Semrock Master Catalog 2018

Fluorophores
Single-band
Sets
BrightLine ® Basic TM Best-value Single-band Sets
How can You do Great Research on a Tight Budget?
BrightLine Basic Fluorescence Filter Sets!
Hard-coated Performance at Soft-coated Prices.
These value-priced single-band filter sets combine the proven durability of BrightLine research sets
with optical performance that exceeds premium soft-coated fluorescence filters, yet are offered
at soft-coated prices. In fact, BrightLine Basic filter sets are brighter than soft-coated filter sets of
comparable contrast, but don’t burn out, further lowering the total cost of ownership. Ideal for
routine applications that require cost-effective, high volume capabilities and no burn-out such as:
clinical microscopy (mycological and fungal staining, immunofluorescent testing), routine analysis,
and education.
Multiband
Sets
Cubes Laser
Sets
Measured data taken on an Olympus BX
microscope using a 40X objective and a
QImaging Retiga camera. Sample is
Invitrogen / Molecular Probes FluoCells #2
sample (BODIPY FL fluorophore).
Brightness & Signal-to-Noise Ratio (Relative)
1.0
0.8
0.6
0.4
0.2
0.0
BrightLine Basic
FITC-A-Basic
Competitor
Premium
Soft-coated
FITC Set
Relative Brightness
Relative Signal-to-Noise Ratio
Competitor
Standard
Soft-coated
FITC Set
BrightLine® (Highest Performance) set compared to BrightLine Basic (Best Value) set
NLO
Filters
Individual
Filters
Dichroic
Beamsplitters
Tunable
Filters
Semrock’s highest-performance BrightLine filter sets offer the best fluorescence filters available, while the value-priced
BrightLine Basic filter sets provide a high level of performance and same proven durability at an outstanding price.
BrightLine
Filter Set
$785 $575
DAPI-1160B
DAPI-5060C
BrightLine Basic
Filter Set
BFP-A-Basic
BrightLine Filter Set Compared to BrightLine Basic Filter Set*
>10% higher brightness; >10% higher contrast (using BFP)
Several times brighter; comparable contrast (using BFP)
CFP-2432C CFP-A-Basic Tens of percent higher brightness; comparable contrast
GFP-3035D GFP-A-Basic Tens of percent higher contrast; brightness slightly lower
FITC-3540C FITC-A-Basic >10% higher brightness; >10% higher contrast
YFP-2427B YFP-A-Basic Tens of percent higher brightness; comparable contrast
TRITC-B
TRITC-A-Basic
Tens of percent higher brightness and contrast;
Basic set intentionally designed for traditional deep-red TRITC emission
TXRED-4040C TXRED-A-Basic >10% higher brightness; >10% higher contrast
Cy5-4040C Cy5-A-Basic > 5% higher brightness; comparable contrast (using Alexa Fluor ® 647)
• Only sets which have corresponding BrightLine and BrightLine Basic sets are listed.
• Brightness is based on relative throughput using the primary fluorophore and assuming typical metal-halide lamp and CCD camera spectral responses.
• Contrast is the signal-to-noise ratio (SNR), assuming the background noise is dominated by broadband autofluorescence (as is typically the case in
moderate to higher fluorophore concentration samples).
* Actual results may vary depending on instrumentation and the exact sample preparation, which can substantially impact the spectra and relative intensities
of the fluorophore and background.
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