Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Cubes Laser<br />
Sets<br />
NLO<br />
Filters<br />
TECHNICAL NOTE<br />
BrightLine ® LED-Optimized Fluorescence Filter Sets<br />
Over the past decade, LED-based light engines have largely replaced gas-discharge lamps (or arc lamps) for fluorescence<br />
imaging because LEDs enable faster channel switching, deliver discrete output wavelengths, consume less energy, and offer<br />
significantly longer lifetimes. An important factor to consider in this change in excitation technology is that the spectral<br />
signature and energy distribution of LED light engines differ from those of arc lamps. This is illustrated in Figure 1.<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
LED Broadband (Lumencor SOLA light engine)<br />
Lamp Xenon Arc (Xenon Corp)<br />
10<br />
0<br />
350 450 550 650 750<br />
Wavelength (nm)<br />
Figure 1: “Comparison of the spectra of a Xenon arc lamp with a Broadband<br />
LED light engine”<br />
BrightLine LED-based Light Engine Filter Set Solutions<br />
Because traditional filter sets are tuned to match the<br />
output spectra of arc lamps, the mismatch between<br />
the illumination spectra of the new LED lamps with<br />
the transmission spectra of the traditional filter sets<br />
causes a reduction in the brightness of the illumination<br />
reaching the fluorophores. As an example, consider<br />
the case of the DA/FI/TR/Cy5-4X-B (traditional) quadband<br />
filter set. The excitation filters in this set are<br />
optimized for the output spectrum of Xenon (Xe)<br />
or Mercury (Hg) arc lamps. When the light source is<br />
changed to an LED light engine of similar brightness,<br />
the overall transmitted energy through the four<br />
excitation channels is reduced, and within the Cy5<br />
band, the excitation intensity is extremely low.<br />
To address the loss of transmitted energy caused by spectrum mismatch, <strong>Semrock</strong> developed a new generation of filter sets<br />
that match the excitation filters to the unique spectral peaks of the most popular LED-based light engines on the market<br />
today. Take, for example, the Cy5 channel of the previously mentioned quad-band set. Figure 2 shows the improvement of<br />
transmitted energy when, for an LED light engine, the selected excitation filter is changed from the FF01-650/13 (traditional<br />
Cy5 exciter) to the corresponding FF01-635/18 (LED Cy5<br />
exciter). It can be seen that the peak for the FF01-635/18<br />
100<br />
has been centered over the LED peak.<br />
90<br />
Designing new excitation filters for the LED-optimized sets<br />
has resulted in improvement of the transmitted signal for<br />
the DAPI and TRITC bands by 35% each in comparison to<br />
the traditional sets. Most importantly, the signal within the<br />
Cy5 band is restored, with an improvement of 750%.<br />
Transmission (%)<br />
80<br />
70<br />
60<br />
50<br />
40<br />
LED Broadband<br />
(Lumencor SOLA light engine)<br />
FF01-650/13<br />
FF01-635/18<br />
30<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
The <strong>Semrock</strong> BrightLine Benefit<br />
The new generation of LED-optimized BrightLine filter sets<br />
offers several benefits over traditional multiband filter sets:<br />
Excellent Signal Quality: Excitation filters are spectrally<br />
positioned to deliver the brightest signal and the best<br />
signal-to-noise ratio in the industry.<br />
20<br />
10<br />
0<br />
350 450 550 650<br />
Wavelength (nm)<br />
Figure 2: “SearchLight simulation comparing the transmission through<br />
a FF01-650/13 (Cy5 exciter of traditional multiband filter set) and a FF01-<br />
635/18 (Cy5 exciter of the multiband set optimized for LED Light Engines)<br />
when illuminated by a typical LED light engine.”<br />
Reduced Complexity and Cost: Common excitation filters can be used for both single-band and corresponding multiband<br />
configurations. This interchangeability provides a seamless transition between single-band and multiband imaging when<br />
configuring the excitation filters into the LED-based light engine and can also provide significant cost savings.<br />
Wide Selection: The BrightLine LED filter set family includes over twenty filter sets, supporting single-band, full multiband,<br />
Pinkel, and Sedat configurations. The filter sets include penta-band (5 channel), quad-band (4-channel), and triple-band (3<br />
channel) configurations for the most popular fluorophores.<br />
750<br />
Tunable<br />
Filters<br />
Affordable Eight-Color Imaging: The penta-band LED-DA/FI/TR/Cy5/Cy7-5X-A and triple-band LED-CFP/YFP/mCherry-3X-A<br />
filter sets have complementary channels that provide an easy off-the-shelf solution to image eight fluorophores within a<br />
sample. The separation among all eight channels of the penta- and triple-band filters makes eight-color imaging easy and<br />
affordable (Figure 3).<br />
(continued)<br />
More<br />
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