Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
BrightLine ® Laser Fluorescence Multiband Sets<br />
Full Multiband Filter Sets - multiband exciter, emitter and beamsplitter<br />
Set / Laser Lines<br />
Primary Fluorophores<br />
Multiband<br />
Excitation<br />
(CWL/BW)<br />
Multiband<br />
Emission<br />
(CWL/BW)<br />
Multiedge<br />
Dichroic<br />
(Laser)<br />
Filter Set Part Numbers<br />
Base<br />
Price<br />
Fluorophores<br />
LF488/561-B (Dual-Band Full Multiband) LF488/561-B-000 $1195<br />
Green: FITC (Fluorescein), GFP (EGFP)<br />
Red: mCherry (RFP)<br />
482/18<br />
563/9<br />
523/40<br />
610/52<br />
488 nm<br />
561 nm<br />
LF405/488/594-A (Triple-Band Full Multiband) LF405/488/594-A-000 $1325<br />
Blue: DAPI, BFP (EBFP)<br />
Green: FITC (Fluorescein), GFP (EGFP)<br />
Red: mCherry, Texas Red ® 390/40<br />
482/18<br />
587/15<br />
446/32<br />
532/58<br />
646/68<br />
405 nm<br />
488 nm<br />
594 nm<br />
LF405/488/532/635-B (Quad-Band Full Multiband) LF405/488/532/635-B-000 $1545<br />
Blue: DAPI<br />
390/40 446/32 405 nm<br />
Green: FITC (Fluorescein), GFP (EGFP)<br />
482/18 510/16 488 nm<br />
Orange: TRITC<br />
532/3 581/63 532 nm<br />
Red: Cy5 TM 640/14 703/80 635 nm<br />
LF405/488/561/635-B (Quad-Band Full Multiband) LF405/488/561/635-B-000 $1545<br />
Blue: DAPI, BFP (EBFP)<br />
390/40 446/32 405 nm<br />
Green: FITC (Fluorescein), GFP (EGFP)<br />
482/18 523/42 488 nm<br />
Orange: mCherry (RFP)<br />
563/9 600/35 561 nm<br />
Red: Cy5 640/14<br />
TM 677/27 635 nm<br />
Filter Specifications on page 34<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Cubes<br />
See spectra graphs and ASCII data for these filter sets at<br />
www.semrock.com/FullMultiBand-Laser.aspx<br />
CUBES PAGE 35<br />
TECHNICAL NOTE<br />
Laser<br />
Sets<br />
TIRF using 1λ RWE Super-resolution Microscopy Cubes<br />
Super-resolution Microscopy Cubes set the new standard for laser based microscopes. These cubes are optimized for mounting<br />
1λ RWE 1 mm thick super-resolution laser dichroic beamsplitters. Maximize SNR and minimize artifacts in TIRF, confocal, PALM,<br />
STORM, SIM, and other super-resolution techniques.<br />
Conventional microscopy cubes can significantly compromise the flatness of the dichroic beamsplitters thereby introducing<br />
aberrations. But super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest<br />
quality components for best instrument sensitivity. Our industry-leading 1λ RWE 1 mm thick laser dichroic beamsplitters minimize<br />
focus shift and aberrations in the reflected beam compared to standard dichroic beamsplitters. However, in order to realize their<br />
full flatness potential, these dichroic beamsplitters need to be carefully mounted in microscopy cubes. <strong>Semrock</strong> has developed<br />
proprietary methods of installing 1λ RWE super-resolution 1 mm thick dichroic beamsplitters in cubes that guarantee the flatness<br />
performance. Offered as standard catalog products, cubes compatible with popular microscopes are available. 1λ RWE 1 mm<br />
dichroics installed in standard microscopy cubes eliminate the need to re-align a 2 mm thick dichroic.<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
“The new TIRF & superresolution<br />
microscopy<br />
cubes from <strong>Semrock</strong><br />
definitely provide more<br />
homogeneous and<br />
flat illumination in all<br />
excitation/emission bands,<br />
compared to other cubes<br />
I have seen so far”<br />
– Dr. Peker Milas, Ross Lab,<br />
University of Massachusetts<br />
Amherst<br />
Xenopus laevis embryos transfected with GFP-cadherin<br />
and RFP-actin imaged with TIRF using <strong>Semrock</strong>’s Di03-<br />
R405/488/561/635-t1 super-resolution dichroic in a full-multiband<br />
configuration. Image courtesy of Keck Imaging Center,<br />
University of Virginia.<br />
Actin filaments imaged in TIRF using <strong>Semrock</strong>’s Di03-<br />
R405/488/532/635-t1 super-resolution dichroic in a full-multiband<br />
configuration. Image courtesy of Ross Lab, University<br />
of Massachusetts Amherst<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
39<br />
More