Semrock Master Catalog 2018

Fluorophores
Single-band
Sets
Multiband
Sets
Cubes Laser
Sets
NLO
Filters
Individual
Filters
Dichroic
Beamsplitters
BrightLine ® Filter Common Specifications (for filters in sets pages 15–33)
Exciter and Emitter Specifications (except where otherwise noted)
Property Specification Comment
Guaranteed Transmission > 93%
Typical Transmission > 97%
Except BrightLine Basic and Qdot ® : > 90%; except multiband
Averaged over the passband
Except BrightLine Basic and Qdot: > 94%
Averaged over the passband
Angle of Incidence 0° ± 5° Range of angles over which optical specs are guaranteed for collimated light
Cone Half Angle 7°
Autofluorescence
Low
Filter performance is likely to remain satisfactory up to 10°
centered around the nominal Angle of Incidence
Transverse Dimensions 25.0 mm Except Leica sizes, see www.semrock.com/microscope-compatibility.aspx
Transverse Tolerance
+ 0.0 / - 0.1 mm
Exciter Thickness 5.0 mm Black-anodized aluminium ring
Emitter Thickness 3.5 mm Black-anodized aluminium ring
Thickness Tolerance ± 0.1 mm Black-anodized aluminium ring
Exciter Clear Aperture > 21 mm Except Leica filters: > 85%
Emitter Clear Aperture > 22 mm Except BrightLine Basic & Qdot: > 21 mm; except Leica filters: > 85%
Scratch-Dig 60-40
Ring Housing Material
Blocking
Aluminum, black anodized
Except BrightLine Basic: 80-50
Measured within clear aperture
BrightLine filters have blocking far exceeding OD 6 (except BrightLine Basic: OD 5) as needed to ensure the
blackest background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy
applications using our proprietary filter design software.
Orientation Arrow on ring indicates preferred direction of propagation of light (see page 27)
Dichroic Beamsplitter Specifications (except where otherwise noted)
Property Specification Comment
Guaranteed Transmission > 93% Averaged over the specified band; except multiband and BrightLine Basic
Typical Transmission > 97% Averaged over the specified band; except BrightLine Basic
Reflection > 98%
Except BrightLine Basic: > 90%; and multiband
Averaged over the specified band
Angle of Incidence 45° ± 1.5° Range of angles over which optical specs are guaranteed for collimated light
Cone Half Angle 2°
Filter performance is likely to remain satisfactory up to 3°
Centered around the nominal Angle of Incidence
Autofluorescence
Ultra-low
Transverse Dimensions 25.2 x 35.6 mm Except Leica sizes, see www.semrock.com
Transverse Tolerance
± 0.1 mm
Thickness 1.05 mm Except where otherwise noted
Thickness Tolerance
± 0.05 mm
Clear Aperture > 80% Elliptical
Surface Quality 60-40
Except BrightLine Basic: 80-50
Measured within clear aperture
Edge Chipping
Per ANSI/OEOSC OP1.002-2006, American Standard
Orientation Reflective coating side should face toward light source and sample (see page 27)
General Filter Specifications (all BrightLine filters)
For Laser Dichroic Specifications, see page 72
Tunable
Filters
Property
Coating Type
Reliability and Durability
Microscope Compatibility
Specification
Sputtered
Hard-coated technology with epoxy-free, single-substrate construction for unrivaled filter life span and no
“burn-out” even when subjected to high optical intensities for a prolonged period of time. BrightLine filters
are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards.
All BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss and Aperio microscopes.
See spectra graphs and ASCII data for these filter sets at www.semrock.com
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