Semrock Master Catalog 2018
Semrock Master Catalog 2018
Semrock Master Catalog 2018
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Fluorophores<br />
Single-band<br />
Sets<br />
Multiband<br />
Sets<br />
Cubes Laser<br />
Sets<br />
NLO<br />
Filters<br />
Individual<br />
Filters<br />
Dichroic<br />
Beamsplitters<br />
Tunable<br />
Filters<br />
BrightLine ® Single-band Sets for Popular Fluorophores<br />
Primary Fluorophores<br />
5-FAM<br />
(5-carboxyfluorescein)<br />
5-ROX<br />
(carboxy-X-rhodamine)<br />
5-TAMRA<br />
(5-carboxytetramethylrhodamine,<br />
high pH > 8)<br />
Peak<br />
EX<br />
Peak<br />
EM<br />
492 518<br />
578 604<br />
Alexa Fluor ® 350 343 441<br />
Alexa Fluor ® 405 402 422<br />
Alexa Fluor ® 488 499 520<br />
Recommended<br />
BrightLine Sets<br />
FITC-3540C<br />
LED-FITC-A<br />
TXRED-4040C<br />
TXRED-A-Basic<br />
Page<br />
16<br />
28<br />
17<br />
25<br />
542 568 TRITC-B 17<br />
DAPI-1160B<br />
DAPI-5060C<br />
BFP-A-Basic<br />
DAPI-1160B<br />
DAPI-5060C<br />
LED-DAPI-A<br />
FITC-3540C<br />
FITC-A-Basic<br />
15<br />
15<br />
25<br />
15<br />
15<br />
28<br />
Alexa Fluor ® 532 534 553 TRITC-B 17<br />
Alexa Fluor ® 546 556 572<br />
TRITC-B<br />
TRITC-A-Basic<br />
Alexa Fluor ® 555 553 565 Cy3-4040C 17<br />
Alexa Fluor ® 568 579 603<br />
Alexa Fluor ® 594 590 618<br />
Alexa Fluor ® 647 653 668<br />
Alexa Fluor ® 660 663 691<br />
TXRED-4040C<br />
TXRED-A-Basic<br />
TXRED-4040C<br />
TXRED-A-Basic<br />
Cy5-4040C<br />
Cy5-A-Basic<br />
Cy5-4040C<br />
Cy5-A-Basic<br />
Alexa Fluor ® 680 679 702 Cy5.5-C 17<br />
Alexa Fluor ® 750 751 776 Cy7-B 17<br />
AMCA / AMCA-X 350 448<br />
DAPI-1160B<br />
DAPI-5060C<br />
BFP-A-Basic<br />
AmCyan 458 489 CFP-2432C 16<br />
BFP (EBFP) 380 440<br />
BODIPY 505 512<br />
BD Horizon Brillant TM<br />
Ultraviolet 395<br />
BD Horizon Brillant TM<br />
Violet 421<br />
BD Horizon Brillant TM<br />
Violet 480<br />
Calcofluor White 349 439<br />
Cascade Blue TM 401 419<br />
CFP (cyan GFP) 433 475<br />
DAPI-1160B<br />
DAPI-5060C<br />
BFP-A-Basic<br />
LED-DAPI-A<br />
LF405-C<br />
FITC-3540C<br />
FITC-A-Basic<br />
16<br />
25<br />
17<br />
25<br />
17<br />
25<br />
17<br />
25<br />
17<br />
25<br />
17<br />
25<br />
15<br />
15<br />
25<br />
15<br />
15<br />
25<br />
28<br />
38<br />
16<br />
25<br />
348 395 BUV395-3018A 15<br />
407 421 BV421-3824A 15<br />
440 479 BV480-2432A 15<br />
DAPI-1160B<br />
DAPI-5060C<br />
CFW-LP01<br />
CFW-BP01<br />
DAPI-1160B<br />
DAPI-5060C<br />
CFP-2432C<br />
CFP-A-Basic<br />
LED-CFP-A<br />
15<br />
15<br />
25<br />
25<br />
15<br />
15<br />
16<br />
25<br />
28<br />
Primary Fluorophores<br />
Peak<br />
EX<br />
Peak<br />
EM<br />
Cerulean 434 473<br />
CoralHue Kusabira<br />
Orange (mKO)<br />
Cy2 TM 492 507<br />
Cy3 TM 554 566<br />
Cy3.5 TM 578 591<br />
Cy5 TM 649 666<br />
Recommended<br />
BrightLine Sets<br />
CFP-2432C<br />
CFP-A-Basic<br />
LED-CFP-A<br />
LF442-C<br />
Page<br />
16<br />
25<br />
28<br />
38<br />
549 559 Cy3-4040C 17<br />
GFP-3035D<br />
GFP-A-Basic<br />
LED-FITC-A<br />
LF488-D<br />
Cy3-4040C<br />
LF561-B<br />
Cy3.5-A-Basic<br />
TXRED-4040C<br />
Cy5-4040C<br />
Cy5-A-Basic<br />
LED-Cy5-A<br />
LF635-C<br />
16<br />
25<br />
28<br />
38<br />
17<br />
38<br />
25<br />
17<br />
17<br />
25<br />
28<br />
38<br />
Cy5.5 TM 672 690 Cy5.5-C 17<br />
Cy7 TM 753 775<br />
DAPI 359 461<br />
Cy7-B<br />
LED-C y7-A<br />
DAPI-1160B<br />
DAPI-5060C<br />
BFP-A-Basic<br />
LED-DAPI-A<br />
LF405-C<br />
17<br />
28<br />
15<br />
15<br />
25<br />
28<br />
38<br />
DEAC 432 472 SpAqua-C 20<br />
DsRed Monomer 559 583<br />
DsRed2 563 582<br />
DsRed-Express 556 584<br />
dTomato 556 582<br />
Cy3-4040C<br />
LF561-C<br />
Cy3-4040C<br />
LF561-C<br />
Cy3-4040C<br />
LF561-C<br />
TRITC-B<br />
Cy3-4040C<br />
TRITC-A-Basic<br />
LED-TRITC-A<br />
LF561-C<br />
17<br />
38<br />
17<br />
38<br />
17<br />
38<br />
17<br />
17<br />
25<br />
28<br />
38<br />
DyLight 800 777 794 IRDYE800-33LP-A 17<br />
Emerald 491 511<br />
FITC (Fluorescein) 495 519<br />
Fluo-3 506 527<br />
Fura-2<br />
393,<br />
338<br />
512,<br />
505<br />
FITC-3540C<br />
GFP-3035D<br />
FITC-3540C<br />
FITC-A-Basic<br />
LED-FITC-A<br />
LF488-C<br />
YFP-2427B<br />
YFP-A-Basic<br />
LF514-C<br />
16<br />
16<br />
16<br />
25<br />
28<br />
38<br />
17<br />
25<br />
38<br />
FURA2-C 16<br />
Fura Red TM (high pH) 572 657 TXRED-4040C 17<br />
GFP (EGFP) 489 511<br />
GFP-3035D<br />
GFP-A-Basic<br />
LED-FITC-A<br />
LF488-D<br />
HcRed 588 618 TXRED-4040C 17<br />
Hoechst 33258<br />
Hoechst 33342<br />
Hoechst 34580<br />
352<br />
350<br />
392<br />
455<br />
462<br />
440<br />
DAPI-1160B<br />
DAPI-5060C<br />
BFP-A-Basic<br />
16<br />
25<br />
28<br />
38<br />
15<br />
15<br />
25<br />
More<br />
10