Rubella Virus IgG Immunoblot - NovaTec Immundiagnostica GmbH
Rubella Virus IgG Immunoblot - NovaTec Immundiagnostica GmbH
Rubella Virus IgG Immunoblot - NovaTec Immundiagnostica GmbH
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<strong>Rubella</strong> <strong>Virus</strong> <strong>IgG</strong> <strong>Immunoblot</strong><br />
<strong>Rubella</strong> virus is the causative agent of ‘German Measles’, a classical children’s<br />
disease. Symptoms are often mild, and up to 50 % of infections may be subclinical<br />
or unapparent. However, maternal rubella infection in early pregnancy can cause<br />
severe embryopathies.<br />
<strong>Rubella</strong> virus, an enveloped single-stranded RNA virus, belongs to the Togaviridae<br />
family and is the sole member of the genus Rubivirus. It occurs worldwide, and<br />
humans are its only known reservoir.<br />
<strong>Rubella</strong> virus is transmitted by the respiratory route. It replicates in cells of the<br />
respiratory tract, extends to the local lymph nodes, and then spreads throughout the body. Approximately 8 days<br />
after primary infection the virus can be detected in the blood and in respiratory secretions. Finally a maculopapular<br />
rash appears on the face and spreads across the body accompanied by mild fever. Complications like encephalitis<br />
and persisting arthropathies are rare. <strong>Virus</strong> is excreted in nasopharyngeal secretions and in the urine.<br />
When a woman acquires rubella virus during pregnancy it can lead to severe consequences to the fetus,<br />
especially in the first trimester of pregnancy. Maternal viremia may result in infection of the placenta and the fetus.<br />
Possible outcomes are miscarriage, fetal death, or Congenital <strong>Rubella</strong> Syndrome (CRS).<br />
The classic triad of CRS consists of sensorineural deafness (58 % of patients), eye abnormalities - especially<br />
cataract and microphthalmia (43 % of patients) and congenital heart disease - especially patent ductus arteriosus<br />
(50 % of patients).<br />
Species Disease Symptoms Mechanism of infection<br />
<strong>Rubella</strong><br />
virus<br />
German Measles<br />
Congenital <strong>Rubella</strong><br />
Syndrome (Gregg's<br />
syndrome)<br />
<strong>NovaTec</strong> <strong>Immundiagnostica</strong> <strong>GmbH</strong><br />
Technologie & Waldpark<br />
Waldstrasse 23 A6<br />
D-63128 Dietzenbach, Germany<br />
Exanthema (fever, feeling of<br />
sickness)<br />
Cardiovascular lesions, eye<br />
damage, hearing defect, CNS<br />
involvement etc.<br />
Infection may be diagnosed by:<br />
� Detection of virus by RT-PCR (prenatal)<br />
� Hemagglutination inhibition (HAI), Haemolysis-in-gel test (HIG)<br />
� Serology: EIA, ELISA, immunoblot<br />
<strong>Rubella</strong> <strong>Virus</strong> <strong>IgG</strong> <strong>Immunoblot</strong>:<br />
Phone: +49 (0) 6074 4876 – 0<br />
Fax: +49 (0) 6074 4876 – 29<br />
E-mail: info@novatec-id.com<br />
Internet: www.novatec-id.com<br />
Transmission by droplet infection<br />
Fetal infection:<br />
Transplacental infection during<br />
maternal viremia<br />
The <strong>NovaTec</strong> <strong>Rubella</strong> <strong>Virus</strong> <strong>IgG</strong> <strong>Immunoblot</strong> is intended for the qualitative determination of <strong>IgG</strong>-class antibodies<br />
against rubellavirus in human serum. Antibodies specific for the E2 structural antigen can serologically rule out a<br />
rubella virus infection within the last three months.<br />
Antigens:<br />
<strong>Rubella</strong> K2S antigen<br />
Version 08.2009
Specific performance characteristics:<br />
Sensitivity % Specificity %<br />
<strong>IgG</strong> > 98 > 98<br />
Order information:<br />
Blot Number of determinations Product number<br />
<strong>Rubella</strong> <strong>Virus</strong> <strong>IgG</strong> <strong>Immunoblot</strong> 10 RUBG2400<br />
<strong>Rubella</strong> <strong>Virus</strong> <strong>IgG</strong> <strong>Immunoblot</strong> Benefits:<br />
� K2S antigen for high sensitivity<br />
� Identification of antigen-bands via kit-specific template<br />
� Ready-to-use, color-coded reagents<br />
� Assay procedure 2.5 hours<br />
<strong>Rubella</strong> Serology Timeline<br />
Infectivity<br />
Viremia<br />
0 2 4<br />
6 8<br />
► Also Available:<br />
days<br />
IgM<br />
<strong>IgG</strong><br />
3 months<br />
<strong>NovaTec</strong> <strong>Immundiagnostica</strong> <strong>GmbH</strong><br />
Technologie & Waldpark<br />
Waldstrasse 23 A6<br />
D-63128 Dietzenbach, Germany<br />
E2-<strong>IgG</strong><br />
<strong>Rubella</strong> <strong>Virus</strong> <strong>IgG</strong> - ELISA (Product number: RUBG0400)<br />
<strong>Rubella</strong> <strong>Virus</strong> IgM µ-capture (Product number: RUBM0400)<br />
1 2 3 years<br />
Phone: +49 (0) 6074 4876 – 0<br />
Fax: +49 (0) 6074 4876 – 29<br />
E-mail: info@novatec-id.com<br />
Internet: www.novatec-id.com<br />
Version 08.2009