SzSA YearBook 2016/17

SZENT-GYÖRGYI JUNIOR MENTORS
NIKOLETTA NAGY
Department of Medical Genetics,
University of Szeged,
Address: Somogyi u. 4., H-6720 Szeged, Hungary
E: nagy.nikoletta@med.u-szeged.hu
nikoletta.nagy@gmail.com
T: +36 62/545-134
RESEARCH AREA
My main research interest is the identification of genetic
susceptibility factors predisposing to the development of
rare (monogenic) and common (multifactorial) skin diseases.
Currently I am working on a rare skin disease, Brooke-Spiegler
syndrome, characterized by the development
of significantly stigmatizing tumors of the skin appendages.
These studies would give the basis of the development
of future genetic and pharmacogenomics screening tests
with diagnostic or therapeutic purposes. Another field of
interest is the development and Research area of causative
treatment strategies such as cell-, gene-therapy methods.
TECHNIQUES AVAILABLE IN THE LAB
Basic DNA techniques (genomic and cell free DNA isolation,
PCR preparation, gel electrophoresis, sequencing, digital
PCR measurements), polymorphism studies in multifactorial
diseases, mutation screening in rare diseases, gene and
protein expression analysis (Q-RT-PCR, Western blot), isolation
and culturing of human cells, using skin organotypic
model, transfection into human cell lines, RNA silencing,
basic cell biology studies (migration, proliferation assays).
SELECTED PUBLICATIONS
Koumbaris, G., Kypri, E., Tsangaras, K., Achilleos, A., Mina,
P., Neofytou, M., Velissariou, V., Christopoulou, G., Kallikas,
I., González-Liñán, A., Benusiene, E., Latos-Bielenska, A.,
Marek, P., Santana, A., Nagy, N., Széll, M., Laudanski, P.,
Papageorgiou, E.A., Ioannides, M., Patsalis, P.C. (2016) Cell-
Free DNA Analysis of Targeted Genomic Regions in Maternal
Plasma for Non-Invasive Prenatal Testing of Trisomy 21,
Trisomy 18, Trisomy 13, and Fetal Sex. Clin Chem 62: 848-55.
Rebane, A., Runnel, T., Aab, A., Maslovskaja, J., Ruckert,
B., Zimmermann, M., Plaas, M., Kaner, J., Treis, A., Pihlap,
M., Haljasorg, U., Hermann, H., Nagy, N., Kemeny, L.,
Erm, T., Kingo, K., Li, M., Boldin, M.P., Akdis, C.A. (2014)
MicroRNA-146a alleviates chronic skin inflammation in
atopic dermatitis through suppression of innate immune
responses in keratinocytes. J Allergy Clin Immun 134: 836-
847.
Rebane, A., Zimmermann, M., Aab, A., Baurecht, H., Koreck,
A., Karelson, M., Abram, K., Metsalu, T., Pihlap, M., Meyer, N.,
Folster-Holst, R., Nagy, N., Kemeny, L,, Kingo, K., Vilo, J., Illig,
T., Akdis, M., Franke, A., Novak, N., Weidinger, S., Akdis, C.A,
(2012) Mechanisms of IFN-gamma-induced apoptosis of
human skin keratinocytes in patients with atopic dermatitis,
J Allergy Clin Immun 129: 1297-1306.
Tanaka, A. Weinel, S., Nagy, N., O’Driscoll, M., Lai-Cheong,
J.E., Kulp-Shorten, C.L., Knable, A., Carpenter, G., Fisher, S.A.,
Hiragun, M., Yanase, Y., Hide, M., Callen, J., McGrath, J.A.
(2012) Germline mutation in ATR in autosomal- dominant
oropharyngeal cancer syndrome. Am J Hum Genet 90: 511-
517.
Nagy, N., Almaani, N., Tanaka, A., Lai-Cheong, J.E.,
Techanukul, T., Mellerio, J.E., McGrath, J.A. (2011) HB-EGF
induces COL7A1 expression in keratinocytes and fibroblasts:
possible mechanism underlying allogeneic fibroblast
therapy in recessive dystrophic epidermolysis bullosa. J
Invest Dermatol 131: 1771-74.
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