Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets BrightLine ® Image-splitting Dichroic Beamsplitters Image-splitting Dichroic Beamsplitters Nominal Edge Wavelength 484 nm Common Fluorophore Pairs to Split DAPI/FITC (or BFP/GFP) These beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multiple images. For applications such as FRET and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems (see Technical Note on page 67). Average Reflection Band Average Transmission Band Size (L x W x H) Filter Part Number Price 350 – 475 nm 492.3 – 950 nm 25.2 x 35.6 x 1.05 mm FF484-FDi01-25x36 $335 509 nm CFP/YFP 350 – 500 nm 518.3 – 950 nm 25.2 x 35.6 x 1.05 mm FF509-FDi01-25x36 $335 538 nm GFP/mOrange 350 – 528.4 nm 547.7 – 950 nm 25.2 x 35.6 x 1.05 mm FF538-FDi01-25x36 $335 560 nm YFP/dTomato 350 – 550 nm 570.1 – 950 nm 25.2 x 35.6 x 1.05 mm FF560-FDi01-25x36 $335 Cubes Laser Sets 580 nm GFP/mCherry (or FITC/TxRed) 350 – 570 nm 590.8 – 950 nm 25.2 x 35.6 x 1.05 mm FF580-FDi01-25x36 $335 640 nm Cy3/Cy5 350 – 629.5 nm 652 – 950 nm 25.2 x 35.6 x 1.05 mm FF640-FDi01-25x36 $335 662 nm TxRed/Cy5 350 – 650 nm 673.7 – 950 nm 25.2 x 35.6 x 1.05 mm FF662-FDi01-25x36 $335 Image-splitting Dichroic Beamsplitters for Super-resolution Microscopy Nominal Edge Wavelength Common Fluorophore Pairs to Split Average Reflection Band Average Transmission Band Size (L x W x H) Filter Part Number Price New 509 nm CFP/YFP 350 – 500 nm 518.3 – 1200 nm 25.2 x 35.6 x 3.0 mm FF509-FDi02-t3-25x36 $545 NLO Filters New New 560 nm YFP/dTomato 350 – 550 nm 570.1 – 1200 nm 25.2 x 35.6 x 3.0 mm FF560-FDi02-t3-25x36 $545 580 nm GFP/mCherry 350 – 570 nm 590.8 – 1200 nm 25.2 x 35.6 x 3.0 mm FF580-FDi02-t3-25x36 $545 New 640 nm Cy3/Cy5 350 – 629 nm 652 – 1200 nm 25.2 x 35.6 x 3.0 mm FF640-FDi02-t3-25x36 $545 Individual Filters Image Splitting Dichroic Beamsplitters Common Specifications Property Value Comment Transmission > 93% Averaged over the specified band Reflection > 95% Averaged over the specified band Dichroic Beamsplitters Flatness (FFxxx-FDi01) Flatness (FFxxx-FDi02-t3) < λ/4 P-V at λ = 633 nm < λ/5 P-V RWE at λ = 633 nm Spherical error measured over a 10 mm aperture [1] Over Clear Aperture [1] A 10 mm spot size is typical assuming common microscope values. See www.semrock.com. All other mechanical specifications are the same as BrightLine dichroic specifications on page 34. Tunable Filters See spectra graphs and ASCII data for these filter sets at www.semrock.com More 64
BrightLine ® Multiedge Dichroic Beamsplitters Our BrightLine multiedge dichroic beamsplitters are available in dual, triple, quad, and the world’s only penta band designs. Optimized for general broadband excitation sources or laser lines, high performance, multi-color fluorescence imaging is easily attainable with <strong>Semrock</strong>’s BrightLine dichroic beamsplitters. Fluorophores Dual-edge General Purpose Dichroic Beamsplitters (polarization-insensitive; for use at 45°) For multiedge laser-optimized fluorescence dichroic beamsplitters, see page 71. Nominal Edge Wavelength 403 nm 502 nm 440 nm 520 nm 493 nm 574 nm 495 nm 605 nm 505 nm 606 nm 545 nm 650 nm 560 nm 659 nm Avg. Reflection Bands > 97.5% 370 – 393 nm > 97.5% 466 – 495 nm > 95% 415 – 432 nm > 95% 493 – 511 nm > 95% 456 – 480 nm > 95% 541 – 565 nm > 95% 454 – 485 nm > 95% 570 – 598 nm > 95% 458 – 499 nm > 95% 570 – 600 nm > 95% 532.0 nm > 95% 632.8 nm > 95% 514 – 553 nm > 95% 617 – 652 nm Avg. Transmission Bands > 90% 414 – 452 nm > 90% 510 – 550 nm > 90% 449 – 483 nm > 90% 530 – 569 nm > 90% 500 – 529 nm > 90% 584 – 679 nm > 90% 505 – 550 nm > 90% 620 – 675 nm > 90% 509 – 541 nm > 90% 612 – 647 nm > 90% 554 – 613 nm > 90% 658 – 742 nm > 90% 564 – 591 nm > 90% 665 – 718 nm Size (L x W x H ) Filter Part Number Price 25.2 x 35.6 x 1.05 mm FF403/502-Di01-25x36 $365 25.2 x 35.6 x 1.05 mm FF440/520-Di01-25x36 $365 25.2 x 35.6 x 1.05 mm FF493/574-Di01-25x36 $365 25.2 x 35.6 x 1.05 mm FF495/605-Di01-25x36 $365 25.2 x 35.6 x 1.1 mm FF505/606-Di01-25x36 $365 25.2 x 35.6 x 1.05 mm FF545/650-Di01-25x36 $365 25.2 x 35.6 x 1.05 mm FF560/659-Di01-25x36 $365 Single-band Sets Multiband Sets Cubes ACTUAL MEASURED DATA Transmission (%) 100 90 80 70 60 50 40 30 20 10 0 350 450 550 650 750 850 Wavelength (nm) Image-splitting dichroic beamsplitters spectra FF484-FDi01 FF509-FDi01 FF538-FDi01 FF560-FDi01 FF580-FDi01 FF640-FDi01 FF662-FDi01 950 Laser Sets Tunable Filters NLO Filters Individual Filters Dichroic Beamsplitters 65 More
Page 1 and 2:
Semrock 2018 Master Catalog Everyth
Page 3 and 4:
Welcome to the 2018 Master Catalog
Page 5 and 6:
T T T T Laser & Optical System Filt
Page 7 and 8:
SearchLight Optimization Calculator
Page 9 and 10:
The Semrock Advantage Proven Reliab
Page 11 and 12:
BrightLine ® Single-band Sets When
Page 13 and 14:
BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65: BrightLine ® Single-edge Dichroic
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
Page 77 and 78: LaserMUX TM Beam Combining Filters
Page 79 and 80: TECHNICAL NOTE Measurement of Optic
Page 81 and 82: VersaChrome ® Tunable Filters TECH
Page 83 and 84: VersaChrome ® Tunable Bandpass Fil
Page 85 and 86: VersaChrome Edge TM Tunable Filters
Page 87 and 88: Laser Wavelength Reference Table La
Page 89 and 90: Polarization Filters Common Specifi
Page 91 and 92: General Purpose Mirrors Semrock gen
Page 93 and 94: EdgeBasic TM Long / Short Wave Pass
Page 95 and 96: Edge Steepness and Transition Width
Page 97 and 98: RazorEdge ® Common Specifications
Page 99 and 100: Filter Types for Raman Spectroscopy
Page 101 and 102: MaxLine ® Laser-line Spectra and S
Page 103 and 104: Near Infrared Bandpass Filters Tran
Page 105 and 106: StopLine ® Single-notch Filter Com
Page 107 and 108: TECHNICAL NOTE Filter Spectra at No
Page 109 and 110: Working with Optical Density Optica
Page 111 and 112: LEARN MORE WITH OUR VIDEO COLLECTIO
Page 113 and 114: The Physics of Pixel Shift Pixel sh
Page 115 and 116: Get the Right Solution, Right Now W
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Semrock Master Catalog 2018

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