Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
VersaChrome Edge TM Tunable Filters<br />
TECHNICAL NOTE<br />
Tunable Edge Filters<br />
As fluorescence technology evolves, so too must the optical filters that are key<br />
TLP01-628<br />
TLP01-790 to detection. Almost every new fluorophore requires its own bandpass filter<br />
TSP01-628<br />
TSP01-790<br />
to yield the best brightness or contrast. That optimal bandpass may need to<br />
change in order to maximize signal to noise when used in conjunction with<br />
other fluorophores. The typical approach to designing a new optical system or<br />
developing a new test (new fluorophore, chemistry, etc.) is to try an assortment<br />
TLP01-561<br />
TLP01-704<br />
TSP01-561<br />
TSP01-704<br />
of our catalog bandpass filters that span the right wavelength range, testing and<br />
500 550 600 650 700 750 800 selecting the one that performs best in practice. While <strong>Semrock</strong>’s online plotting<br />
Wavelength (nm)<br />
and analysis tool SearchLight (searchlight.semrock.com) makes analysis and<br />
selection of the best prospective bandpass filters quick and easy, sometimes there is no perfect match to be found<br />
off the shelf.<br />
Until now, customers unable to find a catalog bandpass filter to meet their needs had to choose between using a<br />
suboptimal filter or purchasing a prototype run of a custom filter specification at significant cost. VersaChrome Edge<br />
tunable filters seek to fill that gap, allowing both researchers and instrument developers the ability to dynamically create<br />
and optimize their own bandpass filter shapes by combining three simple, versatile filters.<br />
100<br />
Cubes Laser<br />
Sets<br />
Full Spectrum<br />
Blocking Filter<br />
Wavelength (nm)<br />
+<br />
AOI: 0° – 60° AOI: 0° – 60°<br />
Tunable<br />
Long-pass Filter<br />
Wavelength (nm)<br />
+<br />
Tunable<br />
Short-pass Filter<br />
Wavelength (nm)<br />
=<br />
CWL and Bandwith<br />
Tunable bandpass filter<br />
Wavelength (nm)<br />
Transmission (%)<br />
90<br />
80<br />
70<br />
60<br />
60°<br />
0°<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
550 590 630 670 710 750 790 830 870 910 950<br />
Wavelength (nm)<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
60° 0°<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
550 590 630 670 710 750 790 830 870 910 950<br />
Wavelength (nm)<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
With the VersaChrome Edge Tunable Filters, <strong>Semrock</strong> is offering a new way to prototype. Our three new families of filters<br />
AOI=35° AOI=40° AOI=45° AOI=50° AOI=55° AOI=60°<br />
are designed to work together to create the equivalent of a single passband filter in the visible or near infrared. This allows<br />
researchers and instrument designers alike to not only create the bandpass they need, but also to fine-tune edge positions<br />
and passband width to maximize brightness and contrast/signal-to-noise in real time, within their measurement setup.<br />
AOI=0° AOI=5° AOI=10° AOI=15° AOI=20° AOI=25° AOI=30°<br />
VersaChrome Edge tunable filters now allow you to design and optimize that perfect match for yourself – not on paper,<br />
but in the lab. The principle is simple: combine a long-wave pass filter with a short-wave pass filter to create the bandpass<br />
shape, and place in tandem with a full-spectrum blocking filter to provide extended out-of-band blocking. Together, the<br />
three filters perform like a traditional bandpass filter. The true elegance in the solution, however, lies in the edge filters<br />
themselves. By using our VersaChrome Edge tunable long wave pass and short wave pass filters, each edge can be angletuned<br />
to the precise cut-on or cut-off wavelength you need. Extended blocking down to UV wavelengths (250 nm) and up<br />
to the near infrared (NIR) can be provided by the addition of a full spectrum blocking filter.<br />
The result is that, by combining angle-tuned TLP and TSP filters with a full-spectrum<br />
blocking filter, it is possible to create a passband filter with any center wavelength over<br />
a range of wavelengths in the visible and near infrared, and a passband width (FWHM)<br />
of any width from ≤ 5 nm to at least 12% of the CWL (~75 nm at 628 nm,<br />
or 120 nm at 1000 nm). Edge filters are available to independently control each edge<br />
to generate the exact passband required with edge wavelengths from 400 nm to<br />
beyond 1100 nm.<br />
Edge Type Passband edge at (nm) Use these filters Set at these AOIs<br />
LWP 727.5 TLP01-790 45.58°<br />
SWP 768.5 TLP01-790 29.57°<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
500 520 540 560 580 600 620 640 660 680 700<br />
Wavelength (nm)<br />
Input the desired Center-Wavelength (CWL) and Guaranteed-Minimum-Bandwidth (GMBW) or Full-Width-Half-Maximum<br />
(FWHM) to achieve a required passband using <strong>Semrock</strong>’s VersaChrome Edge Tunable Filter Calculator (see page 84)<br />
to calculate which filters and rotational angles will achieve this result.<br />
TLP01-628@60°<br />
TSP01-628@50°<br />
TLP01-628@40°<br />
TSP01-628@0°<br />
More<br />
82