Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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BrightLine ® Single-band Sets for FISH & Dense Multiplexing<br />
PathVysion ®<br />
assay control sample<br />
with CEP 17 and HER-2/neu probes<br />
(100X oil-immersion objective).<br />
Help Ease the Upstream Battle Against Cancer<br />
with BrightLine FISH Fluorescence Filter Sets<br />
Fluorescence In Situ Hybridization, or FISH, is an exciting fluorescence imaging technique that<br />
enables clinical-scale genetic screening based on molecular diagnostics. <strong>Semrock</strong> pioneered<br />
hard-coated BrightLine filters that are significantly brighter than and have superior contrast to older,<br />
soft-coated fluorescence filters, thus offering faster and more accurate measurements. Independent<br />
evaluations have shown that FISH images can be obtained in as little as one half the exposure time<br />
using BrightLine filters. And yet the inherent manufacturability of <strong>Semrock</strong>’s patented ion-beamsputtered<br />
filters actually allows them to be priced lower than soft-coated FISH filter sets.<br />
Switching to BrightLine filters is the simplest and least expensive way to dramatically increase the<br />
quality of your FISH images.<br />
Full Spectrum of Solutions<br />
Examples of popular assays using BrightLine FISH filter sets<br />
Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Single-band Filter Sets Assay Purpose<br />
■■■ DAPI, SpGr, SpOr PathVysion ® Detects amplification of the HER-2 gene for screening and<br />
prognosis of breast cancer<br />
■■■■ DAPI, SpAqua, SpGr, SpOr AneuVysion ® Used as an aid in prenatal diagnosis of chromosomal<br />
abnormalities<br />
■■■■■ DAPI, SpAqua, SpGr, SpGold, SpRed UroVysion Aid for initial diagnosis of bladder carcinoma and<br />
subsequent monitoring for tumor recurrence in<br />
previously diagnosed patients<br />
■■■■■■ DAPI, SpAqua, SpGr, SpGold,<br />
SpRed, Cy5<br />
M-FISH<br />
Permits the simultaneous visualization of all human (or mouse)<br />
chromosomes in different colors for karyotype analysis<br />
Cubes<br />
Laser<br />
Sets<br />
PRODUCT NOTE<br />
Can Better Fluorescence Filters Really Make a Difference?<br />
NLO<br />
Filters<br />
BrightLine “no-burn-out” filters have been tested widely in both research and clinical fields over many years of use.<br />
Extensive independent testing has also been performed with BrightLine FISH filter sets. A few examples of results are<br />
shown here. Whether you are finding and analyzing metaphase spreads or scoring cells by spot counting, significantly<br />
improve the speed and accuracy of your FISH analysis with BrightLine filter sets.<br />
Competitor filter set<br />
BrightLine filter set<br />
Side-by-side independent comparison using equal exposure times<br />
of images achieved with competitor filter sets (left) and BrightLine<br />
filter sets (right), of a human tumor hybridized with CEP 3 probe in<br />
Spectrum Red (part of Vysis UroVysion TM<br />
assay, 400X magnification).<br />
Photo courtesy of Tina Bocker Edmonston, M.D., Thomas<br />
Jefferson University.<br />
Integration Time (relative)<br />
1.0<br />
0.8<br />
0.6<br />
0.4<br />
0.2<br />
0.0<br />
Spectrum<br />
Aqua<br />
Competitor FISH Filter Set<br />
BrightLine FISH Filter Set<br />
Spectrum<br />
Green<br />
Spectrum<br />
Red<br />
BrightLine filters allow shorter integration times for faster imaging – especially<br />
for automated tasks like metaphase finding. This independent industry test<br />
compares integration times required by BrightLine FISH filter sets to those of<br />
competitor filter sets. The automated system, based on a Zeiss Axio Imager<br />
microscope, found metaphase spreads with identical image intensities.<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
19<br />
More