Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
BrightLine ® Laser Fluorescence<br />
Single-band and Longpass Filter Sets<br />
Super-resolution / TIRF filter sets guarantee 1 λ P-V RWE filter set performance<br />
Filter wavelengths precisely keyed to popular laser lines, with steep transitions from laser blocking to fluorescence transmission<br />
Minimal reflected wavefront distortion for even large diameter illumination beams<br />
Exceptionally high transmission to maximize system throughput, thus reducing acquisition time<br />
Deep blocking at laser wavelengths to minimize noise background<br />
Designed for the unique demands of TIRF laser excitation, the<br />
dichroic beamsplitter reflection range extends down to 350 nm<br />
allowing the combined use of photoactivation, UV sources with the<br />
normal excitation laser line. Users of uncaging and super-resolution<br />
techniques will appreciate this added functionality. Maximize SNR<br />
and minimize artifacts in TIRF, confocal, PALM, STORM, SIM, and<br />
other super-resolution techniques.<br />
Longpass sets allow for longer wavelengths to be detected and more light to be captured<br />
INDICATES SUPER-<br />
RESOLUTION / TIRF<br />
Cubes Laser<br />
Sets<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
NOTE: BrightLine Laser Fluorescence filter sets are optimized for laser excitation and inherently provide excellent image registration<br />
performance – when interchanging these sets with one another, minimal pixel shift is observed. Note that the laser filter sets are not<br />
designed to exhibit “zero pixel shift” performance when interchanging with BrightLine –ZERO TM<br />
filter sets. Images obtained with the<br />
laser filter sets exhibit excellent image registration not only with one another, but also with images obtained when no fluorescence<br />
filters are present (e.g. in DIC or other bright-field modes).<br />
Filter Set /<br />
Primary Fluorophores<br />
LF405/LP-C (Longpass)<br />
375 & 405 nm<br />
LF405-C<br />
375 & 405 nm<br />
LF442-C<br />
~ 440 & 441.6 nm<br />
LF488/LP-D (Longpass)<br />
473 & 488 nm<br />
LF488-D<br />
473 & 488 nm<br />
LF514-C<br />
514.5 & 515.0 nm<br />
LF561/LP-D<br />
559, 561.4, & 568.2 nm<br />
LF561-C<br />
559, 561.4, & 568.2 nm<br />
LF594/LP-D (Longpass)<br />
593.5,594, 594.1 nm<br />
LF594-D<br />
593.5, 594, 594.1 nm<br />
LF635/LP-C (Longpass)<br />
632.8, 635, & 647.1 nm<br />
LF635-C<br />
632.8, 635, & 647.1 nm<br />
Excitation<br />
(CWL/BW)<br />
Emission<br />
(CWL/BW)<br />
Dichroic<br />
(Laser) Filter Set Part Numbers Base Price<br />
390/40 405/LP 405 nm LF405/LP-C-000 $965<br />
390/40 452/45 405 nm LF405-C-000 $965<br />
448/20 482/25 442 nm LF442-C-000 $965<br />
482/18 488/LP 488 nm LF488/LP-D-000 $965<br />
482/18 525/45 488 nm LF488-D-000 $965<br />
510/10 542/27 514 nm LF514-C-000 $965<br />
561/14 561/LP 561 nm LF561/LP-D-000 $965<br />
561/14 609/54 561 nm LF561-C-000 $965<br />
591/6 594/LP 594 nm LF594/LP-D-000 $965<br />
591/6 647/57 594 nm LF594-D-000 $965<br />
640/14 635/LP 635 nm LF635/LP-C-000 $965<br />
640/14 676/29 635 nm LF635-C-000 $965<br />
Filter Specifications on page 34<br />
Tunable<br />
Filters<br />
See spectra graphs and ASCII data for these filter sets at<br />
www.semrock.com/SingleBand-Laser.aspx<br />
www.semrock.com/Longpass-Laser.aspx<br />
CUBES PAGE 35<br />
More<br />
38
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