Semrock Master Catalog 2018

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Fluorophores Single-band Sets Multiband Sets Cubes Laser Sets NLO Filters Individual Filters Dichroic Beamsplitters Tunable Filters BrightLine ® Single-band Sets for FISH & Dense Multiplexing Filter Set / Primary Fluorophores SpAqua-C SpectrumAqua , DEAC SpGr-B SpectrumGreen , FITC, Alexa Fluor ® 488 SpGold-B SpectrumGold , Alexa Fluor ® 532 SpOr-B SpectrumOrange , Cy3 , Rhodamine, Alexa Fluor ® 555 SpRed-B SpectrumRed , Texas Red, Alexa Fluor ® 668 & 594 Excitation (CWL/BW) Emission (CWL/BW) Dichroic (Edge) Filter Set Part Numbers / –ZERO Set Part Numbers 438/24 483/32 458 nm SpAqua-C-000 SpAqua-C-000-ZERO 494/20 527/20 506 nm SpGr-B-000 SpGr-B-000-ZERO 534/20 572/28 552 nm SpGold-B-000 SpGold-B-000-ZERO 543/22 586/20 562 nm SpOr-B-000 SpOr-B-000-ZERO 586/20 628/32 605 nm SpRed-B-000 SpRed-B-000-ZERO NOTE: For DAPI , Cy5 TM , Cy5.5 TM , or Cy7 TM sets, refer to pages 15-16. ® BrightLine Filter Set / Primary Fluorophores DA/SpGr/SpRed-C (Combination set) Blue: DAPI Multiband/Combination Sets for FISH Single-band Excitation (CWL/BW) Multiband Excitation (CWL/BW) Base Price / –ZERO Price $785 $884 $785 $884 $785 $884 $785 $884 $785 $884 Filter Specifications on page 34 “–ZERO” denotes zero pixel shift performance (see page 36) Multiband Emission (CWL/BW) Multiedge Dichroic (Edge) Ex1: 387/11 Ex5: 407/14 457/22 436 nm Green: FITC, Spectrum Green Ex2: 494/20 Ex4: 494/20 Ex5: 494/20 530/20 514 nm Filter Set Part Numbers Base Price DA/SpGr/SpRed-C-000 $2265 Red: Spectrum Red, Texas Red Ex3: 575/25 Ex4: 576/20 628/28 604 nm Ex5: 576/20 Filter Set features three individual exciters, a dual-band exciter, and a triple-band exciter, plus one triple-band emitter, and one multiedge dichroic. DA-SpAq/SpGr/SpOr-A (Combination set) Blue: DAPI Ex1: 340/26 465/30 444 nm Cyan: CFP, Spectrum Aqua Ex2: 427/10 Ex6: 422/30 465/30 444 nm Green: FITC, Spectrum Green Ex3: 504/12 Ex5: 503/18 Ex6: 503/18 Orange: Spectrum Orange Ex4: 575/15 Ex5: 572/18 Ex6: 572/18 537/20 520 nm 623/50 590 nm DA-SpAq/SpGr/ SpOr-A-000 Filter Set features four individual exciters, a dual-band exciter, and a triple-band exciter, plus one triple-band emitter, and one multiedge dichroic See spectra graphs and ASCII data for these filter sets at www.semrock.com/FISH-SingleBand.aspx and www.semrock.com/FISH-MultiBand.aspx $2395 More 20
Qdot ® Single-band Filter Sets Filter Set / Primary Fluorophores Cell image courtesy of Thermo Fisher Scientific. Excitation (CWL/BW) Emission (CWL/BW) These single-band filter sets are specially optimized for brilliant, dense multi-color detection with Molecular Probes ® (Thermo Fisher Scientific) quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. And with the no burn-out reliability shared by all BrightLine ® filters, the permanent performance of these sets will outlast even your quantum dots! Dichroic (Edge) Filter Set Part Numbers / –ZERO Set Part Numbers QDLP-C (Longpass) 435/40 500/LP 510 nm QDLP-C-000 $785 Qdot ® 525, 565, 585, 605, 625, 655, 705, & 800 Nanocrystals Versatile and high brightness longpass filter set for viewing multiple Qdots QD525-C Qdot ® 525 Nanocrystals QD605-C Qdot ® 605 Nanocrystals QD625-C Qdot ® 625 Nanocrystals QD655-C Qdot ® 655 Nanocrystals See spectra graphs and ASCII data for these filter sets at www.semrock.com/Qdot.aspx 435/40 525/15 510 nm QD525-C-000 QD525-C-000-ZERO 435/40 605/15 510 nm QD605-C-000 QD605-C-000-ZERO 435/40 625/15 510 nm QD625-C-000 QD625-C-000-ZERO 435/40 655/15 510 nm QD655-C-000 QD655-C-000-ZERO Base Price / –ZERO Price $785 $884 $785 $884 $785 $884 $785 $884 Filter Specifications on page 34 “–ZERO” denotes zero pixel shift performance (see page 36) Fluorophores Single-band Sets Multiband Sets Cubes Laser Sets TECHNICAL NOTE Fluorescence Imaging with Quantum Dot Nanocrystals Quantum dot nanocrystals are fluorophores that absorb photons of light and then re-emit longer-wavelength photons nearly instantaneously. However, there are some important differences between quantum dots (e.g., Qdot ® nanocrystals made by Thermo Fisher Scientific) and traditional fluorophores including organic dyes and naturally fluorescing proteins. Quantum dots are nanometer-scale clusters of semiconductor atoms, typically coated with an additional semiconductor shell and then a polymer coating to enable coupling to proteins, oligonucleotides, small molecules, etc., which are then used for direct binding of the quantum dots to targets of interest. Nanocrystals are extremely bright and highly photostable, making them ideal for applications that require high sensitivity with minimal label interference, as well as long-term photostability, such as live-cell imaging and dynamic studies. Their excellent photostability also means they are fixable and archivable for permanent sample storage in pathology applications. Because there is a direct relationship between the size of a nanocrystal and the wavelength of the emitted fluorescence, a full range of nanocrystals can be made – each with a narrow, distinct emission spectrum and all excited by a single blue or ultraviolet wavelength. Thus nanocrystals are ideal for dense multiplexing. Some important nanocrystal features that may limit certain applications include their fairly large physical size and long lifetime. Transmission (%) 100 90 80 70 60 50 40 30 20 10 0 350 400 450 500 550 600 650 700 Wavelength (nm) Figure 1. Structure of a nanocrystal. To take advantage of nanocrystal features, it is important to use properly optimized filters. <strong>Semrock</strong> offers BrightLine ® filter sets specially optimized for the most popular quantum dot imaging applications. A universal set with a long-wave-pass emitter enables simultaneous imaging of multiple quantum dots by eye or with a color camera. Additionally, filter sets tailored to individual quantum dots are also available (see filter sets above). Best of all, these filters share the incredible “no burn-out” reliability of all BrightLine filters, an ideal match for highly photostable quantum dot nanocrystals! Figure 2. A universal exciter provides superior excitation efficiency while avoiding the excitation of DAPI and undesirable autofluorescence. This filter is combined with a dichroic beamsplitter with extremely wide reflection and transmission bands for maximum flexibility, and narrow, highly transmitting emission filters matched to each of the most important Qdot wavelengths. NLO Filters Individual Filters Dichroic Beamsplitters Tunable Filters 21 More
Page 1 and 2: Semrock 2018 Master Catalog Everyth
Page 3 and 4: Welcome to the 2018 Master Catalog
Page 5 and 6: T T T T Laser & Optical System Filt
Page 7 and 8: SearchLight Optimization Calculator
Page 9 and 10: The Semrock Advantage Proven Reliab
Page 11 and 12: BrightLine ® Single-band Sets When
Page 13 and 14: BrightLine ® Single-band Sets for
Page 15 and 16: BrightLine ® Multiband Sets for Po
Page 17 and 18: BrightLine ® Single-band Sets Filt
Page 19 and 20: TECHNICAL NOTE Ultraviolet (UV) Flu
Page 21: BrightLine ® Single-band Sets for
Page 25 and 26: BrightLine ® FRET Single-band Sets
Page 27 and 28: BrightLine ® Basic TM Best-value S
Page 29 and 30: (continued from previous page) Fluo
Page 31 and 32: BrightLine ® Multiband LED Filter
Page 33 and 34: BrightLine ® Multiband Fluorescenc
Page 35 and 36: BrightLine ® Multiband Fluorescenc
Page 37 and 38: Fluorescence Filter Cubes See onlin
Page 39 and 40: BrightLine ® Laser Filter and Set
Page 41 and 42: BrightLine ® Laser Fluorescence Mu
Page 43 and 44: BrightLine ® Laser Fluorescence Fi
Page 45 and 46: TECHNICAL NOTE Sputtered Thin-film
Page 47 and 48: BrightLine ® Multiphoton LaserMUX
Page 49 and 50: Multiphoton Filters Common Specific
Page 51 and 52: BrightLine ® Single-band Bandpass
Page 59 and 60: BrightLine ® Long / Short pass Sin
Page 61 and 62: BrightLine ® Multiband Bandpass Fi
Page 63 and 64: BrightLine ® Multiband Bandpass Fi
Page 65 and 66: BrightLine ® Single-edge Dichroic
Page 67 and 68: BrightLine ® Multiedge Dichroic Be
Page 69 and 70: BrightLine ® Dichroic Beamsplitter
Page 71 and 72: Single-band Sets Fluorophores (cont
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BrightLine ® Super-resolution / TI
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BrightLine ® Laser Multiedge Dichr
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LaserMUX TM Beam Combining Filters
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TECHNICAL NOTE Measurement of Optic
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VersaChrome ® Tunable Filters TECH
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VersaChrome ® Tunable Bandpass Fil
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VersaChrome Edge TM Tunable Filters
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Laser Wavelength Reference Table La
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Polarization Filters Common Specifi
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General Purpose Mirrors Semrock gen
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EdgeBasic TM Long / Short Wave Pass
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Edge Steepness and Transition Width
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RazorEdge ® Common Specifications
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Filter Types for Raman Spectroscopy
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MaxLine ® Laser-line Spectra and S
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Near Infrared Bandpass Filters Tran
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StopLine ® Single-notch Filter Com
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TECHNICAL NOTE Filter Spectra at No
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Working with Optical Density Optica
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LEARN MORE WITH OUR VIDEO COLLECTIO
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The Physics of Pixel Shift Pixel sh
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Get the Right Solution, Right Now W
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