Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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Fluorophores<br />
Single-band<br />
Sets<br />
BrightLine ® –ZERO TM Image Registration<br />
BrightLine –ZERO Fluorescence Filter Sets<br />
Only $99 ensures exact image registration when making multi-color composite images with BrightLine ®<br />
single-band sets. Not sure you<br />
need this? Keep in mind that BrightLine filters do not burn out, and the –ZERO option requires no calibration or special alignment,<br />
so why not cost-effectively future-proof your system? Join your many colleagues and demand the “–ZERO option” for certified image<br />
registration. To order, just add “–ZERO” to the end of the filter set part number.<br />
Allows you to create spatially registered multi-color composite images<br />
Hard coated for durability and reliability<br />
Ideal for demanding applications like:<br />
Co-localization fluorescence measurements<br />
Fluorescence In Situ Hybridization (FISH)<br />
Comparative Genomic Hybridization (CGH)<br />
Multiband<br />
Sets<br />
Property Value Comment Price<br />
Set-to-set Image Shift < ± 1 pixel Worst case image shift when interchanging BrightLine –ZERO filter sets, as measured<br />
relative to the mean image position for a large sample of filter sets. Analysis assumes<br />
collimated light in a standard microscope with a 200 mm focal length tube lens and<br />
6.7 micron pixel size. Tested in popular microscope cubes.<br />
+ $99 to the<br />
set price<br />
Cubes Laser<br />
Sets<br />
Available as a –ZERO Set<br />
BrightLine Single-band and Longpass Sets<br />
BrightLine LED Single-band Sets<br />
BrightLine LED Full Multiband & Pinkel Sets<br />
BrightLine FISH Sets<br />
FRET Sets<br />
Qdot Sets<br />
NOT Available as a –ZERO Set<br />
BrightLine Basic Sets<br />
BrightLine Laser Fluorescence Sets<br />
Brightline LED Sedat Multiband Sets<br />
BrightLine Multiband Sets<br />
Qdot Longpass Set<br />
Customer Selected Custom Sets<br />
TECHNICAL NOTE<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
What is Pixel Shift?<br />
Pixel shift results when a filter in an imaging path (the emitter and/or dichroic<br />
beamsplitter in a fluorescence microscope) with a non-zero wedge angle<br />
deviates the light rays to cause a shift of the image detected on a high-resolution<br />
CCD camera. When two or more images of the same object acquired using<br />
different filter sets are overlaid (in order to simultaneously view fluorescence<br />
from multiple fluorophores), any significant non-zero filter wedge angle means<br />
that the images will not be registered to identical pixels on the CCD camera. Composite images produced from conventional filter<br />
Hence, images produced by different fluorophores will not be accurately<br />
sets (above left), which typically have significant pixel shift,<br />
correlated or combined.<br />
are distorted, whereas BrightLine ZERO pixel shift filter sets<br />
(above right) yield precise multi-color images.<br />
Poor image registration, or pixel shift, results from the almost inevitable<br />
non-zero filter wedge angle. But low pixel shift is critical to obtain the best<br />
BrightLine ZERO<br />
imaging performance when exchanging filters during any measurements that involve<br />
BrightLine Hard Coating ZERO<br />
multiple exposures.<br />
<strong>Semrock</strong>’s advanced ion-beam-sputtering coating technology makes it possible for all<br />
BrightLine filters to be uniquely constructed from a single piece of glass, with the permanent<br />
hard coatings applied directly to the outside. This patented lower-loss and high-reliability<br />
construction inherently offers superior imaging performance. BrightLine –ZERO filter substrates<br />
are further manufactured and tested to the most exacting tolerances for certified “zero pixel<br />
shift” performance.<br />
With older soft-coated fluorescence filters, one is forced to use multiple substrates that are<br />
typically bonded together with adhesive, generally resulting in significant wedge angle and<br />
therefore pixel shift. To improve the imaging registration, extra processing steps, alignment<br />
steps, and/or compensating optics are required, resulting in added cost. By contrast,<br />
BrightLine –ZERO filters are inherently manufacturable and thus very affordable.<br />
Hard Coating<br />
Glass<br />
Hard Glass Coating<br />
Hard Coating<br />
Conventional Approach<br />
Conventional<br />
Uncoated<br />
Approach<br />
Uncoated<br />
Adhesive<br />
Adhesive Soft coating<br />
Soft Adhesive coating<br />
Adhesive Soft coating<br />
Soft coating<br />
Uncoated<br />
Uncoated<br />
More<br />
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