Semrock Master Catalog 2018

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Fluorophores Single-band Sets BrightLine ® –ZERO TM Image Registration BrightLine –ZERO Fluorescence Filter Sets Only $99 ensures exact image registration when making multi-color composite images with BrightLine ® single-band sets. Not sure you need this? Keep in mind that BrightLine filters do not burn out, and the –ZERO option requires no calibration or special alignment, so why not cost-effectively future-proof your system? Join your many colleagues and demand the “–ZERO option” for certified image registration. To order, just add “–ZERO” to the end of the filter set part number. Allows you to create spatially registered multi-color composite images Hard coated for durability and reliability Ideal for demanding applications like: Co-localization fluorescence measurements Fluorescence In Situ Hybridization (FISH) Comparative Genomic Hybridization (CGH) Multiband Sets Property Value Comment Price Set-to-set Image Shift < ± 1 pixel Worst case image shift when interchanging BrightLine –ZERO filter sets, as measured relative to the mean image position for a large sample of filter sets. Analysis assumes collimated light in a standard microscope with a 200 mm focal length tube lens and 6.7 micron pixel size. Tested in popular microscope cubes. + $99 to the set price Cubes Laser Sets Available as a –ZERO Set BrightLine Single-band and Longpass Sets BrightLine LED Single-band Sets BrightLine LED Full Multiband & Pinkel Sets BrightLine FISH Sets FRET Sets Qdot Sets NOT Available as a –ZERO Set BrightLine Basic Sets BrightLine Laser Fluorescence Sets Brightline LED Sedat Multiband Sets BrightLine Multiband Sets Qdot Longpass Set Customer Selected Custom Sets TECHNICAL NOTE NLO Filters Individual Filters Dichroic Beamsplitters Tunable Filters What is Pixel Shift? Pixel shift results when a filter in an imaging path (the emitter and/or dichroic beamsplitter in a fluorescence microscope) with a non-zero wedge angle deviates the light rays to cause a shift of the image detected on a high-resolution CCD camera. When two or more images of the same object acquired using different filter sets are overlaid (in order to simultaneously view fluorescence from multiple fluorophores), any significant non-zero filter wedge angle means that the images will not be registered to identical pixels on the CCD camera. Composite images produced from conventional filter Hence, images produced by different fluorophores will not be accurately sets (above left), which typically have significant pixel shift, correlated or combined. are distorted, whereas BrightLine ZERO pixel shift filter sets (above right) yield precise multi-color images. Poor image registration, or pixel shift, results from the almost inevitable non-zero filter wedge angle. But low pixel shift is critical to obtain the best BrightLine ZERO imaging performance when exchanging filters during any measurements that involve BrightLine Hard Coating ZERO multiple exposures. <strong>Semrock</strong>’s advanced ion-beam-sputtering coating technology makes it possible for all BrightLine filters to be uniquely constructed from a single piece of glass, with the permanent hard coatings applied directly to the outside. This patented lower-loss and high-reliability construction inherently offers superior imaging performance. BrightLine –ZERO filter substrates are further manufactured and tested to the most exacting tolerances for certified “zero pixel shift” performance. With older soft-coated fluorescence filters, one is forced to use multiple substrates that are typically bonded together with adhesive, generally resulting in significant wedge angle and therefore pixel shift. To improve the imaging registration, extra processing steps, alignment steps, and/or compensating optics are required, resulting in added cost. By contrast, BrightLine –ZERO filters are inherently manufacturable and thus very affordable. Hard Coating Glass Hard Glass Coating Hard Coating Conventional Approach Conventional Uncoated Approach Uncoated Adhesive Adhesive Soft coating Soft Adhesive coating Adhesive Soft coating Soft coating Uncoated Uncoated More 36
BrightLine ® Laser Filter and Set Reference Tables Fluorescence Filter Sets Optimized for Lasers Laser Line 375 ± 3 nm 405 ± 5 nm ~ 440 nm 441.6 nm Popular Fluorophores Single-band Laser Sets Bandpass Page 38 Longpass Page 38 “Full Multiband”* Page 39 DAPI, BFP LF405-C LF405/LP-C LF405/488/594-A LF405/488/532/635-B LF405/488/561/635-B CFP LF442-C *Refer to page 30 for details on multiband filter set configurations Multiband Laser Sets “Pinkel”* Page 40 LF405/488/594-3X-B LF405/488/532/635-4X-B LF405/488/561/635-4X-B “Sedat”* Page 40 LF405/488/594-3X3M-B LF405/488/561/635- 4X4M-B Fluorophores Single-band Sets 473 ± 2 nm 488 +3/–2 nm 491 nm 514.5 nm 515 nm FITC, GFP LF488-D LF488/LP-D LF488/561-B LF405/488/594-A LF405/488/532/635-B LF405/488/561/635-B YFP LF514-C LF488/561-2X-C LF405/488/594-3X-B LF488/543/635-3X-A LF405/488/532/635-4X-B LF405/488/561/635-4X-B LF488/561-2X2M-C LF405/488/594-3X3M-B LF488/543/594-3X3M-A LF405/488/561/635- 4X4M-B Multiband Sets 532 nm TRITC LF405/488/532/635-B LF405/488/532/635-4X-B 543 nm TRITC, Cy3 LF488/543/635-3X-A LF488/543/594-3X3M-A 559 ± 5 nm 561.4 nm 568.2 nm RFPs (mCherry, LF561-C LF561/LP-D LF488/561-B Texas Red ® HcRed, DsRed), LF405/488/561/635-B LF488/561-2X-C LF405/488/561/635-4X-B LF488/561-2X2M-C LF405/488/561/635- 4X4M-B Cubes 593.5 nm 594 ± 0.3 nm 594.1 nm mCherry, mKate2, Alexa Fluor 594 , Texas Red ® LF594-D LF594/LP-D LF405/488/594-A LF405/488/594-3X-B LF405/488/594-3X3M-B LF488/543/594-3X3M-A 632.8 nm 635 +7/–0 nm 647.1 nm Cy5 , APC, Alexa 633 & 647 LF635-C LF635/LP-C LF405/488/532/635-B LF405/488/561/635-B LF488/543/635/-3X-A LF405/488/532/635-4X-B LF405/488/561/635-4X-B LF405/488/561/635- 4X4M-B Laser Sets Individual Fluorescence Filters Optimized for Lasers Laser Line Laser Description Single-Edge Laser Dichroics Page 71 − 73 Multiedge Laser Dichroic Page 71 & 73 Laser Combiners/ Separators Page 75 Yokogawa CSU Filters Page 76 Laser Longpass Filters Page 90 ~ 375 GaN diode • • • ~ 405 GaN diode • • • • • ~ 440 Diode • • • 441.6 HeNe gas • • • • 457.9 Ar-ion gas • • • • ~ 470 Diode • • • 473.0 Doubled DPSS • • • • • 488.0 Ar-ion gas • • • • • ~ 488 Doubled OPS • • • • • 491.0 Doubled DPSS • • • • 505.0 • • 514.5 Ar-ion gas • • • • 515.0 Doubled DPSS • • • • 532.0 Doubled DPSS • • • • • 543.5 HeNe gas • • ~559 • 561.4 Doubled DPSS • • • • • 568.2 Kr-ion gas • • • • • 593.5 Doubled DPSS • • • • 594.1 HeNe gas • • • • 632.8 HeNe gas • • • • • ~ 635 Diode • • • • • 647.1 Kr-ion gas • • • • NLO Filters Individual Filters Dichroic Beamsplitters Tunable Filters Multiphoton laser fluorescence filters are available for blocking ranges of 680-1600 nm, see page 44 37 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37: Fluorescence Filter Cubes See onlin
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
Page 77 and 78: LaserMUX TM Beam Combining Filters
Page 79 and 80: TECHNICAL NOTE Measurement of Optic
Page 81 and 82: VersaChrome ® Tunable Filters TECH
Page 83 and 84: VersaChrome ® Tunable Bandpass Fil
Page 85 and 86: VersaChrome Edge TM Tunable Filters
Page 87 and 88: Laser Wavelength Reference Table La
Page 89 and 90:
Polarization Filters Common Specifi
Page 91 and 92:
General Purpose Mirrors Semrock gen
Page 93 and 94:
EdgeBasic TM Long / Short Wave Pass
Page 95 and 96:
Edge Steepness and Transition Width
Page 97 and 98:
RazorEdge ® Common Specifications
Page 99 and 100:
Filter Types for Raman Spectroscopy
Page 101 and 102:
MaxLine ® Laser-line Spectra and S
Page 103 and 104:
Near Infrared Bandpass Filters Tran
Page 105 and 106:
StopLine ® Single-notch Filter Com
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TECHNICAL NOTE Filter Spectra at No
Page 109 and 110:
Working with Optical Density Optica
Page 111 and 112:
LEARN MORE WITH OUR VIDEO COLLECTIO
Page 113 and 114:
The Physics of Pixel Shift Pixel sh
Page 115 and 116:
Get the Right Solution, Right Now W
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Semrock Master Catalog 2018

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