Semrock Master Catalog 2018

Fluorophores
Single-band
Sets
Multiband
Sets
BrightLine ® Single-band Sets
Filter Set /
Primary Fluorophores
TRITC-B
TRITC, Rhodamine, DiI,
5-TAMRA, Alexa Fluor ® 532 & 546
Cy3-4040C
Cy3 , DsRed, PE, 5-TAMRA, Calcium Orange,
Alexa Fluor ® 555
TXRED-4040C
Texas Red ® , Cy3.5 , 5-ROX, Mitotracker Red,
Alexa Fluor ® 568 & 594
mCherry-40LP-A (Longpass)
mCherry, mRFP1
mCherry-C
mCherry, mRFP
Cy5-4040C
Cy5 , APC, DiD, Alexa Fluor ® 647 & 660
Excitation
(CWL/BW)
Emission
(CWL/BW)
Dichroic
(Edge)
Filter Set Part Numbers /
–ZERO Set Part Numbers
543/22 593/40 562 nm TRITC-B-000
TRITC-B-000-ZERO
531/40 593/40 562 nm Cy3-4040C-000
Cy3-4040C-000-ZERO
562/40 624/40 593 nm TXRED-4040C-000
TXRED-4040C-000-ZERO
562/40 593/LP 593 nm mCherry-40LP-A-000
mCherry-40LP-000-ZERO
562/40 641/75 593 nm mCherry-C-000
mCherry-C-000-ZERO
628/40 692/40 660 nm Cy5-4040-C-000
Cy5-4040-C-000-ZERO
Base Price /
–ZERO Price
$785
$884
$785
$884
$785
$884
$785
$884
$785
$884
$785
$884
Cubes Laser
Sets
Cy5.5-C
Cy5.5 , Alexa Fluor ® 680 & 700
Cy7-B
Cy7 , Alexa Fluor ® 750
IRDYE800-33LP-A (Longpass)
IRDye800 CW, DyLight 800
ICG-B
Indocyanin Green
CUBES PAGE 35
655/40 716/40 685 nm Cy5.5-C-000
Cy5.5-C-000-ZERO
708/75 809/81 757 nm Cy7-B-000
Cy7-B-000-ZERO
747/33 776/LP 776 nm IRDYE800-33LP-A-000
IRDYE800-33LP-A-000-
ZERO
769/41 832/37 801 nm ICG-B-000
ICG-B-000-ZERO
$785
$884
$785
$884
$785
$884
$785
$884
Filter Specifications on page 34
“–ZERO” denotes zero pixel shift performance (see page 36)
NLO
Filters
Individual
Filters
Dichroic
Beamsplitters
Tunable
Filters
TECHNICAL NOTE
Using Fura-2 to Track Ca²+ Using VersaChrome ® Filters
The fluorophore Fura-2 has an absorption spectrum that varies markedly depending on the concentration of calcium (Ca²+)
that is present near the fluorophore molecule. By measuring the ratio of intensities on digital images captured using two
different excitation wavelengths, the variation of calcium concentration as a function of location on the sample can be tracked.
The conventional approach to ratiometric imaging with Fura-2 is based on exchanging two excitation filters in a filter wheel
(see above for the FURA2-C set). Now with Semrock’s VersaChrome TBP01-378/16 tunable excitation filter, ratiometric
imaging with Fura-2 can be more carefully optimized to your specific experimental conditions. This filter makes it possible
to monitor the Fura-2 signal corresponding to virtually any excitation wavelength between 340 and 378 nm – continuous
tuning like a monochromator, yet with the high transmission, steep edges, and high out-of-band blocking available only with
optical filters. Wavelength tuning of a VersaChrome filter can be 2 to 3 times faster than exchanging filters with even the
highest-speed filter wheel.
The added flexibility of a tunable excitation
source can even enable new types of
experiments. For example, the same excitation
filter used for high-contrast ratiometric imaging
can be tuned to excite Fura-2 at its isosbestic
point, thus enabling monitoring of the Fura-2
signal independent of calcium concentration.
Transmission (%)
Saturated Ca 2+ Free Ca 2+
100
90
80
70
60
50
40
30
20
Tunable
Exciter
60°
45°
30°
0°
Emitter
Dichroic
10
0
300 350 400 450 500 550 600
Wavelength (nm)
Signal [Arb. Units]
x 10 -9
7
6
5
4
3
2
1
Isosbestic
Point
Free Ca 2+
2
Saturated Ca 2+
0
0
340 345 350 355 360 365 370 375 380
Wavelength (nm)
Ratio
7
6
5
4
3
1
Ratio: “Free” / “Saturated”
More
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