Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets Cubes Laser Sets BrightLine ® Multiband Fluorescence Sets TECHNICAL NOTE Multiband Filter Set Configurations The ability to label multiple, distinct objects of interest in a single sample greatly enhances the power of fluorescence imaging. One way to achieve high-quality images of such samples has been to take multiple photographs while switching single-band filter cubes between photographs, and then later to combine these photographs electronically. Limitations to this approach historically included “pixel shift” among the multiple monochrome images, and the speed with which a complete multicolor image could be captured. Semrock solved the problem of “pixel shift” with its BrightLine ZERO technology, and the single-band filter cube approach remains the best technique for achieving images with the highest contrast and lowest bleedthrough possible. But with the increasing demand for high-speed imaging, especially for live-cell real-time analysis using fluorescent protein labels, there is a need for an alternative to the single-band filter cube approach that does not sacrifice too much image fidelity. Semrock’s advanced multiband optical filter technology brings simultaneous multicolor imaging to a new level. There are three types of multiband filter sets for simultaneous multicolor imaging. The “full multiband” configuration uses all multiband filters – exciter, emitter, and dichroic beamsplitter – and is ideal for “Full Multiband” Configuration (Multiband exciter, multiband emitter, & multiband dichroic) direct visualization, such as locating areas of interest on a sample. This approach is quick and easy to implement, and is compatible with all standard fluorescence microscopes. However, it requires a color camera for electronic imaging and cannot eliminate fluorophore bleedthrough. The “Pinkel” configuration uses single-band exciters in a filter wheel with multiband emitter and dichroic filters. It offers an economical way to achieve very high-speed, high-contrast, simultaneous multi-color imaging. This approach is based on a monochrome CCD camera, which is less expensive and offers better resolution and noise performance than color cameras. While bleedthrough is reduced relative to the fullmultiband approach, some bleedthrough is still possible since all emission bands are imaged simultaneously. The “Sedat” configuration uses single-band exciters and singleband emitters in synchronized filter wheels, with a multiband dichroic beamsplitter. This approach provides the best image fidelity for high-speed simultaneous multi-color imaging, though it requires a larger investment in system hardware. In fact, Semrock is your only source of “full multiband” quadband filter sets and the unique penta “Pinkel” and “Sedat” set. “Pinkel” Configuration (Multiband emitter, multiband dichroic, & single-band exciters) “Sedat” Configuration (Multiband dichroic, single-band exciters, & single-band emitters) NLO Filters Individual Filters Dichroic Beamsplitters “Full Multiband” Image Multi-color image captured with a color CCD camera “Pinkel” and “Sedat” Composite Image Single-color images are combined electronically to produce one high-fidelity, multi-color image Tunable Filters T-Cell and Antigen Presenting Cell (APC) conjugates demonstrating an immunologic synapse. Samples courtesy of Beth Graf and Dr. Jim Miller at the University of Rochester Medical Center More 30
BrightLine ® Multiband Fluorescence Sets Full Multiband Filter Sets – multiband exciter, emitter and beamsplitter Filter Set / Primary Fluorophores Multiband Excitation (CWL/BW) Multiband Emission (CWL/BW) Multiedge Dichroic (Edge) Filter Set Part Numbers Base Price Fluorophores DA/FI-A (Dual-Band Full Multiband) DA/FI-A-000 $1025 Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488 387/11 480/29 433/38 530/40 403 nm 502 nm CFP/YFP-A (Dual-Band Full Multiband) CFP/YFP-A-000 $1025 Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123 416/25 501/18 464/23 547/31 440 nm 520 nm GFP/DsRed-A (Dual-Band Full Multiband) GFP/DsRed-A-000 $1025 Green: GFP, rsGFP, FITC, Alexa Fluor ® 488 Red: DsRed, TRITC, Cy3 , Texas Red ® , Alexa Fluor ® 568 & 594 468/34 553/26 512/23 630/91 493 nm 574 nm FITC/TxRed-A (Dual-Band Full Multiband) FITC/TxRed-A-000 $1025 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488 Red: Texas Red ® , mCherry, Alexa Fluor ® 568 & 594 479/38 585/27 524/29 628/33 505 nm 606 nm Cy3/Cy5-A (Dual-Band Full Multiband) Cy3/Cy5-A-000 $1025 Yellow: Cy3 , DsRed, Alexa Fluor ® 555 Red: Cy5 , SpectrumFRed , Alexa Fluor ® 647 & 660 534/36 635/31 577/24 690/50 560 nm 659 nm DA/FI/TR-A (Triple-Band Full Multiband) DA/FI/TR-A-000 $1225 Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488 Orange: TRITC, DsRed, Cy3 , Alexa Fluor ® 555 387/11 478/24 555/19 433/36 517/23 613/61 403 nm 497 nm 574 nm DA/FI/TX-B (Triple-Band Full Multiband) DA/FI/TX-B-000 $1225 Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488 Red: Texas Red ® , MitoTracker Red, Alexa Fluor ® 568 & 594 407/14 494/20 576/20 457/22 530/20 628/28 436 nm 514 nm 604 nm DA/FI/TR/Cy5-A (Quad-Band Full Multiband) DA/FI/TR/Cy5-A-000 $1375 Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor ® 350 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor ® 488 Orange: TRITC, DsRed, Cy3 , Alexa Fluor ® 555 Red: Texas Red ® , MitoTracker Red, Alexa Fluor ® 568 & 594 387/11 485/20 559/25 649/13 440/40 521/21 607/34 700/45 410 nm 504 nm 582 nm 669 nm Single-band Sets Multiband Sets Cubes Laser Sets See spectra graphs and ASCII data for these filter sets at www.semrock.com/FullMultiBand-Broadband.aspx Filter Specifications on page 34 CUBES PAGE 35 NLO Filters PRODUCT NOTE Semrock manufactures multiband fluorescence filters with passband, edge steepness, and blocking performance that rival the best single-band filters, and all with the superior, “no burn-out” durability of hard coatings. In fact, every filter in every BrightLine filter set, including these multiband sets, is made with the same, durable hard-coating technology. Semrock always provides: The highest transmission, blocking and edge steepness for dazzling visual and digital imaging Hard, dielectric coatings for every filter, including UV exciters for ‘no burn-out’ performance Spectrally complex filters are a specialty. The world’s only five color multiband set and a large selection of quad, triple, and dual band sets available and also guaranteed in stock. 100 90 Measured 80 70 60 50 40 30 20 10 0 350 480 610 740 870 1000 Wavelength (nm) Transmission (%) Graph above shows typical measured transmission of the FF01-432/515/595/681/809 filter Individual Filters Dichroic Beamsplitters Tunable Filters 31 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31: BrightLine ® Multiband LED Filter
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
Page 77 and 78: LaserMUX TM Beam Combining Filters
Page 79 and 80: TECHNICAL NOTE Measurement of Optic
Page 81 and 82: VersaChrome ® Tunable Filters TECH
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VersaChrome ® Tunable Bandpass Fil
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VersaChrome Edge TM Tunable Filters
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Laser Wavelength Reference Table La
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Polarization Filters Common Specifi
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General Purpose Mirrors Semrock gen
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EdgeBasic TM Long / Short Wave Pass
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Edge Steepness and Transition Width
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RazorEdge ® Common Specifications
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Filter Types for Raman Spectroscopy
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MaxLine ® Laser-line Spectra and S
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Near Infrared Bandpass Filters Tran
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StopLine ® Single-notch Filter Com
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TECHNICAL NOTE Filter Spectra at No
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Working with Optical Density Optica
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LEARN MORE WITH OUR VIDEO COLLECTIO
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The Physics of Pixel Shift Pixel sh
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Get the Right Solution, Right Now W
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