Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets Cubes Laser Sets NLO Filters Individual Filters Dichroic Beamsplitters BrightLine ® Filter Common Specifications (for filters in sets pages 15–33) Exciter and Emitter Specifications (except where otherwise noted) Property Specification Comment Guaranteed Transmission > 93% Typical Transmission > 97% Except BrightLine Basic and Qdot ® : > 90%; except multiband Averaged over the passband Except BrightLine Basic and Qdot: > 94% Averaged over the passband Angle of Incidence 0° ± 5° Range of angles over which optical specs are guaranteed for collimated light Cone Half Angle 7° Autofluorescence Low Filter performance is likely to remain satisfactory up to 10° centered around the nominal Angle of Incidence Transverse Dimensions 25.0 mm Except Leica sizes, see www.semrock.com/microscope-compatibility.aspx Transverse Tolerance + 0.0 / - 0.1 mm Exciter Thickness 5.0 mm Black-anodized aluminium ring Emitter Thickness 3.5 mm Black-anodized aluminium ring Thickness Tolerance ± 0.1 mm Black-anodized aluminium ring Exciter Clear Aperture > 21 mm Except Leica filters: > 85% Emitter Clear Aperture > 22 mm Except BrightLine Basic & Qdot: > 21 mm; except Leica filters: > 85% Scratch-Dig 60-40 Ring Housing Material Blocking Aluminum, black anodized Except BrightLine Basic: 80-50 Measured within clear aperture BrightLine filters have blocking far exceeding OD 6 (except BrightLine Basic: OD 5) as needed to ensure the blackest background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our proprietary filter design software. Orientation Arrow on ring indicates preferred direction of propagation of light (see page 27) Dichroic Beamsplitter Specifications (except where otherwise noted) Property Specification Comment Guaranteed Transmission > 93% Averaged over the specified band; except multiband and BrightLine Basic Typical Transmission > 97% Averaged over the specified band; except BrightLine Basic Reflection > 98% Except BrightLine Basic: > 90%; and multiband Averaged over the specified band Angle of Incidence 45° ± 1.5° Range of angles over which optical specs are guaranteed for collimated light Cone Half Angle 2° Filter performance is likely to remain satisfactory up to 3° Centered around the nominal Angle of Incidence Autofluorescence Ultra-low Transverse Dimensions 25.2 x 35.6 mm Except Leica sizes, see www.semrock.com Transverse Tolerance ± 0.1 mm Thickness 1.05 mm Except where otherwise noted Thickness Tolerance ± 0.05 mm Clear Aperture > 80% Elliptical Surface Quality 60-40 Except BrightLine Basic: 80-50 Measured within clear aperture Edge Chipping Per ANSI/OEOSC OP1.002-2006, American Standard Orientation Reflective coating side should face toward light source and sample (see page 27) General Filter Specifications (all BrightLine filters) For Laser Dichroic Specifications, see page 72 Tunable Filters Property Coating Type Reliability and Durability Microscope Compatibility Specification Sputtered Hard-coated technology with epoxy-free, single-substrate construction for unrivaled filter life span and no “burn-out” even when subjected to high optical intensities for a prolonged period of time. BrightLine filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards. All BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss and Aperio microscopes. See spectra graphs and ASCII data for these filter sets at www.semrock.com More 34
Fluorescence Filter Cubes See online filter installation video Microscope Brand / Compatible Microscopes Aperio <strong>Semrock</strong> Cube Designation Cube Price* Cube Part Number Filter Set Part Number Mounted in Cube ScanScope FL AMF $465 AMF -AMF Nikon Fluorophores Single-band Sets TE2000, 80i, 90i, 50i, 55i, Eclipse Ti, Ni, Ci, and any using the Epi-fluor Illuminator E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series TE2000 $395 NTE -NTE Quadfluor $395 NQF -NQF Multiband Sets Olympus AX70, BX, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81 Compatible with the BX53, BX63 upright microscopes and also standard beam diameter applications for the IX53, IX73, and IX83 inverted microscopes. Fluorescence Filter holder for Olympus IX3 Microscopes For large beam diameter and lower tier applications in the IX73 and IX83 model microscopes. Requires a 32mm emitter and exciter and a 32 x 44 mm dichroic beamsplitter. Zeiss Axio Imager, Axiostar Plus, Axioskop 40, Axio Observer, Axioplan2i, Axioplan2ie, Axiovert200, and Axioskop2 (post-2001), Axiovert 40, Axio Examiner, and Axio Scope A1 U-MF2 $435 OMF -OMF U-FF $435 OFF -OFF IX3-FFXL $595 OFX -OFX FL CUBE EC P&C $275 ZHE -ZHE Leica - BrightLine Basic, TRP-A, QDLP-B, and Laser Fluorescence sets are not sold as –ZERO compatible sets. DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI-4000 B, DM-5000, DM-5500, DM-6000 and DMI6000 B DM-K $350 LDMK** -LDMK- ZERO Cubes Laser Sets NLO Filters DMi8 DMi8 P-cube $435 LDMP -LDMP- ZERO * Cube price when purchased separately or with a set. To have your set mounted at no charge, replace “-000” in the set part number with the cube part number from above (e.g. use FITC-3540C-NTE). ** Non-standard sets: Filter sets mounted in cubes that require non-standard filter shapes and sizes may not be available for same-day shipping. Multi-exciter sets are also available with 32 mm diameter exciters. See website for current pricing. NOTE If you use a Leica microscope, all BrightLine single-band bandpass filters in “Pinkel” and “Sedat” sets come with standard 25 mm (32 mm optional) exciters and 25 mm emitters, which are packaged separately for convenient mounting in standard filter wheels. For set part numbers for Leica microscopes, see www.semrock.com. Individual Filters Dichroic Beamsplitters Tunable Filters 35 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35: BrightLine ® Multiband Fluorescenc
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
Page 77 and 78: LaserMUX TM Beam Combining Filters
Page 79 and 80: TECHNICAL NOTE Measurement of Optic
Page 81 and 82: VersaChrome ® Tunable Filters TECH
Page 83 and 84: VersaChrome ® Tunable Bandpass Fil
Page 85 and 86: VersaChrome Edge TM Tunable Filters
Page 87 and 88:
Laser Wavelength Reference Table La
Page 89 and 90:
Polarization Filters Common Specifi
Page 91 and 92:
General Purpose Mirrors Semrock gen
Page 93 and 94:
EdgeBasic TM Long / Short Wave Pass
Page 95 and 96:
Edge Steepness and Transition Width
Page 97 and 98:
RazorEdge ® Common Specifications
Page 99 and 100:
Filter Types for Raman Spectroscopy
Page 101 and 102:
MaxLine ® Laser-line Spectra and S
Page 103 and 104:
Near Infrared Bandpass Filters Tran
Page 105 and 106:
StopLine ® Single-notch Filter Com
Page 107 and 108:
TECHNICAL NOTE Filter Spectra at No
Page 109 and 110:
Working with Optical Density Optica
Page 111 and 112:
LEARN MORE WITH OUR VIDEO COLLECTIO
Page 113 and 114:
The Physics of Pixel Shift Pixel sh
Page 115 and 116:
Get the Right Solution, Right Now W
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