Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets BrightLine ® Laser Fluorescence Single-band and Longpass Filter Sets Super-resolution / TIRF filter sets guarantee 1 λ P-V RWE filter set performance Filter wavelengths precisely keyed to popular laser lines, with steep transitions from laser blocking to fluorescence transmission Minimal reflected wavefront distortion for even large diameter illumination beams Exceptionally high transmission to maximize system throughput, thus reducing acquisition time Deep blocking at laser wavelengths to minimize noise background Designed for the unique demands of TIRF laser excitation, the dichroic beamsplitter reflection range extends down to 350 nm allowing the combined use of photoactivation, UV sources with the normal excitation laser line. Users of uncaging and super-resolution techniques will appreciate this added functionality. Maximize SNR and minimize artifacts in TIRF, confocal, PALM, STORM, SIM, and other super-resolution techniques. Longpass sets allow for longer wavelengths to be detected and more light to be captured INDICATES SUPER- RESOLUTION / TIRF Cubes Laser Sets NLO Filters Individual Filters Dichroic Beamsplitters NOTE: BrightLine Laser Fluorescence filter sets are optimized for laser excitation and inherently provide excellent image registration performance – when interchanging these sets with one another, minimal pixel shift is observed. Note that the laser filter sets are not designed to exhibit “zero pixel shift” performance when interchanging with BrightLine –ZERO TM filter sets. Images obtained with the laser filter sets exhibit excellent image registration not only with one another, but also with images obtained when no fluorescence filters are present (e.g. in DIC or other bright-field modes). Filter Set / Primary Fluorophores LF405/LP-C (Longpass) 375 & 405 nm LF405-C 375 & 405 nm LF442-C ~ 440 & 441.6 nm LF488/LP-D (Longpass) 473 & 488 nm LF488-D 473 & 488 nm LF514-C 514.5 & 515.0 nm LF561/LP-D 559, 561.4, & 568.2 nm LF561-C 559, 561.4, & 568.2 nm LF594/LP-D (Longpass) 593.5,594, 594.1 nm LF594-D 593.5, 594, 594.1 nm LF635/LP-C (Longpass) 632.8, 635, & 647.1 nm LF635-C 632.8, 635, & 647.1 nm Excitation (CWL/BW) Emission (CWL/BW) Dichroic (Laser) Filter Set Part Numbers Base Price 390/40 405/LP 405 nm LF405/LP-C-000 $965 390/40 452/45 405 nm LF405-C-000 $965 448/20 482/25 442 nm LF442-C-000 $965 482/18 488/LP 488 nm LF488/LP-D-000 $965 482/18 525/45 488 nm LF488-D-000 $965 510/10 542/27 514 nm LF514-C-000 $965 561/14 561/LP 561 nm LF561/LP-D-000 $965 561/14 609/54 561 nm LF561-C-000 $965 591/6 594/LP 594 nm LF594/LP-D-000 $965 591/6 647/57 594 nm LF594-D-000 $965 640/14 635/LP 635 nm LF635/LP-C-000 $965 640/14 676/29 635 nm LF635-C-000 $965 Filter Specifications on page 34 Tunable Filters See spectra graphs and ASCII data for these filter sets at www.semrock.com/SingleBand-Laser.aspx www.semrock.com/Longpass-Laser.aspx CUBES PAGE 35 More 38
BrightLine ® Laser Fluorescence Multiband Sets Full Multiband Filter Sets - multiband exciter, emitter and beamsplitter Set / Laser Lines Primary Fluorophores Multiband Excitation (CWL/BW) Multiband Emission (CWL/BW) Multiedge Dichroic (Laser) Filter Set Part Numbers Base Price Fluorophores LF488/561-B (Dual-Band Full Multiband) LF488/561-B-000 $1195 Green: FITC (Fluorescein), GFP (EGFP) Red: mCherry (RFP) 482/18 563/9 523/40 610/52 488 nm 561 nm LF405/488/594-A (Triple-Band Full Multiband) LF405/488/594-A-000 $1325 Blue: DAPI, BFP (EBFP) Green: FITC (Fluorescein), GFP (EGFP) Red: mCherry, Texas Red ® 390/40 482/18 587/15 446/32 532/58 646/68 405 nm 488 nm 594 nm LF405/488/532/635-B (Quad-Band Full Multiband) LF405/488/532/635-B-000 $1545 Blue: DAPI 390/40 446/32 405 nm Green: FITC (Fluorescein), GFP (EGFP) 482/18 510/16 488 nm Orange: TRITC 532/3 581/63 532 nm Red: Cy5 TM 640/14 703/80 635 nm LF405/488/561/635-B (Quad-Band Full Multiband) LF405/488/561/635-B-000 $1545 Blue: DAPI, BFP (EBFP) 390/40 446/32 405 nm Green: FITC (Fluorescein), GFP (EGFP) 482/18 523/42 488 nm Orange: mCherry (RFP) 563/9 600/35 561 nm Red: Cy5 640/14 TM 677/27 635 nm Filter Specifications on page 34 Single-band Sets Multiband Sets Cubes See spectra graphs and ASCII data for these filter sets at www.semrock.com/FullMultiBand-Laser.aspx CUBES PAGE 35 TECHNICAL NOTE Laser Sets TIRF using 1λ RWE Super-resolution Microscopy Cubes Super-resolution Microscopy Cubes set the new standard for laser based microscopes. These cubes are optimized for mounting 1λ RWE 1 mm thick super-resolution laser dichroic beamsplitters. Maximize SNR and minimize artifacts in TIRF, confocal, PALM, STORM, SIM, and other super-resolution techniques. Conventional microscopy cubes can significantly compromise the flatness of the dichroic beamsplitters thereby introducing aberrations. But super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components for best instrument sensitivity. Our industry-leading 1λ RWE 1 mm thick laser dichroic beamsplitters minimize focus shift and aberrations in the reflected beam compared to standard dichroic beamsplitters. However, in order to realize their full flatness potential, these dichroic beamsplitters need to be carefully mounted in microscopy cubes. Semrock has developed proprietary methods of installing 1λ RWE super-resolution 1 mm thick dichroic beamsplitters in cubes that guarantee the flatness performance. Offered as standard catalog products, cubes compatible with popular microscopes are available. 1λ RWE 1 mm dichroics installed in standard microscopy cubes eliminate the need to re-align a 2 mm thick dichroic. NLO Filters Individual Filters “The new TIRF & superresolution microscopy cubes from Semrock definitely provide more homogeneous and flat illumination in all excitation/emission bands, compared to other cubes I have seen so far” – Dr. Peker Milas, Ross Lab, University of Massachusetts Amherst Xenopus laevis embryos transfected with GFP-cadherin and RFP-actin imaged with TIRF using Semrock’s Di03- R405/488/561/635-t1 super-resolution dichroic in a full-multiband configuration. Image courtesy of Keck Imaging Center, University of Virginia. Actin filaments imaged in TIRF using Semrock’s Di03- R405/488/532/635-t1 super-resolution dichroic in a full-multiband configuration. Image courtesy of Ross Lab, University of Massachusetts Amherst Dichroic Beamsplitters Tunable Filters 39 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39: BrightLine ® Laser Filter and Set
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
Page 77 and 78: LaserMUX TM Beam Combining Filters
Page 79 and 80: TECHNICAL NOTE Measurement of Optic
Page 81 and 82: VersaChrome ® Tunable Filters TECH
Page 83 and 84: VersaChrome ® Tunable Bandpass Fil
Page 85 and 86: VersaChrome Edge TM Tunable Filters
Page 87 and 88: Laser Wavelength Reference Table La
Page 89 and 90: Polarization Filters Common Specifi
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General Purpose Mirrors Semrock gen
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EdgeBasic TM Long / Short Wave Pass
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Edge Steepness and Transition Width
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RazorEdge ® Common Specifications
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Filter Types for Raman Spectroscopy
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MaxLine ® Laser-line Spectra and S
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Near Infrared Bandpass Filters Tran
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StopLine ® Single-notch Filter Com
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TECHNICAL NOTE Filter Spectra at No
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Working with Optical Density Optica
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LEARN MORE WITH OUR VIDEO COLLECTIO
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The Physics of Pixel Shift Pixel sh
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Get the Right Solution, Right Now W
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Semrock Master Catalog 2018

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