Semrock Master Catalog 2018

BrightLine ® Single-band Sets for FISH & Dense Multiplexing
PathVysion ®
assay control sample
with CEP 17 and HER-2/neu probes
(100X oil-immersion objective).
Help Ease the Upstream Battle Against Cancer
with BrightLine FISH Fluorescence Filter Sets
Fluorescence In Situ Hybridization, or FISH, is an exciting fluorescence imaging technique that
enables clinical-scale genetic screening based on molecular diagnostics. Semrock pioneered
hard-coated BrightLine filters that are significantly brighter than and have superior contrast to older,
soft-coated fluorescence filters, thus offering faster and more accurate measurements. Independent
evaluations have shown that FISH images can be obtained in as little as one half the exposure time
using BrightLine filters. And yet the inherent manufacturability of Semrock’s patented ion-beamsputtered
filters actually allows them to be priced lower than soft-coated FISH filter sets.
Switching to BrightLine filters is the simplest and least expensive way to dramatically increase the
quality of your FISH images.
Full Spectrum of Solutions
Examples of popular assays using BrightLine FISH filter sets
Fluorophores
Single-band
Sets
Multiband
Sets
Single-band Filter Sets Assay Purpose
■■■ DAPI, SpGr, SpOr PathVysion ® Detects amplification of the HER-2 gene for screening and
prognosis of breast cancer
■■■■ DAPI, SpAqua, SpGr, SpOr AneuVysion ® Used as an aid in prenatal diagnosis of chromosomal
abnormalities
■■■■■ DAPI, SpAqua, SpGr, SpGold, SpRed UroVysion Aid for initial diagnosis of bladder carcinoma and
subsequent monitoring for tumor recurrence in
previously diagnosed patients
■■■■■■ DAPI, SpAqua, SpGr, SpGold,
SpRed, Cy5
M-FISH
Permits the simultaneous visualization of all human (or mouse)
chromosomes in different colors for karyotype analysis
Cubes
Laser
Sets
PRODUCT NOTE
Can Better Fluorescence Filters Really Make a Difference?
NLO
Filters
BrightLine “no-burn-out” filters have been tested widely in both research and clinical fields over many years of use.
Extensive independent testing has also been performed with BrightLine FISH filter sets. A few examples of results are
shown here. Whether you are finding and analyzing metaphase spreads or scoring cells by spot counting, significantly
improve the speed and accuracy of your FISH analysis with BrightLine filter sets.
Competitor filter set
BrightLine filter set
Side-by-side independent comparison using equal exposure times
of images achieved with competitor filter sets (left) and BrightLine
filter sets (right), of a human tumor hybridized with CEP 3 probe in
Spectrum Red (part of Vysis UroVysion TM
assay, 400X magnification).
Photo courtesy of Tina Bocker Edmonston, M.D., Thomas
Jefferson University.
Integration Time (relative)
1.0
0.8
0.6
0.4
0.2
0.0
Spectrum
Aqua
Competitor FISH Filter Set
BrightLine FISH Filter Set
Spectrum
Green
Spectrum
Red
BrightLine filters allow shorter integration times for faster imaging – especially
for automated tasks like metaphase finding. This independent industry test
compares integration times required by BrightLine FISH filter sets to those of
competitor filter sets. The automated system, based on a Zeiss Axio Imager
microscope, found metaphase spreads with identical image intensities.
Individual
Filters
Dichroic
Beamsplitters
Tunable
Filters
19
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