on Ime2, but in cells
Page 39 and 40: VI. Transcriptional regulation of l
Page 41 and 42: The transcriptional activator that
Page 43 and 44: Functional interaction between Ime1
Page 45 and 46: Bowdish, K. S., and Mitchell, A. P.
Page 47 and 48: Foiani, M., Nadjar-Boger, E., Capon
Page 49 and 50: Jeffrey, P. D., Russo, A. A., Polya
Page 51 and 52: Lindgren, A., Bungard, D., Pierce,
Page 53 and 54: Pazin, M. J., and Kadonaga, J. T. (
Page 55 and 56: Shenhar, G. (2001). TRANSCRIPTIONAL
Page 57 and 58: Szent-Gyorgyi, C. (1995). A biparti
Page 59 and 60: Yoshida, M., Kawaguchi, H., Sakata,
Page 61 and 62: Fig. 4. The function of positive an
Page 63 and 64: Fig. 9. A schematic structure of IM
Page 65: activation by Ime2 is due to phosph
Page 69: UME3 A cyclin C homolog that associ
show all

Transcriptional regulation of meiosis in budding yeast

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?