Semrock Master Catalog 2018

Qdot ® Single-band Filter Sets
Filter Set /
Primary Fluorophores
Cell image courtesy of
Thermo Fisher Scientific.
Excitation
(CWL/BW)
Emission
(CWL/BW)
These single-band filter sets are specially optimized for
brilliant, dense multi-color detection with Molecular
Probes ® (Thermo Fisher Scientific) quantum dot nanocrystals.
The highly transmitting, deep-blue exciter achieves maximum
quantum dot excitation efficiency while virtually eliminating
any DAPI or Hoechst excitation. And with the no burn-out
reliability shared by all BrightLine ® filters, the permanent
performance of these sets will outlast even your
quantum dots!
Dichroic
(Edge)
Filter Set Part Numbers /
–ZERO Set Part Numbers
QDLP-C (Longpass) 435/40 500/LP 510 nm QDLP-C-000 $785
Qdot ®
525, 565, 585, 605, 625, 655, 705, & 800 Nanocrystals
Versatile and high brightness longpass filter set for viewing multiple Qdots
QD525-C
Qdot ® 525 Nanocrystals
QD605-C
Qdot ® 605 Nanocrystals
QD625-C
Qdot ® 625 Nanocrystals
QD655-C
Qdot ® 655 Nanocrystals
See spectra graphs and ASCII data for these
filter sets at www.semrock.com/Qdot.aspx
435/40 525/15 510 nm QD525-C-000
QD525-C-000-ZERO
435/40 605/15 510 nm QD605-C-000
QD605-C-000-ZERO
435/40 625/15 510 nm QD625-C-000
QD625-C-000-ZERO
435/40 655/15 510 nm QD655-C-000
QD655-C-000-ZERO
Base Price /
–ZERO Price
$785
$884
$785
$884
$785
$884
$785
$884
Filter Specifications on page 34
“–ZERO” denotes zero pixel shift performance (see page 36)
Fluorophores
Single-band
Sets
Multiband
Sets
Cubes
Laser
Sets
TECHNICAL NOTE
Fluorescence Imaging with Quantum Dot Nanocrystals
Quantum dot nanocrystals are fluorophores that absorb photons of light and then re-emit
longer-wavelength photons nearly instantaneously. However, there are some important
differences between quantum dots (e.g., Qdot ®
nanocrystals made by Thermo Fisher
Scientific) and traditional fluorophores including organic dyes and naturally fluorescing
proteins. Quantum dots are nanometer-scale clusters of semiconductor atoms, typically
coated with an additional semiconductor shell and then a polymer coating to enable coupling
to proteins, oligonucleotides, small molecules, etc., which are then used for direct binding of
the quantum dots to targets of interest.
Nanocrystals are extremely bright and highly photostable, making them ideal for applications that require
high sensitivity with minimal label interference, as well as long-term photostability, such as live-cell imaging and dynamic
studies. Their excellent photostability also means they are fixable and archivable for permanent sample storage in pathology
applications. Because there is a direct relationship between the size of a nanocrystal and the wavelength of the emitted
fluorescence, a full range of nanocrystals can be made – each with a narrow, distinct emission spectrum and all excited by a
single blue or ultraviolet wavelength. Thus nanocrystals are ideal for dense multiplexing. Some important nanocrystal features
that may limit certain applications include their fairly large physical size and long lifetime.
Transmission (%)
100
90
80
70
60
50
40
30
20
10
0
350 400 450 500 550 600 650 700
Wavelength (nm)
Figure 1. Structure of a nanocrystal.
To take advantage of nanocrystal features, it is important to use properly
optimized filters. Semrock offers BrightLine ®
filter sets specially optimized for
the most popular quantum dot imaging applications. A universal set with a
long-wave-pass emitter enables simultaneous imaging of multiple quantum
dots by eye or with a color camera. Additionally, filter sets tailored to individual
quantum dots are also available (see filter sets above). Best of all, these filters
share the incredible “no burn-out” reliability of all BrightLine filters, an ideal
match for highly photostable quantum dot nanocrystals!
Figure 2. A universal exciter provides superior excitation efficiency while avoiding the excitation of DAPI
and undesirable autofluorescence. This filter is combined with a dichroic beamsplitter with extremely wide
reflection and transmission bands for maximum flexibility, and narrow, highly transmitting emission filters
matched to each of the most important Qdot wavelengths.
NLO
Filters
Individual
Filters
Dichroic
Beamsplitters
Tunable
Filters
21
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