Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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TECHNICAL NOTE<br />
Ultraviolet (UV) Fluorescence Applications<br />
Many biological molecules of interest naturally fluoresce when excited by shorter<br />
wavelength UV light. This “intrinsic fluorescence” can be a powerful tool as labeling<br />
with extrinsic fluorophores is not required. One important application is the direct<br />
fluorescence imaging of aromatic amino acids including tryptophan, tyrosine, and<br />
phenylalanine, which are building blocks for proteins. The aromatic rings in these<br />
molecules give rise to strong fluorescence excitation peaks in the 260 to 280 nm<br />
range. Another application is DNA quantitation. Purines and pyrimidines – bases for<br />
nucleic acids like DNA and RNA – have strong absorption bands in the 260 to<br />
280 nm range.<br />
<strong>Semrock</strong>’s UV BrightLine fluorescence filters offer a powerful tool for direct<br />
fluorescence imaging. These unique UV filters are reliable “no burn-out” and offer<br />
performance nearly comparable to visible and near-IR filters. Figure 1 shows the<br />
spectrum of a high-reliability 280 nm BrightLine excitation filter with the highest<br />
commercially available transmission (> 65%), remarkably steep edges, and wideband<br />
blocking across the entire UV and visible spectrum. This spectrum is directly compared<br />
to a traditional and inferior metal-dielectric filter. In one example system, this filter<br />
difference was shown to provide over 100x improvement in signal-to-noise ratio.<br />
Figure 2 shows the spectra from a UV filter set designed for imaging tryptophan,<br />
overlaid on the absorption and emission spectra for that amino acid. Note the<br />
nearly ideal overlap and high transmission of all three filters in this set.<br />
100<br />
UV Near-UV Visible (VIS)<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
BrightLine FF01-280/20 UV filter<br />
Traditional metal-dielectric filter<br />
30<br />
20<br />
10<br />
0<br />
200 300 400 500 600 700<br />
Wavelength (nm)<br />
Figure 1. BrightLine FF01-280/20-25 filter<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
Exciter<br />
Dichroic<br />
70<br />
Emitter<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
250 275 300 325 350 375 400 425 450<br />
Wavelength (nm)<br />
Figure 2. TRP-A single-band fluorescence filter set is<br />
ideal for imaging tryptophan (see filter set above).<br />
Transmission (%)<br />
Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Cubes<br />
ACTUAL MEASURED DATA<br />
What you see is what you get. The published spectra for our filters are actual measured data from actual finished parts,<br />
not theory and not a design estimate only to be made under ideal conditions. Our strict manufacturing control and<br />
advanced metrology capabilities ensure that the filter you receive meets the spectral specifications you expect.<br />
Laser<br />
Sets<br />
Highest Contrast FITC-2024B Set<br />
Highest Brightness FITC-5050A Set<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
400<br />
Dichroic<br />
Exciter<br />
Emitter<br />
425 450 475 500 525 550 575 600<br />
Wavelength (nm)<br />
Transmission (%)<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
400<br />
Dichroic<br />
Exciter<br />
Emitter<br />
425 450 475 500 525 550 575<br />
Wavelength (nm)<br />
600<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
INDIVIDUAL & FILTER SET PACKAGING<br />
<strong>Semrock</strong> utilizes individual PET-G packaging for standard-size<br />
catalog filters. This packaging allows <strong>Semrock</strong> to produce cleaner<br />
parts, reduce potential defects caused by shipping & handling, and<br />
utilize 100% recyclable PET materials.<br />
Custom size filters in low volume will be packaged in envelopes lined<br />
with cleanroom grade, non-abrasive, resin-free, non-woven fabric.<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
Learn more about our packaging at www.semrock.com/packaging.aspx<br />
17<br />
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