Rubella Virus IgG Immunoblot - NovaTec Immundiagnostica GmbH

Rubella Virus IgG Immunoblot
Rubella virus is the causative agent of ‘German Measles’, a classical children’s
disease. Symptoms are often mild, and up to 50 % of infections may be subclinical
or unapparent. However, maternal rubella infection in early pregnancy can cause
severe embryopathies.
Rubella virus, an enveloped single-stranded RNA virus, belongs to the Togaviridae
family and is the sole member of the genus Rubivirus. It occurs worldwide, and
humans are its only known reservoir.
Rubella virus is transmitted by the respiratory route. It replicates in cells of the
respiratory tract, extends to the local lymph nodes, and then spreads throughout the body. Approximately 8 days
after primary infection the virus can be detected in the blood and in respiratory secretions. Finally a maculopapular
rash appears on the face and spreads across the body accompanied by mild fever. Complications like encephalitis
and persisting arthropathies are rare. Virus is excreted in nasopharyngeal secretions and in the urine.
When a woman acquires rubella virus during pregnancy it can lead to severe consequences to the fetus,
especially in the first trimester of pregnancy. Maternal viremia may result in infection of the placenta and the fetus.
Possible outcomes are miscarriage, fetal death, or Congenital Rubella Syndrome (CRS).
The classic triad of CRS consists of sensorineural deafness (58 % of patients), eye abnormalities - especially
cataract and microphthalmia (43 % of patients) and congenital heart disease - especially patent ductus arteriosus
(50 % of patients).
Species Disease Symptoms Mechanism of infection
Rubella
virus
German Measles
Congenital Rubella
Syndrome (Gregg's
syndrome)
NovaTec Immundiagnostica GmbH
Technologie & Waldpark
Waldstrasse 23 A6
D-63128 Dietzenbach, Germany
Exanthema (fever, feeling of
sickness)
Cardiovascular lesions, eye
damage, hearing defect, CNS
involvement etc.
Infection may be diagnosed by:
� Detection of virus by RT-PCR (prenatal)
� Hemagglutination inhibition (HAI), Haemolysis-in-gel test (HIG)
� Serology: EIA, ELISA, immunoblot
Rubella Virus IgG Immunoblot:
Phone: +49 (0) 6074 4876 – 0
Fax: +49 (0) 6074 4876 – 29
E-mail: info@novatec-id.com
Internet: www.novatec-id.com
Transmission by droplet infection
Fetal infection:
Transplacental infection during
maternal viremia
The NovaTec Rubella Virus IgG Immunoblot is intended for the qualitative determination of IgG-class antibodies
against rubellavirus in human serum. Antibodies specific for the E2 structural antigen can serologically rule out a
rubella virus infection within the last three months.
Antigens:
Rubella K2S antigen
Version 08.2009

Specific performance characteristics:
Sensitivity % Specificity %
IgG > 98 > 98
Order information:
Blot Number of determinations Product number
Rubella Virus IgG Immunoblot 10 RUBG2400
Rubella Virus IgG Immunoblot Benefits:
� K2S antigen for high sensitivity
� Identification of antigen-bands via kit-specific template
� Ready-to-use, color-coded reagents
� Assay procedure 2.5 hours
Rubella Serology Timeline
Infectivity
Viremia
0 2 4
6 8
► Also Available:
days
IgM
IgG
3 months
NovaTec Immundiagnostica GmbH
Technologie & Waldpark
Waldstrasse 23 A6
D-63128 Dietzenbach, Germany
E2-IgG
Rubella Virus IgG - ELISA (Product number: RUBG0400)
Rubella Virus IgM µ-capture (Product number: RUBM0400)
1 2 3 years
Phone: +49 (0) 6074 4876 – 0
Fax: +49 (0) 6074 4876 – 29
E-mail: info@novatec-id.com
Internet: www.novatec-id.com
Version 08.2009