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Semrock Master Catalog 2018

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Fluorophores<br />

Single-band<br />

Sets<br />

Multiband<br />

Sets<br />

Cubes Laser<br />

Sets<br />

NLO<br />

Filters<br />

TECHNICAL NOTE<br />

You Can Clean <strong>Semrock</strong> Optical Filters!<br />

<strong>Semrock</strong> manufactures the most durable optical filters available.<br />

However, it is important to note that while all optical components<br />

should be handled with care, soft-coated filters are especially<br />

susceptible to damage by handling and cleaning. Fortunately,<br />

<strong>Semrock</strong> supplies only hard-coated filters, so all of <strong>Semrock</strong>’s filters<br />

may be readily cleaned using the following recommended method.<br />

The following are recommended to properly clean your filters:<br />

• Unpowdered laboratory gloves – prevent finger oils from<br />

contaminating the glass and keep solvents from contacting skin;<br />

• Eye protection – critical for avoiding getting any solvent in your eyes;<br />

• Compressed air – clean, filtered laboratory compressed nitrogen<br />

or air is ideal, but “canned” compressed air or even a rubber “bulb<br />

blower” in a relatively clean environment is acceptable;<br />

• Lint-free swab – cotton-based swabs work best;<br />

• Lens cleaning tissue – lint-free tissue paper is also acceptable;<br />

• Cleaning solvent – we recommend Isopropyl Alcohol (IPA)<br />

and/or Acetone. Care should be taken when handling these<br />

solvents, especially to avoid ingestion.<br />

1. Blow off contaminants. Many contaminants are loosely attached to<br />

the surface and can be blown off. Using laboratory gloves, hold the<br />

filter in one hand and aim the air stream away from the filter. Start<br />

the air stream using a moderate air flow. Maintaining an oblique<br />

angle to the part – never blow straight on the filter surface – now<br />

bring the air stream to the filter, and slowly move it across the<br />

surface. Repeat until no more loose particles are disappearing.<br />

2. Clean filter. If dust or debris remains, it is probably “stuck” to<br />

the surface and must be removed with mechanical force and/or<br />

chemical action. Create a firm but “pointy” tip with the lint-free<br />

wipe or lens tissue by folding it multiple times into a triangular<br />

shape or wrapping it around a swab. Lint-free swabs may also be<br />

used directly in place of a folded wipe. Moisten the wipe or swab<br />

with either IPA or Acetone, but avoid too much excess solvent.<br />

The key to cleaning<br />

the optic is to maintain<br />

one continuous motion<br />

at as constant a speed<br />

as possible. Some<br />

people prefer to<br />

clean using a “figure<br />

8” pattern while others<br />

choose to start in the<br />

center of the part and wipe<br />

outward in a spiral pattern.<br />

Do not stop the wipe on<br />

the surface – keep the wipe<br />

moving at a constant speed, lifting the moving wipe off the part<br />

when you reach the end of the pattern.<br />

3. Inspect filter. Use a room light or any bright light source to<br />

inspect the optic to ensure that it is clean. Tip, tilt, and rotate<br />

the optic while viewing it as close to your eye as you can focus. If<br />

contamination remains, start with a brand new wipe or swab and<br />

repeat step 2 above.<br />

4. Repeat steps 1 – 3 for the other side of the filter if<br />

contamination exists.<br />

Precautions for Edge Blackened Filters<br />

For <strong>Semrock</strong> edge blackened filters, the above procedures<br />

can be used with the following precautions.<br />

• Only IPA or water based cleaning solutions can be used.<br />

• Acetone, Methanol, and other chemical solutions should be<br />

avoided as they will damage the edge blackening material.<br />

• Aggressive wiping of the blackened edge should be avoided.<br />

Note: IPA and Acetone each have pros and cons, so choose the solvent that<br />

works best for you after trying both.<br />

Watch the video tutorial on how to clean<br />

your optical filters at www.semrock.com<br />

Individual<br />

Filters<br />

SEARCHLIGHT<br />

Dichroic<br />

Beamsplitters<br />

Tunable<br />

Filters<br />

SearchLight allows fluorescence microscope users and optical instrument designers to predetermine the optimal fluorophore,<br />

light source, detector, and optical filter combinations for their microscope or system. By removing the guesswork and hours of<br />

searching multiple sources for spectral data, SearchLight users will be able to eliminate trial-and-error headaches and work more<br />

efficiently. Users may select from an extensive collection of preloaded spectra or upload their own spectral data in this free and<br />

openly accessible tool. Users can also save and share their data securely.<br />

Share: The share feature within SearchLight<br />

enables collaboration across researchers,<br />

engineers, companies and institutions by<br />

creating a unique URL link to the session which<br />

can be emailed to a colleague or collaborator.<br />

Use SearchLight now. Save time later. Try it at:<br />

http://searchlight.semrock.com<br />

More<br />

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