Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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BrightLine ® Multiband Fluorescence Sets<br />
Full Multiband Filter Sets – multiband exciter, emitter and beamsplitter<br />
Filter Set /<br />
Primary Fluorophores<br />
Multiband<br />
Excitation<br />
(CWL/BW)<br />
Multiband<br />
Emission<br />
(CWL/BW)<br />
Multiedge<br />
Dichroic<br />
(Edge)<br />
Filter Set Part<br />
Numbers<br />
Base<br />
Price<br />
Fluorophores<br />
DA/FI-A (Dual-Band Full Multiband) DA/FI-A-000 $1025<br />
Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350<br />
Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488<br />
387/11<br />
480/29<br />
433/38<br />
530/40<br />
403 nm<br />
502 nm<br />
CFP/YFP-A (Dual-Band Full Multiband) CFP/YFP-A-000 $1025<br />
Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1<br />
Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123<br />
416/25<br />
501/18<br />
464/23<br />
547/31<br />
440 nm<br />
520 nm<br />
GFP/DsRed-A (Dual-Band Full Multiband) GFP/DsRed-A-000 $1025<br />
Green: GFP, rsGFP, FITC, Alexa Fluor ® 488<br />
Red: DsRed, TRITC, Cy3 , Texas Red ® , Alexa Fluor ® 568 & 594<br />
468/34<br />
553/26<br />
512/23<br />
630/91<br />
493 nm<br />
574 nm<br />
FITC/TxRed-A (Dual-Band Full Multiband) FITC/TxRed-A-000 $1025<br />
Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488<br />
Red: Texas Red ® , mCherry, Alexa Fluor ® 568 & 594<br />
479/38<br />
585/27<br />
524/29<br />
628/33<br />
505 nm<br />
606 nm<br />
Cy3/Cy5-A (Dual-Band Full Multiband) Cy3/Cy5-A-000 $1025<br />
Yellow: Cy3 , DsRed, Alexa Fluor ® 555<br />
Red: Cy5 , SpectrumFRed , Alexa Fluor ® 647 & 660<br />
534/36<br />
635/31<br />
577/24<br />
690/50<br />
560 nm<br />
659 nm<br />
DA/FI/TR-A (Triple-Band Full Multiband) DA/FI/TR-A-000 $1225<br />
Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350<br />
Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488<br />
Orange: TRITC, DsRed, Cy3 , Alexa Fluor ® 555<br />
387/11<br />
478/24<br />
555/19<br />
433/36<br />
517/23<br />
613/61<br />
403 nm<br />
497 nm<br />
574 nm<br />
DA/FI/TX-B (Triple-Band Full Multiband) DA/FI/TX-B-000 $1225<br />
Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350<br />
Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488<br />
Red: Texas Red ® , MitoTracker Red, Alexa Fluor ® 568 & 594<br />
407/14<br />
494/20<br />
576/20<br />
457/22<br />
530/20<br />
628/28<br />
436 nm<br />
514 nm<br />
604 nm<br />
DA/FI/TR/Cy5-A (Quad-Band Full Multiband) DA/FI/TR/Cy5-A-000 $1375<br />
Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350<br />
Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488<br />
Orange: TRITC, DsRed, Cy3 , Alexa Fluor ® 555<br />
Red: Texas Red ® , MitoTracker Red, Alexa Fluor ® 568 & 594<br />
387/11<br />
485/20<br />
559/25<br />
649/13<br />
440/40<br />
521/21<br />
607/34<br />
700/45<br />
410 nm<br />
504 nm<br />
582 nm<br />
669 nm<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Cubes<br />
Laser<br />
Sets<br />
See spectra graphs and ASCII data for these filter sets at<br />
www.semrock.com/FullMultiBand-Broadband.aspx<br />
Filter Specifications on page 34<br />
CUBES PAGE 35<br />
NLO<br />
Filters<br />
PRODUCT NOTE<br />
<strong>Semrock</strong> manufactures multiband fluorescence filters with passband, edge<br />
steepness, and blocking performance that rival the best single-band filters,<br />
and all with the superior, “no burn-out” durability of hard coatings. In fact,<br />
every filter in every BrightLine filter set, including these multiband sets, is<br />
made with the same, durable hard-coating technology.<br />
<strong>Semrock</strong> always provides:<br />
The highest transmission, blocking and edge steepness for<br />
dazzling visual and digital imaging<br />
Hard, dielectric coatings for every filter, including UV exciters<br />
for ‘no burn-out’ performance<br />
Spectrally complex filters are a specialty. The world’s only five<br />
color multiband set and a large selection of quad, triple, and<br />
dual band sets available and also guaranteed in stock.<br />
100<br />
90<br />
Measured<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
350 480 610 740 870 1000<br />
Wavelength (nm)<br />
Transmission (%)<br />
Graph above shows typical measured transmission<br />
of the FF01-432/515/595/681/809 filter<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
31<br />
More