SzSA YearBook 2016/17

SZENT-GYÖRGYI JUNIOR MENTORS
ANIKÓ GÖBLÖS
Department of Medical Genetics,
Department of Dermatology and Allergology,
University of Szeged
Address: Somogyi u. 4., H-6720 Szeged, Hungary
E: goblos.aniko@med.u-szeged.hu
T: +36 62/545-134
RESEARCH AREA
Genotyping has key importance for identifying the genetic
susceptibility factors and for understanding the pathogenesis
of human diseases. Our workgroup is focusing on
Parkinson’s disease (PD) which is the second most common
neurodegenerative disorder and affects 1% of the population
worldwide. The neural tissues express great amount of
long non-coding RNAs, where they play important role in
brain development, neuron function and maintenance, and
are also related to the development of neurodegenerative
diseases. We aimed to investigate the susceptibility roles of
lncRNAs gene polymorphisms in the development of PD.
In another project we investigate the genetics and molecular
susceptibility factors of multifactorial human skin diseases,
with a primary focus on psoriasis. By performing functional
analyses of these factors, we try to understand how
their mode of action leads to the pathogenesis of human
disease.
TECHNIQUES AVAILABLE IN THE LAB
SELECTED PUBLICATIONS
Göblös, A., Danis, J., Vas, K., Bata-Csörgő, Z., Kemény, L.,
Dobozy, A., Széll, M. (2016) Keratinocytes express functional
CARD18, a negative regulator of inflammasome activation,
and its altered expression in psoriasis may contribute to
disease pathogenesis. Mol immunol 73: 10-18.
Szegedi, K., Göblös, A., Bacsa, S., Antal, M., Németh, I.B.,
Bata-Csörgő, Z., Kemény, L., Dobozy, A., Széll, M. (2013)
Expression and Functional Studies on the Noncoding RNA,
PRINS. Int J Mol Sci 14: 205-225.
Szabó, E.Z., Manczinger, M., Göblös, A., Kemény, L., Lakatos,
L. (2012) Switching on RNA silencing suppressor activity by
restoring Argonaute binding to a viral protein. J Virol 86:
8324-7.
Gellért, L., Fuzik, J., Göblös, A., Sárközi, K., Marosi, M., Kis,
Z., Farkas, T., Szatmári, I., Fülöp, F., Vécsei, L., Toldi, J. (2011)
Neuroprotection with a new kynurenic acid analog in the
four-vessel occlusion model of ischemia. Eur J Pharmacol
667: 182-7.
After DNA isolation (from peripheral blood samples, cells
or tissue samples) the polymorphism analysis is carried out
using polymerase chain reaction (PCR) method.
The identified susceptibility factors are further analyzed
both on cellular and histological level using various primary
cells, cell lines and tissue samples.
For functional analyses, in vitro DNA techniques and gene-specific
silencing methods are used. Gene and protein
expression is assessed using PCR, western blot analysis,
immunohistochemistry and immunocytochemistry, flow
cytometry, and ELISA.
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