Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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Qdot ® Single-band Filter Sets<br />
Filter Set /<br />
Primary Fluorophores<br />
Cell image courtesy of<br />
Thermo Fisher Scientific.<br />
Excitation<br />
(CWL/BW)<br />
Emission<br />
(CWL/BW)<br />
These single-band filter sets are specially optimized for<br />
brilliant, dense multi-color detection with Molecular<br />
Probes ® (Thermo Fisher Scientific) quantum dot nanocrystals.<br />
The highly transmitting, deep-blue exciter achieves maximum<br />
quantum dot excitation efficiency while virtually eliminating<br />
any DAPI or Hoechst excitation. And with the no burn-out<br />
reliability shared by all BrightLine ® filters, the permanent<br />
performance of these sets will outlast even your<br />
quantum dots!<br />
Dichroic<br />
(Edge)<br />
Filter Set Part Numbers /<br />
–ZERO Set Part Numbers<br />
QDLP-C (Longpass) 435/40 500/LP 510 nm QDLP-C-000 $785<br />
Qdot ®<br />
525, 565, 585, 605, 625, 655, 705, & 800 Nanocrystals<br />
Versatile and high brightness longpass filter set for viewing multiple Qdots<br />
QD525-C<br />
Qdot ® 525 Nanocrystals<br />
QD605-C<br />
Qdot ® 605 Nanocrystals<br />
QD625-C<br />
Qdot ® 625 Nanocrystals<br />
QD655-C<br />
Qdot ® 655 Nanocrystals<br />
See spectra graphs and ASCII data for these<br />
filter sets at www.semrock.com/Qdot.aspx<br />
435/40 525/15 510 nm QD525-C-000<br />
QD525-C-000-ZERO<br />
435/40 605/15 510 nm QD605-C-000<br />
QD605-C-000-ZERO<br />
435/40 625/15 510 nm QD625-C-000<br />
QD625-C-000-ZERO<br />
435/40 655/15 510 nm QD655-C-000<br />
QD655-C-000-ZERO<br />
Base Price /<br />
–ZERO Price<br />
$785<br />
$884<br />
$785<br />
$884<br />
$785<br />
$884<br />
$785<br />
$884<br />
Filter Specifications on page 34<br />
“–ZERO” denotes zero pixel shift performance (see page 36)<br />
Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Cubes<br />
Laser<br />
Sets<br />
TECHNICAL NOTE<br />
Fluorescence Imaging with Quantum Dot Nanocrystals<br />
Quantum dot nanocrystals are fluorophores that absorb photons of light and then re-emit<br />
longer-wavelength photons nearly instantaneously. However, there are some important<br />
differences between quantum dots (e.g., Qdot ®<br />
nanocrystals made by Thermo Fisher<br />
Scientific) and traditional fluorophores including organic dyes and naturally fluorescing<br />
proteins. Quantum dots are nanometer-scale clusters of semiconductor atoms, typically<br />
coated with an additional semiconductor shell and then a polymer coating to enable coupling<br />
to proteins, oligonucleotides, small molecules, etc., which are then used for direct binding of<br />
the quantum dots to targets of interest.<br />
Nanocrystals are extremely bright and highly photostable, making them ideal for applications that require<br />
high sensitivity with minimal label interference, as well as long-term photostability, such as live-cell imaging and dynamic<br />
studies. Their excellent photostability also means they are fixable and archivable for permanent sample storage in pathology<br />
applications. Because there is a direct relationship between the size of a nanocrystal and the wavelength of the emitted<br />
fluorescence, a full range of nanocrystals can be made – each with a narrow, distinct emission spectrum and all excited by a<br />
single blue or ultraviolet wavelength. Thus nanocrystals are ideal for dense multiplexing. Some important nanocrystal features<br />
that may limit certain applications include their fairly large physical size and long lifetime.<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
350 400 450 500 550 600 650 700<br />
Wavelength (nm)<br />
Figure 1. Structure of a nanocrystal.<br />
To take advantage of nanocrystal features, it is important to use properly<br />
optimized filters. <strong>Semrock</strong> offers BrightLine ®<br />
filter sets specially optimized for<br />
the most popular quantum dot imaging applications. A universal set with a<br />
long-wave-pass emitter enables simultaneous imaging of multiple quantum<br />
dots by eye or with a color camera. Additionally, filter sets tailored to individual<br />
quantum dots are also available (see filter sets above). Best of all, these filters<br />
share the incredible “no burn-out” reliability of all BrightLine filters, an ideal<br />
match for highly photostable quantum dot nanocrystals!<br />
Figure 2. A universal exciter provides superior excitation efficiency while avoiding the excitation of DAPI<br />
and undesirable autofluorescence. This filter is combined with a dichroic beamsplitter with extremely wide<br />
reflection and transmission bands for maximum flexibility, and narrow, highly transmitting emission filters<br />
matched to each of the most important Qdot wavelengths.<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
21<br />
More