Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets Cubes Laser Sets NLO Filters TECHNICAL NOTE BrightLine ® LED-Optimized Fluorescence Filter Sets Over the past decade, LED-based light engines have largely replaced gas-discharge lamps (or arc lamps) for fluorescence imaging because LEDs enable faster channel switching, deliver discrete output wavelengths, consume less energy, and offer significantly longer lifetimes. An important factor to consider in this change in excitation technology is that the spectral signature and energy distribution of LED light engines differ from those of arc lamps. This is illustrated in Figure 1. Transmission (%) 100 90 80 70 60 50 40 30 20 LED Broadband (Lumencor SOLA light engine) Lamp Xenon Arc (Xenon Corp) 10 0 350 450 550 650 750 Wavelength (nm) Figure 1: “Comparison of the spectra of a Xenon arc lamp with a Broadband LED light engine” BrightLine LED-based Light Engine Filter Set Solutions Because traditional filter sets are tuned to match the output spectra of arc lamps, the mismatch between the illumination spectra of the new LED lamps with the transmission spectra of the traditional filter sets causes a reduction in the brightness of the illumination reaching the fluorophores. As an example, consider the case of the DA/FI/TR/Cy5-4X-B (traditional) quadband filter set. The excitation filters in this set are optimized for the output spectrum of Xenon (Xe) or Mercury (Hg) arc lamps. When the light source is changed to an LED light engine of similar brightness, the overall transmitted energy through the four excitation channels is reduced, and within the Cy5 band, the excitation intensity is extremely low. To address the loss of transmitted energy caused by spectrum mismatch, Semrock developed a new generation of filter sets that match the excitation filters to the unique spectral peaks of the most popular LED-based light engines on the market today. Take, for example, the Cy5 channel of the previously mentioned quad-band set. Figure 2 shows the improvement of transmitted energy when, for an LED light engine, the selected excitation filter is changed from the FF01-650/13 (traditional Cy5 exciter) to the corresponding FF01-635/18 (LED Cy5 exciter). It can be seen that the peak for the FF01-635/18 100 has been centered over the LED peak. 90 Designing new excitation filters for the LED-optimized sets has resulted in improvement of the transmitted signal for the DAPI and TRITC bands by 35% each in comparison to the traditional sets. Most importantly, the signal within the Cy5 band is restored, with an improvement of 750%. Transmission (%) 80 70 60 50 40 LED Broadband (Lumencor SOLA light engine) FF01-650/13 FF01-635/18 30 Individual Filters Dichroic Beamsplitters The Semrock BrightLine Benefit The new generation of LED-optimized BrightLine filter sets offers several benefits over traditional multiband filter sets: Excellent Signal Quality: Excitation filters are spectrally positioned to deliver the brightest signal and the best signal-to-noise ratio in the industry. 20 10 0 350 450 550 650 Wavelength (nm) Figure 2: “SearchLight simulation comparing the transmission through a FF01-650/13 (Cy5 exciter of traditional multiband filter set) and a FF01- 635/18 (Cy5 exciter of the multiband set optimized for LED Light Engines) when illuminated by a typical LED light engine.” Reduced Complexity and Cost: Common excitation filters can be used for both single-band and corresponding multiband configurations. This interchangeability provides a seamless transition between single-band and multiband imaging when configuring the excitation filters into the LED-based light engine and can also provide significant cost savings. Wide Selection: The BrightLine LED filter set family includes over twenty filter sets, supporting single-band, full multiband, Pinkel, and Sedat configurations. The filter sets include penta-band (5 channel), quad-band (4-channel), and triple-band (3 channel) configurations for the most popular fluorophores. 750 Tunable Filters Affordable Eight-Color Imaging: The penta-band LED-DA/FI/TR/Cy5/Cy7-5X-A and triple-band LED-CFP/YFP/mCherry-3X-A filter sets have complementary channels that provide an easy off-the-shelf solution to image eight fluorophores within a sample. The separation among all eight channels of the penta- and triple-band filters makes eight-color imaging easy and affordable (Figure 3). (continued) More 26
(continued from previous page) Fluorophores Transmission (%) 100 90 80 70 60 50 40 LED-DA/FI/TR/Cy5/Cy7-5X-A FF01-378/52 FF01-474/27 FF01-554/23 FF01-635/18 FF01-735/28 FF01-432/515/595/681/809 FF409/493/573/652/759-Di01 Figure 3: “SearchLight simulation demonstrating the compatibility of the LED-DA/FI/TR/Cy5/Cy7-5XA (penta-band LED set) and the LED-CFP/YFP/mCherry- 3X-A (triple-band LED set)” Single-band Sets 30 20 10 0 350 450 550 650 750 850 Wavelength (nm) Multiband Sets Transmission (%) 100 90 80 70 60 50 40 LED-CFP/YFP/mCherry-3X-A FF02-438/24 FF01-509/22 FF01-578/21 FF01-475/543/702 FF459/526/596-Di01 Cubes 30 20 10 0 350 450 550 650 750 850 Wavelength (nm) Laser Sets PRODUCT NOTE NLO Filters Orientation of Filters Because of the durability of Semrock filters, you can easily populate filter cubes, filter sliders, and filter wheels yourself without fear of damaging the filters. To maximize intended transmission and blocking and to minimize autofluorescence, filters must always be oriented so that light is incident on a specific surface of the filter. This note describes the correct orientation for the different filter types. Orienting Housed Excitation and Emission Filters Semrock exciter and emitter filters mounted in housings feature an alignment arrow on the housing; see the illustrations below. Orient such a filter so that the arrow points in the direction of light propagation. For microscopes, the exciter filter arrow should point away from the light source and toward the dichroic beamsplitter, and the emitter filter arrow should point away from the dichroic beamsplitter and toward the eye, detector, or camera. Dichroic beamsplitters are rarely mounted in housings. See below for guidance. light source emitter dichroic exciter BrightLine ® sample BrightLine ® to eye or camera reflective coating side Orienting Dichroic Beamsplitters and Other Unhoused Optical Filters Dichroic beamsplitters and other unhoused optical filters feature orientation marks that identify the coated surface upon which light must be incident. An orientation mark is placed either on the front surface of the filter, or on the edge of the filter as a caret (^) mark. The different types of orientation marks are shown in the following drawings along with the corresponding orientation guidance. Semrock logo: The logo is on the surface facing the incident light. Line: A short line is on the surface facing the incident light. The line may be easier to see if viewed at an oblique angle. Dot: A small dot is on the surface facing the incident light. The dot may be easier to see if viewed at an oblique angle. Caret: A caret on the edge of the filter points in the direction of light travel. When the viewer faces the surface that receives the incident light, the caret points away from the viewer. CAUTION: A number of dichroic beamsplitters have coatings on both surfaces. Always use the above instructions to identify the coated surface that should face the incident light! If you encounter any ambiguity or difficulty, please contact Semrock for assistance in identifying the surface orientation. Individual Filters Dichroic Beamsplitters Tunable Filters 27 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27: BrightLine ® Basic TM Best-value S
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
Page 77 and 78: LaserMUX TM Beam Combining Filters
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TECHNICAL NOTE Measurement of Optic
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VersaChrome ® Tunable Filters TECH
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VersaChrome ® Tunable Bandpass Fil
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VersaChrome Edge TM Tunable Filters
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Laser Wavelength Reference Table La
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Polarization Filters Common Specifi
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General Purpose Mirrors Semrock gen
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EdgeBasic TM Long / Short Wave Pass
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Edge Steepness and Transition Width
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RazorEdge ® Common Specifications
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Filter Types for Raman Spectroscopy
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MaxLine ® Laser-line Spectra and S
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Near Infrared Bandpass Filters Tran
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StopLine ® Single-notch Filter Com
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TECHNICAL NOTE Filter Spectra at No
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Working with Optical Density Optica
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LEARN MORE WITH OUR VIDEO COLLECTIO
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The Physics of Pixel Shift Pixel sh
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Get the Right Solution, Right Now W
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