Semrock Master Catalog 2018

Fluorophores
Single-band
Sets
BrightLine ® Super-resolution / TIRF Laser
Dichroic Beamsplitters
TECHNICAL NOTE
Finding the Right Dichroic Beamsplitter
Semrock makes a wide variety of 45º dichroic beamsplitters optimized for different purposes. Every dichroic utilizes our
advanced hard, ion-beam-sputtered coating technology for exceptional environmental and handling durability and no
degradation even under the most intense illumination conditions. The dichroics are broadly categorized by the light source
with which they are intended be used and the spectral edge steepness and physical flatness values required for various
applications. The table below lists six broad families of Semrock dichroic beamsplitters according to these requirements.
Multiband
Sets
Light Source Edge Steepness Flatness / RWE
Family
Page
Classification
Broadband Standard Standard Epi-fluorescence General Purpose Dichroics 62
Broadband Standard Image-splitting Image-splitting Dichroics 64
Laser lines Steep Laser Laser Dichroics 72
Laser lines Steep Super-resolution / TIRF Super-resolution / TIRF Dichroics 71
Laser lines Standard Laser Laser Notch Dichroics 74
Cubes Laser
Sets
NLO
Filters
Laser lines Standard Laser Laser Beam Combining 75
Laser lines Standard Super-resolution / TIRF Multiphoton Laser Beam Combining 45
Precise laser lines Ultrasteep Laser Ultrasteep Laser Dichroics 96
Dichroic beamsplitters designed to be used with broadband light sources generally ensure the highest average value of reflection
over a band of source wavelengths often chosen for best overlap with a particular fluorophore absorption spectrum. Dichroics for
laser light sources ensure high absolute reflection performance at specified laser lines, with precise spectral edges that are keyed
to these lines and anti-reflection (AR) coatings on the filter backsides to minimize any coherent interference artifacts.
While all Semrock dichroics are among the steepest available 45º edge filters on the market, those optimized for
laser-based epifluorescence and Raman applications are exceptionally steep to enable signal collection as close
as possible to the laser line.
Flatter dichroic beamsplitters minimize wavefront errors that can result in defocus and imaging aberrations of the light reflected
off these filters. Semrock classifies dichroic beamsplitters into four categories of flatness, as listed in the table below.
NOTE: Mounting can impact flatness performance. Values below apply to unmounted parts.
Flatness / RWE Classification & Application Specification of Semrock Dichroic Beamsplitters
Individual
Filters
Dichroic
Beamsplitters
Flatness / RWE
Classification
Nominal Radius
of Curvature
Application Specification
All Classifications All curvatures Transmission: does not cause signficant aberrations to a transmitted beam over the full Clear Aperture
Super-resolution
/ TIRF
~ 1275 meters Reflection: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror)
at the focal plane of a lens after reflecting a laser beam with a diameter up to 22.5 mm
~ 255 meters Reflection: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror)
at the focal plane of a lens after reflecting a laser beam with a diameter up to 10 mm
Image-splitting ~ 1275 meters Reflection: contributes less than 1.5 x Airy Disk diameter to the RMS spot size of a focused, reflected
emission beam with a diameter up to 37 mm
~ 100 meters Reflection: contributes less than 1.5 x Airy Disk diameter to the RMS spot size of a focused, reflected
emission beam with a diameter up to 10 mm
Tunable
Filters
Laser ~ 30 meters Reflection: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror)
at the focal plane of a lens after reflecting a laser beam with a diameter up to 2.5 mm
Standard Epifluorescence
~ 6 meters Reflection: designed to reflect broadband excitation light that is not focused or imaged
More
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