Semrock Master Catalog 2018

Polarizers
Semrock White Paper Abstract Library
Full downloadable versions are available on our website, www.semrock.com/white-papers.aspx
Mirrors NIR Filters
Edge
Filters
Dichroic
Beamsplitters
Laser-line
Filters
Laser Diode
Filters
Notch
Filters
Lamp Clean-up
Filters
Maximizing the Performance
of Advanced Microscopes
by Controlling Wavefront
Error Using Optical Filters
Wavefront distortion can degrade
image quality by reducing contrast or
compromising resolution. In several
microscopy applications, reducing
wavefront distortion is critical to
achieving the microscopy method.
Specifying and selecting optical filters
that minimize wavefront aberration
is important to maximize or enable
optical system performance. This article
elucidates how to select optical filters
for high performance microscopy,
and provides guidance on choosing
Semrock catalog filters for wavefront
distortion performance required
for applications.
Super-resolution Microscopy
The latest incarnation of the modern
fluorescence microscope has led to
a paradigm shift. This wave is about
breaking the diffraction limit first
proposed in 1873 by Ernst Abbe and
the implications of this development
are profound. This new technology,
called super-resolution microscopy,
allows for the visualization of cellular
samples with a resolution similar to
that of an electron microscope, yet it
retains the advantages of an optical
fluorescence microscope.
Optical Filters for Laserbased
Fluorescence
Microscopes
Lasers are increasingly and
advantageously replacing broadband
light sources for many fluorescence
imaging applications. However,
fluorescence applications based on
lasers impose new constraints on
imaging systems and their components.
For example, optical filters used
confocal and Total Internal Reflection
Fluorescence (TIRF) microscopes
have specific requirements that are
unique compared to those filters
used in broadband light source
based instruments.
Filter Sets for Next
Generation Microscopy
LED-based light engines are gaining
in popularity for fluorescence imaging.
However, the full potential of LED light
engines remains to be realized in most
imaging configurations because they
are still being used with conventional
filter sets designed for mercury or
xenon arc lamps. Semrock’s LED-based
light engine filter sets are aligned to
the unique spectral peaks of the most
popular LED-based light engines on the
market today.
Fluorescent Proteins:
Theory, Applications
and Best Practices
The latest incarnation of the modern
fluorescence microscope has led to
a paradigm shift. This wave is about
breaking the diffraction limit first
proposed in 1873 by Ernst Abbe and
the implications of this development
are profound. This new technology,
called super-resolution microscopy,
allows for the visualization of cellular
samples with a resolution similar to
that of an electron microscope, yet it
retains the advantages of an optical
fluorescence microscope.
Spectral Modeling in
Fluorescence Microscopy
SearchLight is a free, online spectrum
plotting and analysis tool that allows
fluorescence microscope users and
optical instrument designers to model
and evaluate the spectral performance
of fluorophores, filter sets, light
sources, and detectors as components
of an overall system. This white paper
provides the theoretical basis for the
SearchLight calculations, illustrating
the individual aspects with academic
precision, but also with very useful
insights into practical problems related
to noise in biological fluorescence
microscopy systems.
Spectral Imaging
with VersaChrome
Spectral imaging with linear unmixing
is necessary in multicolor fluorescence
imaging when fluorophore spectra
are highly overlapping. Tunable
fluorescence filters now enable spectral
imaging with all the advantages of thinfilm
filters, including high transmission
with steep spectral edges and high outof-band
blocking.
Creating Your Own
Bandpass Filter
Semrock’s VersaChrome Edge filters
unlock virtually unlimited spectral
flexibility for fluorescence microscopy
and hyperspectral imaging as well as
spectroscopy applications. By utilizing
a combination of VersaChrome Edge
tunable long-wave-pass and shortwave-pass
filters, a bandpass filter
as narrow as sub 5nm FWHM or as
wide as 12% of the center wavelength
throughout the visible and near-infrared
wavelength ranges can be created.
Semrock VersaChrome
Tunable Bandpass Filters
Many optical systems can benefit from
tunable filters with the spectral and
two-dimensional imaging performance
characteristics of thinfilm filters and the
center wavelength tuning speed and
flexibility of a diffraction grating.
Flatness of Dichroic
Beamsplitters Affects
Focus & Image Quality
Dichroic beamsplitters are now used
as “image-splitting” elements for
many applications, such as live-cell
imaging and FRET, in which both the
transmitted and reflected signals are
imaged onto a camera. The optical
quality of such dichroics is critical
to achieving high-quality images,
especially for the reflected light. If the
beamsplitter is not sufficiently flat, then
significant optical aberrations may be
introduced and the imaging may be
severely compromised.
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