Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets Cubes Laser Sets NLO Filters Individual Filters Dichroic Beamsplitters BrightLine ® Single-edge Laser Dichroic Beamsplitters UV & IR Laser Dichroic Beamsplitters (polarization-insensitive; for use at 45°) Nominal Edge Wavelength Property Value Comment Absolute Reflection > 98% (s-polarization) > 90% (p-polarization) > 94% (average polarization) Absolute reflectivity over the specified laser wavelengths/bands Average Reflection > 90% (average polarization) Averaged over extended reflection range Transmission > 93% Averaged over the transmission band above Angle of Incidence 45.0° Dependence of Wavelength on Angle of Incidence (Edge Shift) Cone Half Angle (for non-collimated light) Transmitted Wavefront Error Beam Deviation Laser Wavelengths 0.2% / degree Range for above optical specifications Based on a collimated beam of light Linear relationship valid between about 40°- 50° (See MyLight for actual performance) < 0.5° Rays uniformly distributed and centered at 45° < λ / 4 RMS at λ = 633 nm ≤ 10 arcseconds Peak-to-valley error < 5 x RMS value measured within clear aperture Second Surface Anti-reflection (AR) coated Flatness (Di01 & Di02) Reflection of a collimated, Gaussian laser beam with waist diameter up to 2.5 mm causes less than one Rayleigh Range of focal shift after the objective or a focusing lens. Filter Orientation Reflective coating side should face toward light source and sample (see page 27) Microscope Compatibility Extended Avg. Reflection Band Absolute Reflection Band Avg. Transmission Band Size (mm) (L x W x H) Filter Part Number Price 273 nm 266.0 nm 230.0 – 245.0 nm 245.0 – 266.0 nm 277.0 – 1200.0 nm 25.2 x 35.6 x 1.05 Di01-R266-25x36 $495 331 nm 325.0 nm 230.0 – 300.0 nm 300.0 – 325.0 nm 336.0 – 1200.0 nm 25.2 x 35.6 x 1.05 Di01-R325-25x36 $495 363 nm 355.0 nm 230.0 – 325.0 nm 325.0 – 355.0 nm 367.0 – 1200.0 nm 25.2 x 35.6 x 1.05 Di01-R355-25x36 $495 840 nm 993 nm 1078 nm 785.0 ± 5 nm 808.0 + 2 nm 830.0 nm 975.0 ± 5 nm 976.0 nm 980.0 nm 1030.0 nm 1047.1 nm 1064.0 nm BrightLine laser dichroic beamsplitters have extended reflection down to 350 nm to enable photoactivation. These dichroic beamsplitters are optimized for the most popular lasers used for fluorescence imaging, including all-solid-state lasers. Reflection is guaranteed to be > 98% (s-polarization) and > 94% (average polarization) at the laser wavelengths, plus > 93% average transmission and very low ripple over extremely wide passbands – out to 900 and even 1200 nm. 350.0 – 780.0 nm 780.0 – 830.0 nm 845.0 – 1600.0 nm 25.2 x 35.6 x 1.05 Di02-R830-25x36 $495 350.0 − 970.0 nm 970.0 − 980.0 nm 998.0 − 1600.0 nm 25.2 x 35.6 x 1.05 Di02-R980-25x36 $495 350.0 – 1030.0 nm 1030.0 – 1064.0 nm 1083.2 – 1600.0 nm 25.2 x 35.6 x 1.05 Di02-R1064-25x36 $495 Laser Dichroic Beamsplitters Common Specifications BrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes. All other mechanical and reliability specifications are the same as BrightLine dichroic specifications on page 34. For multiedge laser-optimized dichroic beamsplitters, see page 71 and 73 Tunable Filters More 72
BrightLine ® Laser Multiedge Dichroic Beamsplitters Laser Multiedge Dichroic Beamsplitters Nominal Edge Wavelength Laser Wavelengths (nm) Optimized for the most popular lasers used for fluorescence imaging, including all-solid-state lasers that are replacing older gas-laser technology. Laser Multiedge Dichroic Beamsplitters offer exceptionally high reflection at the laser wavelengths combined with very steep transitions from high reflection to high transmission (< 2.5% of the longest laser wavelength). They also offer sufficient flatness for laser applications (see Technical Note on page 67). Absolute Reflection Band (nm) Average Transmission Band (nm) 420 nm 375 ± 3, 405 ± 5 > 94% 370.0 – 410.0 > 93% 429.5 – 462.0 497 nm 473 +2/-0, 488 +3/-2 > 94% 473.0 – 491.0 > 93% 502.5 – 574.5 602 nm 593.5 , 594.1, 594 ± 0.3 > 94% 588.3 – 594.3 > 93% 612.0 – 800.0 497 nm 473 +2 /-2, 488 +3 /-2 > 94% 471.0 – 491.0 > 93% 503.5 – 526.5 552 nm 543 ± 1 > 94% 541.5 – 544.5 > 93% 560.0 – 615.5 656 nm 632.8, 635 +7/-0, 647.1 > 94% 632.8 – 647.1 > 93% 665.5 – 800.0 Super-resolution dual-edge and quad-edge dichroic beamsplitters, see page 71 Size (mm) (L x W x H) Filter Part Number Price 25.2 x 35.6 x 1.05 Di01- R405/488/594- 25x36 25.2 x 35.6 x 1.05 Di01- R488/543/635- 25x36 $515 $515 Fluorophores Single-band Sets Multiband Sets Cubes SEARCHLIGHT SearchLight allows fluorescence microscope users and optical instrument designers to predetermine the optimal fluorophore, light source, detector, and optical filter combinations for their microscope or system. By removing the guesswork and hours of searching multiple sources for spectral data, SearchLight users will be able to eliminate trial-and-error headaches and work more efficiently. Users may select from an extensive collection of preloaded spectra or upload their own spectral data in this free and openly accessible tool. Users can also save and share their data securely. Share: The share feature within SearchLight enables collaboration across researchers, engineers, companies and institutions by creating a unique URL link to the session which can be emailed to a colleague or collaborator. Laser Sets NLO Filters Use SearchLight now. Save time later. Try it at: http://searchlight.semrock.com Individual Filters Review <strong>Semrock</strong>’s article featured in BioOptics World Practical Considerations for Advanced Microscopy Our recent article in BioOptics World:Learn some of the key optics considerations that will help maximize image fidelity and quality, light transmission, and resolution. http://www.laserfocusworld.com/articles/print/volume-52/issue-10/issue-10/optics-advancedmicroscopy-practical-considerations-for-advanced-microscopy.html Dichroic Beamsplitters Tunable Filters 73 More
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Semrock 2018 Master Catalog Everyth
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Welcome to the 2018 Master Catalog
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T T T T Laser & Optical System Filt
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SearchLight Optimization Calculator
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The Semrock Advantage Proven Reliab
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BrightLine ® Single-band Sets When
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BrightLine ® Single-band Sets for
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BrightLine ® Multiband Sets for Po
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BrightLine ® Single-band Sets Filt
Page 19 and 20:
TECHNICAL NOTE Ultraviolet (UV) Flu
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BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73: BrightLine ® Super-resolution / TI
Page 77 and 78: LaserMUX TM Beam Combining Filters
Page 79 and 80: TECHNICAL NOTE Measurement of Optic
Page 81 and 82: VersaChrome ® Tunable Filters TECH
Page 83 and 84: VersaChrome ® Tunable Bandpass Fil
Page 85 and 86: VersaChrome Edge TM Tunable Filters
Page 87 and 88: Laser Wavelength Reference Table La
Page 89 and 90: Polarization Filters Common Specifi
Page 91 and 92: General Purpose Mirrors Semrock gen
Page 93 and 94: EdgeBasic TM Long / Short Wave Pass
Page 95 and 96: Edge Steepness and Transition Width
Page 97 and 98: RazorEdge ® Common Specifications
Page 99 and 100: Filter Types for Raman Spectroscopy
Page 101 and 102: MaxLine ® Laser-line Spectra and S
Page 103 and 104: Near Infrared Bandpass Filters Tran
Page 105 and 106: StopLine ® Single-notch Filter Com
Page 107 and 108: TECHNICAL NOTE Filter Spectra at No
Page 109 and 110: Working with Optical Density Optica
Page 111 and 112: LEARN MORE WITH OUR VIDEO COLLECTIO
Page 113 and 114: The Physics of Pixel Shift Pixel sh
Page 115 and 116: Get the Right Solution, Right Now W
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