Semrock Master Catalog 2018

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Fluorophores Single-band Sets BrightLine ® Basic TM Best-value Single-band Sets How can You do Great Research on a Tight Budget? BrightLine Basic Fluorescence Filter Sets! Hard-coated Performance at Soft-coated Prices. These value-priced single-band filter sets combine the proven durability of BrightLine research sets with optical performance that exceeds premium soft-coated fluorescence filters, yet are offered at soft-coated prices. In fact, BrightLine Basic filter sets are brighter than soft-coated filter sets of comparable contrast, but don’t burn out, further lowering the total cost of ownership. Ideal for routine applications that require cost-effective, high volume capabilities and no burn-out such as: clinical microscopy (mycological and fungal staining, immunofluorescent testing), routine analysis, and education. Multiband Sets Cubes Laser Sets Measured data taken on an Olympus BX microscope using a 40X objective and a QImaging Retiga camera. Sample is Invitrogen / Molecular Probes FluoCells #2 sample (BODIPY FL fluorophore). Brightness & Signal-to-Noise Ratio (Relative) 1.0 0.8 0.6 0.4 0.2 0.0 BrightLine Basic FITC-A-Basic Competitor Premium Soft-coated FITC Set Relative Brightness Relative Signal-to-Noise Ratio Competitor Standard Soft-coated FITC Set BrightLine® (Highest Performance) set compared to BrightLine Basic (Best Value) set NLO Filters Individual Filters Dichroic Beamsplitters Tunable Filters <strong>Semrock</strong>’s highest-performance BrightLine filter sets offer the best fluorescence filters available, while the value-priced BrightLine Basic filter sets provide a high level of performance and same proven durability at an outstanding price. BrightLine Filter Set $785 $575 DAPI-1160B DAPI-5060C BrightLine Basic Filter Set BFP-A-Basic BrightLine Filter Set Compared to BrightLine Basic Filter Set* >10% higher brightness; >10% higher contrast (using BFP) Several times brighter; comparable contrast (using BFP) CFP-2432C CFP-A-Basic Tens of percent higher brightness; comparable contrast GFP-3035D GFP-A-Basic Tens of percent higher contrast; brightness slightly lower FITC-3540C FITC-A-Basic >10% higher brightness; >10% higher contrast YFP-2427B YFP-A-Basic Tens of percent higher brightness; comparable contrast TRITC-B TRITC-A-Basic Tens of percent higher brightness and contrast; Basic set intentionally designed for traditional deep-red TRITC emission TXRED-4040C TXRED-A-Basic >10% higher brightness; >10% higher contrast Cy5-4040C Cy5-A-Basic > 5% higher brightness; comparable contrast (using Alexa Fluor ® 647) • Only sets which have corresponding BrightLine and BrightLine Basic sets are listed. • Brightness is based on relative throughput using the primary fluorophore and assuming typical metal-halide lamp and CCD camera spectral responses. • Contrast is the signal-to-noise ratio (SNR), assuming the background noise is dominated by broadband autofluorescence (as is typically the case in moderate to higher fluorophore concentration samples). * Actual results may vary depending on instrumentation and the exact sample preparation, which can substantially impact the spectra and relative intensities of the fluorophore and background. More 24
BrightLine ® Basic TM Best-value Single-band Sets Filter Set / Primary Fluorophores CFW-LP01-Clinical (Longpass) Calcofluor White, DAPI Mycological and fungal staining tests CFW-BP01-Clinical Calcofluor White, DAPI Mycological and fungal staining tests BFP-A-Basic BFP, DAPI, Hoechst, AMCA, Alexa Fluor ® 350 CFP-A-Basic CFP, AmCyan, SYTOX Blue, BOBO-1, PO-PRO-1 WGFP-A-Basic wtGFP GFP-A-Basic GFP, (EGFP), DiO, Cy2 , YOYO-1, YO-PRO-1 FITC-A-Basic FITC, rsGFP, Bodipy, 5-FAM, Fluo-4, Alexa Fluor ® 488 FITC-LP01-Clinical (Longpass) FITC, Acridine Orange Immunofluorescent clinical tests Excitation (CWL/BW) Emission (CWL/BW) Dichroic (Edge) Filter Set Part Numbers Base Price 387/11 416/LP 409 nm CFW-LP01-Clinical-000 $575 387/11 442/46 409 nm CFW-BP01-Clinical-000 $575 390/18 460/60 416 nm BFP-A-Basic-000 $575 434/17 479/40 452 nm CFP-A-Basic-000 $575 445/45 510/42 482 nm WGFP-A-Basic-000 $575 469/35 525/39 497 nm GFP-A-Basic-000 $575 475/35 530/43 499 nm FITC-A-Basic-000 $575 475/28 515/LP 500 nm FITC-LP01-Clinical-000 $575 Fluorophores Single-band Sets Multiband Sets Cubes YFP-A-Basic YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123 497/16 535/22 516 nm YFP-A-Basic-000 $575 TRITC-A-Basic TRITC, dTomato, Alexa Fluor ® 546 Cy3.5-A-Basic Cy3.5 , mStrawberry TXRED-A-Basic Texas Red ® , mCherry, 5-ROX, Alexa Fluor ® 568, mRFP1 Cy5-A-Basic Cy5 , Alexa Fluor ® 647, SpectrumFRed 542/20 620/52 570 nm TRITC-A-Basic-000 $575 565/24 620/52 585 nm CY3.5-A-Basic-000 $575 559/34 630/69 585 nm TXRED-A-Basic-000 $575 630/38 694/44 655 nm Cy5-A-Basic-000 $575 Filter Specifications on page 34 Laser Sets NLO Filters See spectra graphs and ASCII data for these filter sets at www.semrock.com/Basic-SingleBand.aspx CUBES PAGE 35 Individual Filters Transmission (%) 100 90 80 70 60 50 40 30 20 10 BFP-A Basic Spectra Exciter Emitter Dichroic 0 300 350 400 450 500 550 Wavelength (nm) Transmission (%) FITC-LP01-Clinical Spectra 100 90 Dichroic 80 70 60 Exciter Emitter 50 40 30 20 10 0 400 450 500 550 600 650 Wavelength (nm) Dichroic Beamsplitters Tunable Filters 25 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21 and 22: BrightLine ® Single-band Sets for
Page 23 and 24: Qdot ® Single-band Filter Sets Fil
Page 25: BrightLine ® FRET Single-band Sets
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
Page 73 and 74: BrightLine ® Super-resolution / TI
Page 75 and 76: BrightLine ® Laser Multiedge Dichr
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LaserMUX TM Beam Combining Filters
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TECHNICAL NOTE Measurement of Optic
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VersaChrome ® Tunable Filters TECH
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VersaChrome ® Tunable Bandpass Fil
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VersaChrome Edge TM Tunable Filters
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Laser Wavelength Reference Table La
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Polarization Filters Common Specifi
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General Purpose Mirrors Semrock gen
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EdgeBasic TM Long / Short Wave Pass
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Edge Steepness and Transition Width
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RazorEdge ® Common Specifications
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Filter Types for Raman Spectroscopy
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MaxLine ® Laser-line Spectra and S
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Near Infrared Bandpass Filters Tran
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StopLine ® Single-notch Filter Com
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TECHNICAL NOTE Filter Spectra at No
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Working with Optical Density Optica
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LEARN MORE WITH OUR VIDEO COLLECTIO
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The Physics of Pixel Shift Pixel sh
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Get the Right Solution, Right Now W
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Semrock Master Catalog 2018

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